Molecular modeling and expression analysis of a MADS-box cDNA from mango (Mangifera indica L.)

"MADS-box genes are a large family of transcription factors initially discovered for their role during development of flowers and fruits. The MADS-box transcription factors from animals have been studied by X-ray protein crystallography but those from plants remain to be studied. In this work, a MAD...

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Authors: MAGDA ADELINA PACHECO SANCHEZ; CARMEN ARMINDA CONTRERAS VERGARA; OSCAR EDUARDO HERNANDEZ NAVARRO; GLORIA MARTINA YEPIZ PLASCENCIA; MIGUEL ANGEL MARTINEZ TELLEZ; J SERGIO CASAS FLORES; ALDO ALEJANDRO ARVIZU FLORES; MARIA AUXILIADORA ISLAS OSUNA
Format: Article
Status:Published version
Publication date:2014
Country:México
Institution:IPICYT
Repository:Repositorio Institucional del IPICYT
Language:English
OAI Identifier:oai:ipicyt.repositorioinstitucional.mx:1010/1323
Online Access:http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1323
Access Level:Open access
Keyword:info:eu-repo/classification/Autor/Mangifera indica L.
info:eu-repo/classification/Autor/Gene expression
info:eu-repo/classification/Autor/MADS-box
info:eu-repo/classification/Autor/Molecular modeling
info:eu-repo/classification/Autor/Transcription factor
info:eu-repo/classification/cti/6
Summary:"MADS-box genes are a large family of transcription factors initially discovered for their role during development of flowers and fruits. The MADS-box transcription factors from animals have been studied by X-ray protein crystallography but those from plants remain to be studied. In this work, a MADS-box cDNA from mango encoding a protein of 254 residues was obtained and compared. Based on phylogenetic analysis, it is proposed that the MADS-box transcription factor expressed in mango fruit (MiMADS1) belongs to the SEP clade of MADS-box proteins. MiMADS1 mRNA steady-state levels did not changed during mango fruit development and were up-regulated, when mango fruits reached physiological maturity as assessed by qRT-PCR. Thus, MiMADS1 could have a role during development and ripening of this fruit. The theoretical structural model of MiMADS1 showed the DNA-binding domain folding bound to a double-stranded DNA. Therefore, MiMADS1 is an interesting model for understanding DNA-binding for transcriptional regulation."

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