Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines
The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | Brasil |
| Institution: | UFLA |
| Repository: | Repositório Institucional da UFLA |
| Language: | English |
| OAI Identifier: | oai:repositorio.ufla.br:1/39914 |
| Online Access: | https://repositorio.ufla.br/handle/1/39914 |
| Access Level: | Open access |
| Keyword: | Abiotic stress Zea mays qRT-PCR Water deficit Milho Análise fisiológica Expressão gênica Estresse abiótico Déficit hídrico |
| _version_ | 1878759795265110016 |
|---|---|
| author | Marques, Thaís Lima |
| author2 | Von Pinho, Renzo Garcia Von Pinho, Édila Vilela de Resende Paniago, Bruno da Costa Freitas, Natália Chagas Santos, Heloisa Oliveira dos |
| author2_role | author author author author author |
| author_browse | Freitas, Natália Chagas Marques, Thaís Lima Paniago, Bruno da Costa Santos, Heloisa Oliveira dos Von Pinho, Renzo Garcia Von Pinho, Édila Vilela de Resende |
| author_facet | Marques, Thaís Lima Von Pinho, Renzo Garcia Von Pinho, Édila Vilela de Resende Paniago, Bruno da Costa Freitas, Natália Chagas Santos, Heloisa Oliveira dos |
| author_role | author |
| bitstream_url_str_mv | https://repositorio.ufla.br/bitstreams/14367275-2983-49e2-bfbc-7c3e6c5dab97/download https://repositorio.ufla.br/bitstreams/730a8bad-1399-4a69-ab56-927c6c1675ba/download https://repositorio.ufla.br/bitstreams/b1270672-eb0f-44c0-b2c7-c69a76fef620/download |
| collection | Repositório Institucional da UFLA |
| description | The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | BR_8e2ebc98e70d64b805c7b5802eac92ed |
| institution | UFLA |
| instname_str | Universidade Federal de Lavras (UFLA) |
| language | eng |
| network_acronym_str | BR |
| network_name_str | Brasil |
| oai_identifier_str | oai:repositorio.ufla.br:1/39914 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Universidade Estadual de Maringá |
| publisherStr | Universidade Estadual de Maringá |
| reponame_str | Repositório Institucional da UFLA |
| spelling | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines Marques, Thaís Lima Von Pinho, Renzo Garcia Von Pinho, Édila Vilela de Resende Paniago, Bruno da Costa Freitas, Natália Chagas Santos, Heloisa Oliveira dos Abiotic stress Zea mays qRT-PCR Water deficit Milho Análise fisiológica Expressão gênica Estresse abiótico Déficit hídrico The objective of this study was to evaluate gene expression related to water deficit tolerance in maize lines. For this, lines previously classified as tolerant (91-T and 32-T) and non-tolerant (24-NT and 57-NT) to water deficit were used. The seeds of the four lines were evaluated for emergence and emergence speed index, and the seedlings were evaluated for root and shoot length under two conditions of water availability (70 and 10% substrate water retention capacity). In transcript analysis, the expression of several genes associated with water deficit tolerance, ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A, was evaluated by qRT-PCR for the 91-T and 57-NT lines. It can be concluded that soil water deficiency did not reduce root development. However, the shoot length was significantly lower under dry conditions. Through transcript analysis, the genes ZmDBP3 and ZmAN13 were identified as potential markers for the early selection of maize lines tolerant to water deficit. Universidade Estadual de Maringá MARQUES, T. L. et al. Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines. Acta Scientiarum. Agronomy, Maringá, v. 42, 2020. Paginação irregular. https://repositorio.ufla.br/handle/1/39914 |
| spellingShingle | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines Marques, Thaís Lima Abiotic stress Zea mays qRT-PCR Water deficit Milho Análise fisiológica Expressão gênica Estresse abiótico Déficit hídrico |
| status_str | publishedVersion |
| title | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines |
| title_full | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines |
| title_fullStr | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines |
| title_full_unstemmed | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines |
| title_short | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines |
| title_sort | Physiological analysis and gene expression analysis of ZmDBP3, ZmALDH9, ZmAN13, and ZmDREB2A in maize lines |
| topic | Abiotic stress Zea mays qRT-PCR Water deficit Milho Análise fisiológica Expressão gênica Estresse abiótico Déficit hídrico |
| topic_facet | Abiotic stress Zea mays qRT-PCR Water deficit Milho Análise fisiológica Expressão gênica Estresse abiótico Déficit hídrico |
| url | https://repositorio.ufla.br/handle/1/39914 |