Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO)
Enhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate...
| Authors: | ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Accepted version |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/218415 |
| Online Access: | https://hdl.handle.net/2445/218415 |
| Access Level: | Open access |
| Keyword: | Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution |
| _version_ | 1878742010785955840 |
|---|---|
| author | Gil-Villalba, Sergio |
| author2 | Palau, Jordi Soder-Walz, Jesica M. Vallecillo, Miguel A. Corregidor, Jordi Tirado, Andrea Shouakar-Stash, O. Guivernau, Miriam Viñas, Miquel Soler, Anna Rosell, Mònica |
| author2_role | author author author author author author author author author author |
| author_browse | Corregidor, Jordi Gil-Villalba, Sergio Guivernau, Miriam Palau, Jordi Rosell, Mònica Shouakar-Stash, O. Soder-Walz, Jesica M. Soler, Anna Tirado, Andrea Vallecillo, Miguel A. Viñas, Miquel |
| author_facet | Gil-Villalba, Sergio Palau, Jordi Soder-Walz, Jesica M. Vallecillo, Miguel A. Corregidor, Jordi Tirado, Andrea Shouakar-Stash, O. Guivernau, Miriam Viñas, Miquel Soler, Anna Rosell, Mònica |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Enhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate the CEs (PCE, TCE and cis-DCE) bioremediation using EVO in a contaminated site. A multiple approach using C and Cl-CSIA, quantification of Dehalococcoides (Dhc) and specific reductive dechlorination (RD) gene population, and hydrochemical data in microcosm experiments and field samples was applied. Despite the high partitioning of CEs into the EVO phase, the carbon isotopic values of the remaining CEs fraction in the aqueous phase did not exhibit significant changes caused by phase partitioning in laboratory experiments. Both microcosm experiments and field data revealed a rapid RD of PCE and TCE, resulting in the transient accumulation of cis-DCE, which was slowly degraded to vinyl chloride (VC). These results agreed with the presence of Dhc populations and a shift to stronger reducing conditions in the field: i) RD functional genes (tceA, vcrA and bvcA) exhibited a trend to higher values and ii) a substantial increase in Dhc populations (up to 30% of the total bacterial populations) was observed over time. The dual-element isotope slope ΛC-Cl for RD of cis-DCE obtained from field data (ΛC – Cl = 5 ± 3) was similar to the one determined from the microcosm experiments under controlled anoxic conditions (ΛC – Cl = 4.9 ± 0.8). However, ΛC-Cl values differ from those reported so far for laboratory studies with Dhc strains and mixed cultures containing Dhc, i.e., between 8.3 and 17.8. This observation underscores the potential variety of reductive dehalogenases involved during cis-DCE RD and the importance of determining site-specific Λ and ε values in order to improve the identification and quantification of transformation processes in the field. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_eebde7fdf5dc3decd0bcb8cd8b56f10a |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/218415 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier B.V. |
| publisherStr | Elsevier B.V. |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) Gil-Villalba, Sergio Palau, Jordi Soder-Walz, Jesica M. Vallecillo, Miguel A. Corregidor, Jordi Tirado, Andrea Shouakar-Stash, O. Guivernau, Miriam Viñas, Miquel Soler, Anna Rosell, Mònica Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution Enhanced In Situ Bioremediation (EISB) using Emulsified Vegetable Oil (EVO) as a long-term electron donor has gained prominence for the treatment of groundwater contaminated with chlorinated ethenes (CEs). This study explores the potential of isotopic and molecular biology tools (MBT) to investigate the CEs (PCE, TCE and cis-DCE) bioremediation using EVO in a contaminated site. A multiple approach using C and Cl-CSIA, quantification of Dehalococcoides (Dhc) and specific reductive dechlorination (RD) gene population, and hydrochemical data in microcosm experiments and field samples was applied. Despite the high partitioning of CEs into the EVO phase, the carbon isotopic values of the remaining CEs fraction in the aqueous phase did not exhibit significant changes caused by phase partitioning in laboratory experiments. Both microcosm experiments and field data revealed a rapid RD of PCE and TCE, resulting in the transient accumulation of cis-DCE, which was slowly degraded to vinyl chloride (VC). These results agreed with the presence of Dhc populations and a shift to stronger reducing conditions in the field: i) RD functional genes (tceA, vcrA and bvcA) exhibited a trend to higher values and ii) a substantial increase in Dhc populations (up to 30% of the total bacterial populations) was observed over time. The dual-element isotope slope ΛC-Cl for RD of cis-DCE obtained from field data (ΛC – Cl = 5 ± 3) was similar to the one determined from the microcosm experiments under controlled anoxic conditions (ΛC – Cl = 4.9 ± 0.8). However, ΛC-Cl values differ from those reported so far for laboratory studies with Dhc strains and mixed cultures containing Dhc, i.e., between 8.3 and 17.8. This observation underscores the potential variety of reductive dehalogenases involved during cis-DCE RD and the importance of determining site-specific Λ and ε values in order to improve the identification and quantification of transformation processes in the field. Elsevier B.V. https://hdl.handle.net/2445/218415 |
| spellingShingle | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) Gil-Villalba, Sergio Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution |
| status_str | acceptedVersion |
| title | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) |
| title_full | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) |
| title_fullStr | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) |
| title_full_unstemmed | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) |
| title_short | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) |
| title_sort | Use of isotopic (C, Cl) and molecular biology tools to assess biodegradation in a source area of chlorinated ethenes after biostimulation with Emulsified Vegetable Oil (EVO) |
| topic | Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution |
| topic_facet | Bioremediació Depuració d'aigües residuals Isòtops Contaminació de l'aigua Bioremediation Purification of sewage Isotopes Water pollution |
| url | https://hdl.handle.net/2445/218415 |