Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms
In biosensor development, silk fibroin is advantageous for providing transparent, flexible, chemically/mechanically stable, biocompatible, and sustainable substrates, where the biorecognition element remains functional for long time periods. These properties are employed here in the production of po...
| Autores: | ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:ddd.uab.cat:289367 |
| Acceso en línea: | https://ddd.uab.cat/record/289367 https://dx.doi.org/urn:doi:10.1021/acsabm.3c00872 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Silk fibroin Colorimetric biosensor Photoelectrochromic Dithienylethene mediators Photopatterning Point of care Glucose detection |
| _version_ | 1878742688954580992 |
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| author | Márquez, Augusto|||0000-0003-3074-7941 |
| author2 | Santiago Malagon, Sara|||0000-0002-1206-1455 Santos, Molíria V.|||0000-0003-0569-2424 Aznar-Cervantes, Salvador|||0000-0001-9809-7215 Dominguez, Carlos|||0000-0002-5972-7285 Omenetto, Fiorenzo|||0000-0002-0327-853X Guirado, Gonzalo|||0000-0003-2128-7007 Muñoz-Berbel, Xavier|||0000-0002-6447-5756 |
| author2_role | author author author author author author author |
| author_browse | Aznar-Cervantes, Salvador|||0000-0001-9809-7215 Dominguez, Carlos|||0000-0002-5972-7285 Guirado, Gonzalo|||0000-0003-2128-7007 Muñoz-Berbel, Xavier|||0000-0002-6447-5756 Márquez, Augusto|||0000-0003-3074-7941 Omenetto, Fiorenzo|||0000-0002-0327-853X Santiago Malagon, Sara|||0000-0002-1206-1455 Santos, Molíria V.|||0000-0003-0569-2424 |
| author_facet | Márquez, Augusto|||0000-0003-3074-7941 Santiago Malagon, Sara|||0000-0002-1206-1455 Santos, Molíria V.|||0000-0003-0569-2424 Aznar-Cervantes, Salvador|||0000-0001-9809-7215 Dominguez, Carlos|||0000-0002-5972-7285 Omenetto, Fiorenzo|||0000-0002-0327-853X Guirado, Gonzalo|||0000-0003-2128-7007 Muñoz-Berbel, Xavier|||0000-0002-6447-5756 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | In biosensor development, silk fibroin is advantageous for providing transparent, flexible, chemically/mechanically stable, biocompatible, and sustainable substrates, where the biorecognition element remains functional for long time periods. These properties are employed here in the production of point-of-care biosensors for resource-limited regions, which are able to display glucose levels without the need for external instrumentation. These biosensors are produced by photopatterning silk films doped with the enzymes glucose oxidase and peroxidase and photoelectrochromic molecules from the dithienylethene family acting as colorimetric mediators of the enzymatic reaction. The photopatterning results from the photoisomerization of dithienylethene molecules in the silk film from its initial uncolored opened form to its pink closed one. The photoisomerization is dose-dependent, and colored patterns with increasing color intensities are obtained by increasing either the irradiation time or the light intensity. In the presence of glucose, the enzymatic cascade reaction is activated, and peroxidase selectively returns closed dithienylethene molecules to their initial uncolored state. Color disappearance in the silk film is proportional to glucose concentration and used to distinguish between hypoglycemic (below 4 mM), normoglycemic (4-6 mM), and hyperglycemic levels (above 6 mM) by visual inspection. After the measurement, the biosensor can be regenerated by irradiation with UV light, enabling up to five measurement cycles. The coupling of peroxidase activity to other oxidoreductases opens the possibility to produce long-life reusable smart biosensors for other analytes such as lactate, cholesterol, or ethanol. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_fa783497d0e94db76a59ae996e8fd669 |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:289367 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms Márquez, Augusto|||0000-0003-3074-7941 Santiago Malagon, Sara|||0000-0002-1206-1455 Santos, Molíria V.|||0000-0003-0569-2424 Aznar-Cervantes, Salvador|||0000-0001-9809-7215 Dominguez, Carlos|||0000-0002-5972-7285 Omenetto, Fiorenzo|||0000-0002-0327-853X Guirado, Gonzalo|||0000-0003-2128-7007 Muñoz-Berbel, Xavier|||0000-0002-6447-5756 Silk fibroin Colorimetric biosensor Photoelectrochromic Dithienylethene mediators Photopatterning Point of care Glucose detection In biosensor development, silk fibroin is advantageous for providing transparent, flexible, chemically/mechanically stable, biocompatible, and sustainable substrates, where the biorecognition element remains functional for long time periods. These properties are employed here in the production of point-of-care biosensors for resource-limited regions, which are able to display glucose levels without the need for external instrumentation. These biosensors are produced by photopatterning silk films doped with the enzymes glucose oxidase and peroxidase and photoelectrochromic molecules from the dithienylethene family acting as colorimetric mediators of the enzymatic reaction. The photopatterning results from the photoisomerization of dithienylethene molecules in the silk film from its initial uncolored opened form to its pink closed one. The photoisomerization is dose-dependent, and colored patterns with increasing color intensities are obtained by increasing either the irradiation time or the light intensity. In the presence of glucose, the enzymatic cascade reaction is activated, and peroxidase selectively returns closed dithienylethene molecules to their initial uncolored state. Color disappearance in the silk film is proportional to glucose concentration and used to distinguish between hypoglycemic (below 4 mM), normoglycemic (4-6 mM), and hyperglycemic levels (above 6 mM) by visual inspection. After the measurement, the biosensor can be regenerated by irradiation with UV light, enabling up to five measurement cycles. The coupling of peroxidase activity to other oxidoreductases opens the possibility to produce long-life reusable smart biosensors for other analytes such as lactate, cholesterol, or ethanol. https://ddd.uab.cat/record/289367 https://dx.doi.org/urn:doi:10.1021/acsabm.3c00872 |
| spellingShingle | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms Márquez, Augusto|||0000-0003-3074-7941 Silk fibroin Colorimetric biosensor Photoelectrochromic Dithienylethene mediators Photopatterning Point of care Glucose detection |
| title | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms |
| title_full | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms |
| title_fullStr | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms |
| title_full_unstemmed | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms |
| title_short | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms |
| title_sort | Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms |
| topic | Silk fibroin Colorimetric biosensor Photoelectrochromic Dithienylethene mediators Photopatterning Point of care Glucose detection |
| topic_facet | Silk fibroin Colorimetric biosensor Photoelectrochromic Dithienylethene mediators Photopatterning Point of care Glucose detection |
| url | https://ddd.uab.cat/record/289367 https://dx.doi.org/urn:doi:10.1021/acsabm.3c00872 |