Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis
Golgi-specific sialyltransferase (ST) expressed as a chimera with the rapamycin-binding domain of mTOR, FRB, relocates to the endoplasmic reticulum (ER) in cells exposed to rapamycin that also express invariant chain (Ii)-FKBP in the ER. This result has been taken to indicate that Golgi-resident enz...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2017 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/69214 |
| Online Access: | http://hdl.handle.net/10230/69214 http://dx.doi.org/10.1091/mbc.e16-08-0560 |
| Access Level: | Open access |
| Keyword: | Aparell de Golgi Reticle endoplasmàtic Enzims |
| _version_ | 1878742876600401920 |
|---|---|
| author | Villeneuvea, Julien |
| author2 | Duran, Joan Scarpa, Margherita Bassaganyas Bars, Laia, 1985- van Galen, Josse Malhotra, Vivek |
| author2_role | author author author author author |
| author_browse | Bassaganyas Bars, Laia, 1985- Duran, Joan Malhotra, Vivek Scarpa, Margherita Villeneuvea, Julien van Galen, Josse |
| author_facet | Villeneuvea, Julien Duran, Joan Scarpa, Margherita Bassaganyas Bars, Laia, 1985- van Galen, Josse Malhotra, Vivek |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Golgi-specific sialyltransferase (ST) expressed as a chimera with the rapamycin-binding domain of mTOR, FRB, relocates to the endoplasmic reticulum (ER) in cells exposed to rapamycin that also express invariant chain (Ii)-FKBP in the ER. This result has been taken to indicate that Golgi-resident enzymes cycle to the ER constitutively. We show that ST-FRB is trapped in the ER even without Ii-FKBP upon rapamycin addition. This is because ER-Golgi–cycling FKBP proteins contain a C-terminal KDEL-like sequence, bind ST-FRB in the Golgi, and are transported together back to the ER by KDEL receptor–mediated retrograde transport. Moreover, depletion of KDEL receptor prevents trapping of ST-FRB in the ER by rapamycin. Thus ST-FRB cycles artificially by binding to FKBP domain–containing proteins. In addition, Golgi-specific O-linked glycosylation of a resident ER protein occurs only upon artificial fusion of Golgi membranes with ER. Together these findings support the consensus view that there is no appreciable mixing of Golgi-resident enzymes with ER under normal conditions. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_fdd77f87c5f7ad603a85625d272e22f6 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:10230/69214 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | American Society for Cell Biology |
| publisherStr | American Society for Cell Biology |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis Villeneuvea, Julien Duran, Joan Scarpa, Margherita Bassaganyas Bars, Laia, 1985- van Galen, Josse Malhotra, Vivek Aparell de Golgi Reticle endoplasmàtic Enzims Golgi-specific sialyltransferase (ST) expressed as a chimera with the rapamycin-binding domain of mTOR, FRB, relocates to the endoplasmic reticulum (ER) in cells exposed to rapamycin that also express invariant chain (Ii)-FKBP in the ER. This result has been taken to indicate that Golgi-resident enzymes cycle to the ER constitutively. We show that ST-FRB is trapped in the ER even without Ii-FKBP upon rapamycin addition. This is because ER-Golgi–cycling FKBP proteins contain a C-terminal KDEL-like sequence, bind ST-FRB in the Golgi, and are transported together back to the ER by KDEL receptor–mediated retrograde transport. Moreover, depletion of KDEL receptor prevents trapping of ST-FRB in the ER by rapamycin. Thus ST-FRB cycles artificially by binding to FKBP domain–containing proteins. In addition, Golgi-specific O-linked glycosylation of a resident ER protein occurs only upon artificial fusion of Golgi membranes with ER. Together these findings support the consensus view that there is no appreciable mixing of Golgi-resident enzymes with ER under normal conditions. We thank all members of the Malhotra laboratory for valuable discussions and Arrate Mallabiabarrena and Raquel García for help with fluorescence microscopy. J.V. acknowledges support from a Marie Curie International Outgoing Fellowship within the 7th European Community Framework Program. V.M. is an Institució Catalana de Recerca i Estudis Avançats Professor at the Center for Genomic Regulation, and the work in his laboratory is funded by grants from Plan Nacional (BFU2008-00414), Consolider (CSD2009-00016), the Agència de Gestió d’Ajuts Universitaris i de Recerca Grups de Recerca Emergents (SGR2009-1488; AGAUR-Catalan Government), and the European Research Council (268692). The project received research funding from the European Union. We acknowledge support of the Spanish Ministry of Economy and Competitiveness, Centro de Excelencia Severo Ochoa 2013-2017. This article reflects the views of only the authors. The European Union is not liable for any use that may be made of the information contained herein. American Society for Cell Biology http://hdl.handle.net/10230/69214 http://dx.doi.org/10.1091/mbc.e16-08-0560 |
| spellingShingle | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis Villeneuvea, Julien Aparell de Golgi Reticle endoplasmàtic Enzims |
| status_str | publishedVersion |
| title | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis |
| title_full | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis |
| title_fullStr | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis |
| title_full_unstemmed | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis |
| title_short | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis |
| title_sort | Golgi enzymes do not cycle through the endoplasmic reticulum during protein secretion or mitosis |
| topic | Aparell de Golgi Reticle endoplasmàtic Enzims |
| topic_facet | Aparell de Golgi Reticle endoplasmàtic Enzims |
| url | http://hdl.handle.net/10230/69214 http://dx.doi.org/10.1091/mbc.e16-08-0560 |