The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice
We evaluated the cyto/genotoxicity in peripheral blood of CD-1 mice exposed to different varieties of red wine Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo. Groups of five CD-1 mice were treated with: a) vehicle only; b) red wine or ethanol ad libitum (diluted 75% and 10%, r...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2017 |
| Country: | México |
| Institution: | UGTO |
| Repository: | Acta Universitaria |
| Language: | Spanish |
| OAI Identifier: | oai:www.actauniversitaria.ugto.mx:article/1221 |
| Online Access: | https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1221 |
| Access Level: | Open access |
| Keyword: | Vino tinto genotoxicidad micronúcleos citotoxicidad alcohol. Red wine genotoxicity micronucleus cytotoxicity |
| _version_ | 1878408964697227264 |
|---|---|
| author | García-Rodríguez, Maria del Carmen |
| author2 | Guerrero-Palomo, Gabriela Altamirano-Lozano, Mario |
| author2_role | author author |
| author_browse | Altamirano-Lozano, Mario García-Rodríguez, Maria del Carmen Guerrero-Palomo, Gabriela |
| author_facet | García-Rodríguez, Maria del Carmen Guerrero-Palomo, Gabriela Altamirano-Lozano, Mario |
| author_role | author |
| collection | Acta Universitaria |
| description | We evaluated the cyto/genotoxicity in peripheral blood of CD-1 mice exposed to different varieties of red wine Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo. Groups of five CD-1 mice were treated with: a) vehicle only; b) red wine or ethanol ad libitum (diluted 75% and 10%, respectively); c) undiluted red wine or ethanol diluted to 13.5% (two daily doses-0.25 ml via intragastric [i.g.]). The cyto/genotoxicity was evaluated by micronucleus (MN) assay, and by the relationship between polychromatic/monochromatic erythrocytes at 0 h, 24 h, 48 h and 72 h. Significant but slight increases of MN were observed only in the treatments i.g. of Cabernet Sauvignon, Merlot, and ethanol. However, the administration ad libitum of red wines tended to decrease the MN. Our findings suggest that the MN could be related with the bioavailability, pharmacodynamics, and pharmacokinetics of ethanol and the antioxidants of red wine. No cytotoxic effects were observed. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | MX_895fea71663cd850a3d31896db0bd013 |
| identifier_str_mv | 10.15174/au.2017.1221 |
| institution | UGTO |
| instname_str | UNIVERSIDAD DE GUANAJUATO |
| language | spa |
| network_acronym_str | MX |
| network_name_str | México |
| oai_identifier_str | oai:www.actauniversitaria.ugto.mx:article/1221 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | Universidad de Guanajuato |
| publisherStr | Universidad de Guanajuato |
| reponame_str | Acta Universitaria |
| spelling | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice Efecto de la administración intragástrica y ad libitum del vino tinto (Cabernet sauvignon, Merlot, Carménère y Cabernet sauvignon/Nebbiolo) y del alcohol en la cito/genotoxicidad evaluada en sangre periférica de ratones CD-1 García-Rodríguez, Maria del Carmen Guerrero-Palomo, Gabriela Altamirano-Lozano, Mario Vino tinto genotoxicidad micronúcleos citotoxicidad alcohol. Red wine genotoxicity micronucleus cytotoxicity We evaluated the cyto/genotoxicity in peripheral blood of CD-1 mice exposed to different varieties of red wine Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo. Groups of five CD-1 mice were treated with: a) vehicle only; b) red wine or ethanol ad libitum (diluted 75% and 10%, respectively); c) undiluted red wine or ethanol diluted to 13.5% (two daily doses-0.25 ml via intragastric [i.g.]). The cyto/genotoxicity was evaluated by micronucleus (MN) assay, and by the relationship between polychromatic/monochromatic erythrocytes at 0 h, 24 h, 48 h and 72 h. Significant but slight increases of MN were observed only in the treatments i.g. of Cabernet Sauvignon, Merlot, and ethanol. However, the administration ad libitum of red wines tended to decrease the MN. Our findings suggest that the MN could be related with the bioavailability, pharmacodynamics, and pharmacokinetics of ethanol and the antioxidants of red wine. No cytotoxic effects were observed. Se evaluó la cito/genotoxicidad en sangre periférica de ratones CD-1 expuestos a diferentes variedades de vino tinto (Cabernet Sauvignon, Merlot, Carménère y Cabernet Sauvignon/Nebbiolo). Grupos de cinco organismos fueron tratados: a) con el vehículo, b) vino tinto o etanol (diluidos al 75 y 10%, respectivamente) ad libitum, y c) vino tinto sin diluir o etanol diluido al 13.5% (dos dosis diarias-0.25 ml, vía i.g.). La genotoxicidad se evaluó con los micronúcleos (MN) y la citotoxicidad con la relación entre eritrocitos policromáticos/normocromáticos (EPC/ENC) a las 0, 24, 48 y 72 h. Solo se observaron incrementos significativos en los MN con los tratamientos i.g de Cabernet Sauvignon, Merlot y etanol, siendo mayor para el etanol. La administración ad libitum de los vinos tintos redujo los MN. Nuestros hallazgos sugieren que los efectos genotóxicos del vino tinto podrían estar relacionados con la biodisponibilidad, farmacodinámica y farmacocinética del etanol. No se observaron efectos citotóxicos. Universidad de Guanajuato https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1221 10.15174/au.2017.1221 |
| spellingShingle | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice García-Rodríguez, Maria del Carmen Vino tinto genotoxicidad micronúcleos citotoxicidad alcohol. Red wine genotoxicity micronucleus cytotoxicity |
| status_str | publishedVersion |
| title | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice |
| title_full | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice |
| title_fullStr | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice |
| title_full_unstemmed | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice |
| title_short | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice |
| title_sort | The effect of intragastric and ad libitum administration of red wines (Cabernet Sauvignon, Merlot, Carménère and Cabernet Sauvignon/Nebbiolo) and alcohol in the cyto/genotoxicity evaluated in the peripheral blood of CD-1 mice |
| topic | Vino tinto genotoxicidad micronúcleos citotoxicidad alcohol. Red wine genotoxicity micronucleus cytotoxicity |
| topic_facet | Vino tinto genotoxicidad micronúcleos citotoxicidad alcohol. Red wine genotoxicity micronucleus cytotoxicity |
| url | https://www.actauniversitaria.ugto.mx/index.php/acta/article/view/1221 |