Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw
To investigate the growth and production of lignocellulosic enzymes from Lentinus tigrinus, solid state fermentation (SSF) during 110 days was performed by using wheat straw as substrate. The BE obtained was 62% and three flushes of mushrooms were harvested. L. tigrinus showed the capacity to degrad...
| Autores: | ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2005 |
| País: | Argentina |
| Institución: | Consejo Nacional de Investigaciones Científicas y Técnicas |
| Repositorio: | CONICET Digital (CONICET) |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:ri.conicet.gov.ar:11336/30489 |
| Acceso en línea: | http://hdl.handle.net/11336/30489 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Cellulases Ligninases Ssf Xylanases White-Rot Fungi https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| _version_ | 1878403115567284224 |
|---|---|
| author | Lechner, Bernardo Ernesto |
| author2 | Papinutti, Víctor Leandro |
| author2_role | author |
| author_browse | Lechner, Bernardo Ernesto Papinutti, Víctor Leandro |
| author_facet | Lechner, Bernardo Ernesto Papinutti, Víctor Leandro |
| author_role | author |
| collection | CONICET Digital (CONICET) |
| description | To investigate the growth and production of lignocellulosic enzymes from Lentinus tigrinus, solid state fermentation (SSF) during 110 days was performed by using wheat straw as substrate. The BE obtained was 62% and three flushes of mushrooms were harvested. L. tigrinus showed the capacity to degrade wheat straw, causing a 21.49% decrease in lignin content and a 53.26% decrease in cellulose. The water extractives (aromatic compounds and reducing sugars) increased to 121.44% along incubation time. All of the enzyme activities were high during colonization and ligninases declined drastically during fruit body formation. After harvesting the last flush, enzyme activities increased rapidly to highest levels, except for laccase activity. Cellulases showed the maximum of activity around 90 days post inoculation. Laccase titres reached a peak of 30 U g−1 at day 20 and MnP had two peaks of around 750 mU g−1 at days 20 and 90. Aromatic compounds from lignin degradation were not inducers of ligninases. Laccase was the most stable enzyme showing complete stability after 42 h at 40 °C. All enzymes showed acid optimum pH. The minor value was recorded for laccase and endoglucanase (pH 3.0). |
| eu_rights_str_mv | openAccess |
| format | article |
| id | AR_4088aa2fc9fa8f4e3755d4904623e596 |
| identifier_str_mv | Lechner, Bernardo Ernesto; Papinutti, Víctor Leandro; Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw; Elsevier; Process Biochemistry; 41; 3; 5-10-2005; 594-598 1359-5113 1873-3298 CONICET Digital CONICET |
| institution | Consejo Nacional de Investigaciones Científicas y Técnicas |
| instname_str | Consejo Nacional de Investigaciones Científicas y Técnicas |
| language | eng |
| network_acronym_str | AR |
| network_name_str | Argentina |
| oai_identifier_str | oai:ri.conicet.gov.ar:11336/30489 |
| publishDate | 2005 |
| publishDateSort | 2005 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | CONICET Digital (CONICET) |
| spelling | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw Lechner, Bernardo Ernesto Papinutti, Víctor Leandro Cellulases Ligninases Ssf Xylanases White-Rot Fungi https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 To investigate the growth and production of lignocellulosic enzymes from Lentinus tigrinus, solid state fermentation (SSF) during 110 days was performed by using wheat straw as substrate. The BE obtained was 62% and three flushes of mushrooms were harvested. L. tigrinus showed the capacity to degrade wheat straw, causing a 21.49% decrease in lignin content and a 53.26% decrease in cellulose. The water extractives (aromatic compounds and reducing sugars) increased to 121.44% along incubation time. All of the enzyme activities were high during colonization and ligninases declined drastically during fruit body formation. After harvesting the last flush, enzyme activities increased rapidly to highest levels, except for laccase activity. Cellulases showed the maximum of activity around 90 days post inoculation. Laccase titres reached a peak of 30 U g−1 at day 20 and MnP had two peaks of around 750 mU g−1 at days 20 and 90. Aromatic compounds from lignin degradation were not inducers of ligninases. Laccase was the most stable enzyme showing complete stability after 42 h at 40 °C. All enzymes showed acid optimum pH. The minor value was recorded for laccase and endoglucanase (pH 3.0). Fil: Lechner, Bernardo Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Fil: Papinutti, Víctor Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina Elsevier http://hdl.handle.net/11336/30489 Lechner, Bernardo Ernesto; Papinutti, Víctor Leandro; Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw; Elsevier; Process Biochemistry; 41; 3; 5-10-2005; 594-598 1359-5113 1873-3298 CONICET Digital CONICET |
| spellingShingle | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw Lechner, Bernardo Ernesto Cellulases Ligninases Ssf Xylanases White-Rot Fungi https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| status_str | publishedVersion |
| title | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw |
| title_full | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw |
| title_fullStr | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw |
| title_full_unstemmed | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw |
| title_short | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw |
| title_sort | Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw |
| topic | Cellulases Ligninases Ssf Xylanases White-Rot Fungi https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| topic_facet | Cellulases Ligninases Ssf Xylanases White-Rot Fungi https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| url | http://hdl.handle.net/11336/30489 |