Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients
Diabetic patients present increased volume and functional alterations in epicardial adipose tissue (EAT). We aimed to analyze EAT from type 2 diabetic patients and the inflammatory and cytotoxic effects induced on cardiomyocytes. Furthermore, we analyzed the cardioprotective role of apolipoprotein J...
| Authors: | ; ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:306970 |
| Online Access: | https://ddd.uab.cat/record/306970 https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779 |
| Access Level: | Open access |
| Keyword: | Apolipoprotein J Apoptosis Ceramides Coronary artery disease Epicardial adipose tissue Fatty acids Inflammation Type 2 diabetes |
| _version_ | 1878742081726316544 |
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| author | Puig Grifol, Núria|||0000-0002-4699-3162 |
| author2 | Rives Jimenez, Jose|||0009-0001-5725-9270 Gil-Millan, Pedro Miñambres, Inka|||0000-0002-0899-3938 Ginel, Antonino|||0000-0002-8820-7545 Tauron, Manel|||0000-0002-5864-519X Bonaterra-Pastra, Anna|||0000-0002-1480-622X Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091 Pérez, Antonio|||0000-0001-5528-1143 Sánchez Quesada, José Luis|||0000-0003-0224-591X Benitez, Sonia|||0000-0003-3565-0743 |
| author2_role | author author author author author author author author author author |
| author_browse | Benitez, Sonia|||0000-0003-3565-0743 Bonaterra-Pastra, Anna|||0000-0002-1480-622X Gil-Millan, Pedro Ginel, Antonino|||0000-0002-8820-7545 Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091 Miñambres, Inka|||0000-0002-0899-3938 Puig Grifol, Núria|||0000-0002-4699-3162 Pérez, Antonio|||0000-0001-5528-1143 Rives Jimenez, Jose|||0009-0001-5725-9270 Sánchez Quesada, José Luis|||0000-0003-0224-591X Tauron, Manel|||0000-0002-5864-519X |
| author_facet | Puig Grifol, Núria|||0000-0002-4699-3162 Rives Jimenez, Jose|||0009-0001-5725-9270 Gil-Millan, Pedro Miñambres, Inka|||0000-0002-0899-3938 Ginel, Antonino|||0000-0002-8820-7545 Tauron, Manel|||0000-0002-5864-519X Bonaterra-Pastra, Anna|||0000-0002-1480-622X Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091 Pérez, Antonio|||0000-0001-5528-1143 Sánchez Quesada, José Luis|||0000-0003-0224-591X Benitez, Sonia|||0000-0003-3565-0743 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Diabetic patients present increased volume and functional alterations in epicardial adipose tissue (EAT). We aimed to analyze EAT from type 2 diabetic patients and the inflammatory and cytotoxic effects induced on cardiomyocytes. Furthermore, we analyzed the cardioprotective role of apolipoprotein J (apoJ). EAT explants were obtained from nondiabetic patients (ND), diabetic patients without coronary disease (DM), and DM patients with coronary disease (DM-C) after heart surgery. Morphological characteristics and gene expression were evaluated. Explants were cultured for 24 h and the content of nonesterified fatty acids (NEFA) and sphingolipid species in secretomes was evaluated by lipidomic analysis. Afterwards, secretomes were added to AC16 human cardiomyocytes for 24 h in the presence or absence of cardioprotective molecules (apoJ and HDL). Cytokine release and apoptosis/necrosis were assessed by ELISA and flow cytometry. The EAT from the diabetic samples showed altered expression of genes related to lipid accumulation, insulin resistance, and inflammation. The secretomes from the DM samples presented an increased ratio of pro/antiatherogenic ceramide (Cer) species, while those from DM-C contained the highest concentration of saturated NEFA. DM and DM-C secretomes promoted inflammation and cytotoxicity on AC16 cardiomyocytes. Exogenous Cer16:0, Cer24:1, and palmitic acid reproduced deleterious effects in AC16 cells. These effects were attenuated by exogenous apoJ. Diabetic secretomes promoted inflammation and cytotoxicity in cardiomyocytes. This effect was exacerbated in the secretomes of the DM-C samples. The increased content of specific NEFA and ceramide species seems to play a key role in inducing such deleterious effects, which are attenuated by apoJ. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_efd3ec98e768dfe4e897b04ba32c9ef2 |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:306970 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients Puig Grifol, Núria|||0000-0002-4699-3162 Rives Jimenez, Jose|||0009-0001-5725-9270 Gil-Millan, Pedro Miñambres, Inka|||0000-0002-0899-3938 Ginel, Antonino|||0000-0002-8820-7545 Tauron, Manel|||0000-0002-5864-519X Bonaterra-Pastra, Anna|||0000-0002-1480-622X Hernandez Guillamon, Maria Mar|||0000-0001-8844-0091 Pérez, Antonio|||0000-0001-5528-1143 Sánchez Quesada, José Luis|||0000-0003-0224-591X Benitez, Sonia|||0000-0003-3565-0743 Apolipoprotein J Apoptosis Ceramides Coronary artery disease Epicardial adipose tissue Fatty acids Inflammation Type 2 diabetes Diabetic patients present increased volume and functional alterations in epicardial adipose tissue (EAT). We aimed to analyze EAT from type 2 diabetic patients and the inflammatory and cytotoxic effects induced on cardiomyocytes. Furthermore, we analyzed the cardioprotective role of apolipoprotein J (apoJ). EAT explants were obtained from nondiabetic patients (ND), diabetic patients without coronary disease (DM), and DM patients with coronary disease (DM-C) after heart surgery. Morphological characteristics and gene expression were evaluated. Explants were cultured for 24 h and the content of nonesterified fatty acids (NEFA) and sphingolipid species in secretomes was evaluated by lipidomic analysis. Afterwards, secretomes were added to AC16 human cardiomyocytes for 24 h in the presence or absence of cardioprotective molecules (apoJ and HDL). Cytokine release and apoptosis/necrosis were assessed by ELISA and flow cytometry. The EAT from the diabetic samples showed altered expression of genes related to lipid accumulation, insulin resistance, and inflammation. The secretomes from the DM samples presented an increased ratio of pro/antiatherogenic ceramide (Cer) species, while those from DM-C contained the highest concentration of saturated NEFA. DM and DM-C secretomes promoted inflammation and cytotoxicity on AC16 cardiomyocytes. Exogenous Cer16:0, Cer24:1, and palmitic acid reproduced deleterious effects in AC16 cells. These effects were attenuated by exogenous apoJ. Diabetic secretomes promoted inflammation and cytotoxicity in cardiomyocytes. This effect was exacerbated in the secretomes of the DM-C samples. The increased content of specific NEFA and ceramide species seems to play a key role in inducing such deleterious effects, which are attenuated by apoJ. https://ddd.uab.cat/record/306970 https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779 |
| spellingShingle | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients Puig Grifol, Núria|||0000-0002-4699-3162 Apolipoprotein J Apoptosis Ceramides Coronary artery disease Epicardial adipose tissue Fatty acids Inflammation Type 2 diabetes |
| title | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients |
| title_full | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients |
| title_fullStr | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients |
| title_full_unstemmed | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients |
| title_short | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients |
| title_sort | Apolipoprotein J protects cardiomyocytes from lipid-mediated inflammation and cytotoxicity induced by the epicardial adipose tissue of diabetic patients |
| topic | Apolipoprotein J Apoptosis Ceramides Coronary artery disease Epicardial adipose tissue Fatty acids Inflammation Type 2 diabetes |
| topic_facet | Apolipoprotein J Apoptosis Ceramides Coronary artery disease Epicardial adipose tissue Fatty acids Inflammation Type 2 diabetes |
| url | https://ddd.uab.cat/record/306970 https://dx.doi.org/urn:doi:10.1016/j.biopha.2024.116779 |