Effect of alkali treatment time and extraction time on agar from Gracilaria vermiculophylla

The effect of alkali-treatment time and extraction time of native agar and alkali-treated agar obtained from Gracilaria vermiculophylla were studied. The response characteristics were mainly agar yield and gel strength. Alkali-treatment was done for 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 h. Agar yield and...

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Bibliographic Details
Authors: Arvizu Higuera, Dora Luz; Rodríguez Montesinos, Yoloxochilt Elizabeth; Murillob Álvarez, Jesús Iván; Muñoz Ochoa, Mauricio; Hernández Carmona, Gustavo
Format: Article
Status:Published version
Publication date:2008
Country:México
Institution:IPN
Repository:Repositorio Digital del IPN
OAI Identifier:oai:www.repositoriodigital.ipn.mx:123456789/13173
Online Access:http://www.repositoriodigital.ipn.mx/handle/123456789/13173
Access Level:Open access
Keyword:agar yield
Polysaccharide
Gel strength
3-6-anhydrogalactose
Melting and gelling temperatures
Description
Summary:The effect of alkali-treatment time and extraction time of native agar and alkali-treated agar obtained from Gracilaria vermiculophylla were studied. The response characteristics were mainly agar yield and gel strength. Alkali-treatment was done for 0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 h. Agar yield and gel strength decreased with the increase in the time of the alkali-treatment. The highest yield (15.3%) and highest gel strength (1064 g cm-2) were obtained at 0.5 h, therefore this time was used for the next experiment. The extraction of both native agar and alkali-treated agar was done for 1.5, 2.0, 2.5, and 3.0 h. The best extraction time for alkali-treated agar was 1.5 h, and for native agar was 2.5 h. The alkali-treated agar obtained with the different alkali-treatment times and extraction times showed a high melting temperature (92.4 to 99.7 ºC) and gelling temperature (35.7 to 39.6 ºC). Native agar was lower in melting temperature (60.2 to 64.1 ºC) and gelling temperature (20.4 to 23.4 ºC). The 3,6-anhydrogalactose content decreased with increasing alkali-treatment time, with the opposite effect during the extraction of native agar and alkali-treated agar.