Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis
Alzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of Aβ(1...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2025 |
| Country: | España |
| Institution: | Universidad de Santiago de Compostela (USC) |
| Repository: | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| Language: | English |
| OAI Identifier: | oai:minerva.usc.gal:10347/43532 |
| Online Access: | https://hdl.handle.net/10347/43532 |
| Access Level: | Open access |
| Keyword: | Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| _version_ | 1878742113080836096 |
|---|---|
| author | Novo, Mercedes |
| author2 | Illodo Brea, Sara Seijas Cerceda, Jesús Moraes, Stella Hernández Faria De Rodríguez-Prieto, Flor Al-Soufi, Wajih |
| author2_role | author author author author author |
| author_browse | Al-Soufi, Wajih Illodo Brea, Sara Moraes, Stella Hernández Faria De Novo, Mercedes Rodríguez-Prieto, Flor Seijas Cerceda, Jesús |
| author_facet | Novo, Mercedes Illodo Brea, Sara Seijas Cerceda, Jesús Moraes, Stella Hernández Faria De Rodríguez-Prieto, Flor Al-Soufi, Wajih |
| author_role | author |
| collection | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| description | Alzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of Aβ(1–40) (Aβ40) as a label-free approach to detecting early-stage oligomers. Aβ40 exhibits autofluorescence dominated by tyrosine emission, which undergoes a strong blue shift and quenching during oligomerization. Additionally, aggregation-induced emission (AIE) in the visible spectral region emerges, correlating with Aβ oligomer concentration and providing a means to detect and quantify oligomers. At the critical aggregation concentrations cac1 = 0.5 μM and cac2 = 19 μM, distinct aggregation behaviours are observed. By employing steady-state fluorescence spectroscopy, a widely accessible technique, these findings establish a direct link between early Aβ aggregation and intrinsic fluorescence changes. This approach eliminates the need for extrinsic probes, simplifying experimental procedures and reducing artefacts. Although further studies are required to develop a robust quantitative correlation for potential diagnostic applications, Aβ autofluorescence represents a promising strategy for investigating early aggregation processes in the context of AD |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_f04f4dfb3aa9e1aa6dc35ebbfaebca0b |
| institution | Universidad de Santiago de Compostela (USC) |
| instname_str | Universidad de Santiago de Compostela (USC) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:minerva.usc.gal:10347/43532 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| publisher | Royal society of chemistry |
| publisherStr | Royal society of chemistry |
| reponame_str | Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela |
| spelling | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis Novo, Mercedes Illodo Brea, Sara Seijas Cerceda, Jesús Moraes, Stella Hernández Faria De Rodríguez-Prieto, Flor Al-Soufi, Wajih Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis Alzheimer's disease (AD) is a major public health challenge, with its onset occurring years before symptoms appear. Soluble amyloid-β (Aβ) oligomers are key species in AD pathogenesis and diagnosis, highlighting the need for early detection. This study investigates the intrinsic fluorescence of Aβ(1–40) (Aβ40) as a label-free approach to detecting early-stage oligomers. Aβ40 exhibits autofluorescence dominated by tyrosine emission, which undergoes a strong blue shift and quenching during oligomerization. Additionally, aggregation-induced emission (AIE) in the visible spectral region emerges, correlating with Aβ oligomer concentration and providing a means to detect and quantify oligomers. At the critical aggregation concentrations cac1 = 0.5 μM and cac2 = 19 μM, distinct aggregation behaviours are observed. By employing steady-state fluorescence spectroscopy, a widely accessible technique, these findings establish a direct link between early Aβ aggregation and intrinsic fluorescence changes. This approach eliminates the need for extrinsic probes, simplifying experimental procedures and reducing artefacts. Although further studies are required to develop a robust quantitative correlation for potential diagnostic applications, Aβ autofluorescence represents a promising strategy for investigating early aggregation processes in the context of AD Royal society of chemistry https://hdl.handle.net/10347/43532 |
| spellingShingle | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis Novo, Mercedes Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| title | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_full | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_fullStr | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_full_unstemmed | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_short | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| title_sort | Intrinsic visible emission of amyloid-β oligomers: a potential tool for early alzheimer's diagnosis |
| topic | Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| topic_facet | Alzheimer’s disease Soluble amyloid-b (Ab) oligomers Pathogenesis diagnosis |
| url | https://hdl.handle.net/10347/43532 |