Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS
Objectives: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine t...
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL) |
| Repositorio: | r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante |
| OAI Identifier: | oai:isabial.fundanetsuite.com:p5883 |
| Acceso en línea: | https://isabial.portalinvestigacion.com/publicaciones5883 |
| Nivel de acceso: | Acceso abierto |
| _version_ | 1878742167089840128 |
|---|---|
| author | Perez-Vazquez, M |
| author2 | Campoy, PJS Ortega, A Bautista, V Monzon, S Ruiz-Carrascoso, G Mingorance, J Gonzalez-Barbera, EM Gimeno, C Aracil, B Saez, D Lara, N Fernandez, S Gonzalez-Lopez, JJ Campos, J Kingsley, RA Dougan, G Oteo-Iglesias, J Rodrigo, CH Aznar, E Garcia-Picazo, L Martinez, BO Mansilla, EC Alvarez-Garcia, P Carretero, JMP Rodriguez-Conde, I Cao, SMR Gimeno, A Moreno, MOP Rodriguez, MLC Cavero, TA Gil, Y Esteras, MAR Andres, JLB Trujillo, G Almaraz, JLH Leon, JL del Pozo, JL Salas, CM Adan, FB Bertomeu, FG Cascales, P Dorado, JC Suarez, CB Spanish NDM Study Grp |
| author2_role | author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
| author_browse | Adan, FB Almaraz, JLH Alvarez-Garcia, P Andres, JLB Aracil, B Aznar, E Bautista, V Bertomeu, FG Campos, J Campoy, PJS Cao, SMR Carretero, JMP Cascales, P Cavero, TA Dorado, JC Dougan, G Esteras, MAR Fernandez, S Garcia-Picazo, L Gil, Y Gimeno, A Gimeno, C Gonzalez-Barbera, EM Gonzalez-Lopez, JJ Kingsley, RA Lara, N Leon, JL Mansilla, EC Martinez, BO Mingorance, J Monzon, S Moreno, MOP Ortega, A Oteo-Iglesias, J Perez-Vazquez, M Rodrigo, CH Rodriguez, MLC Rodriguez-Conde, I Ruiz-Carrascoso, G Saez, D Salas, CM Spanish NDM Study Grp Suarez, CB Trujillo, G del Pozo, JL |
| author_facet | Perez-Vazquez, M Campoy, PJS Ortega, A Bautista, V Monzon, S Ruiz-Carrascoso, G Mingorance, J Gonzalez-Barbera, EM Gimeno, C Aracil, B Saez, D Lara, N Fernandez, S Gonzalez-Lopez, JJ Campos, J Kingsley, RA Dougan, G Oteo-Iglesias, J Rodrigo, CH Aznar, E Garcia-Picazo, L Martinez, BO Mansilla, EC Alvarez-Garcia, P Carretero, JMP Rodriguez-Conde, I Cao, SMR Gimeno, A Moreno, MOP Rodriguez, MLC Cavero, TA Gil, Y Esteras, MAR Andres, JLB Trujillo, G Almaraz, JLH Leon, JL del Pozo, JL Salas, CM Adan, FB Bertomeu, FG Cascales, P Dorado, JC Suarez, CB Spanish NDM Study Grp |
| author_role | author |
| collection | r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante |
| description | Objectives: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine the plasmids harbouring bla(NDM)-like genes. Methods: High-resolution SNP typing, core-genome MLST and plasmid reconstruction (PlasmidID) were performed on 59 NDM-like-producing K. pneumoniae and 8 NDM-like-producing E. coli isolated over an 8year period in Spain. Results: Five major epidemic clones of NDM-producing K. pneumoniae caused five important nationwide outbreaks: ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1; in contrast, the spread of NDM-producing E. coli was polyclonal. Three bla(NDM) types were identified: bla(NDM-1), 61.2%; bla(NDM-7), 32.8%; and bla(NDM-5), 6%. Five K. pneumoniae isolates co-produced other carbapenemases (three bla(OXA-48) and two bla(VIM-1)). The average number of acquired resistance genes was higher in K. pneumoniae than in E. coli. The plasmids encoding bla(NDM)-like genes belonged to IncFII, IncFIB, IncX3, IncR, IncN and IncC types, of which IncF, IncR and IncC were associated with MDR. The genetic surroundings of bla(NDM)-like genes showed a highly variable region upstream of ISAba125. Conclusions: In recent years NDM-producing K. pneumoniae and E. coli have emerged in Spain; the spread of a few high-risk K. pneumoniae clones such as ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1 have caused several interregional outbreaks. In contrast, the spread of NDM-producing E. coli has been polyclonal. Plasmid types IncFII, IncFIB, IncX3, IncR, IncN and IncC carried bla(NDM), and the same IncX3 plasmid was detected in K. pneumoniae and E. coli. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_f13bd85492e4b1b7daee5f42190cbe9d |
| institution | Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL) |
| instname_str | Instituto de Investigación Biomédica y Sanitaria de Alicante (ISABIAL) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:isabial.fundanetsuite.com:p5883 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| publisher | OXFORD UNIV PRESS |
| publisherStr | OXFORD UNIV PRESS |
| reponame_str | r-ISABIAL. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica y Sanitaria de Alicante |
| spelling | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS Perez-Vazquez, M Campoy, PJS Ortega, A Bautista, V Monzon, S Ruiz-Carrascoso, G Mingorance, J Gonzalez-Barbera, EM Gimeno, C Aracil, B Saez, D Lara, N Fernandez, S Gonzalez-Lopez, JJ Campos, J Kingsley, RA Dougan, G Oteo-Iglesias, J Rodrigo, CH Aznar, E Garcia-Picazo, L Martinez, BO Mansilla, EC Alvarez-Garcia, P Carretero, JMP Rodriguez-Conde, I Cao, SMR Gimeno, A Moreno, MOP Rodriguez, MLC Cavero, TA Gil, Y Esteras, MAR Andres, JLB Trujillo, G Almaraz, JLH Leon, JL del Pozo, JL Salas, CM Adan, FB Bertomeu, FG Cascales, P Dorado, JC Suarez, CB Spanish NDM Study Grp Objectives: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine the plasmids harbouring bla(NDM)-like genes. Methods: High-resolution SNP typing, core-genome MLST and plasmid reconstruction (PlasmidID) were performed on 59 NDM-like-producing K. pneumoniae and 8 NDM-like-producing E. coli isolated over an 8year period in Spain. Results: Five major epidemic clones of NDM-producing K. pneumoniae caused five important nationwide outbreaks: ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1; in contrast, the spread of NDM-producing E. coli was polyclonal. Three bla(NDM) types were identified: bla(NDM-1), 61.2%; bla(NDM-7), 32.8%; and bla(NDM-5), 6%. Five K. pneumoniae isolates co-produced other carbapenemases (three bla(OXA-48) and two bla(VIM-1)). The average number of acquired resistance genes was higher in K. pneumoniae than in E. coli. The plasmids encoding bla(NDM)-like genes belonged to IncFII, IncFIB, IncX3, IncR, IncN and IncC types, of which IncF, IncR and IncC were associated with MDR. The genetic surroundings of bla(NDM)-like genes showed a highly variable region upstream of ISAba125. Conclusions: In recent years NDM-producing K. pneumoniae and E. coli have emerged in Spain; the spread of a few high-risk K. pneumoniae clones such as ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1 have caused several interregional outbreaks. In contrast, the spread of NDM-producing E. coli has been polyclonal. Plasmid types IncFII, IncFIB, IncX3, IncR, IncN and IncC carried bla(NDM), and the same IncX3 plasmid was detected in K. pneumoniae and E. coli. OXFORD UNIV PRESS https://isabial.portalinvestigacion.com/publicaciones5883 |
| spellingShingle | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS Perez-Vazquez, M |
| status_str | publishedVersion |
| title | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS |
| title_full | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS |
| title_fullStr | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS |
| title_full_unstemmed | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS |
| title_short | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS |
| title_sort | Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding bla(NDM)-like genes as determined by WGS |
| url | https://isabial.portalinvestigacion.com/publicaciones5883 |