Identification of zoonotic genotypes of Giardia duodenalis
Giardia duodenalis, originally regarded as a commensal organism, is the etiologic agent of giardiasis, a gastrointestinal disease of humans and animals. Giardiasis causes major public and veterinary health concerns worldwide. Transmission is either direct, through the faecal-oral route, or indirect,...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2009 |
| Country: | España |
| Institution: | Instituto de Salud Carlos III (ISCIII) |
| Repository: | Repisalud |
| Language: | English |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/16292 |
| Online Access: | http://hdl.handle.net/20.500.12105/16292 |
| Access Level: | Open access |
| Keyword: | Animals Cats Cattle DNA, Protozoan Dogs Genetic Variation Genotype Giardia lamblia Giardiasis Goats Humans Molecular Sequence Data Phylogeny Sheep Swine Zoonoses |
| _version_ | 1878741871640969216 |
|---|---|
| author | Sprong, Hein |
| author2 | Cacciò, Simone M van der Giessen, Joke W B ZOOPNET network and partners Rubio Muñoz, Jose Miguel Fuentes Corripio, Isabel |
| author2_role | author author author author author |
| author_browse | Cacciò, Simone M Fuentes Corripio, Isabel Rubio Muñoz, Jose Miguel Sprong, Hein ZOOPNET network and partners van der Giessen, Joke W B |
| author_facet | Sprong, Hein Cacciò, Simone M van der Giessen, Joke W B ZOOPNET network and partners Rubio Muñoz, Jose Miguel Fuentes Corripio, Isabel |
| author_role | author |
| collection | Repisalud |
| description | Giardia duodenalis, originally regarded as a commensal organism, is the etiologic agent of giardiasis, a gastrointestinal disease of humans and animals. Giardiasis causes major public and veterinary health concerns worldwide. Transmission is either direct, through the faecal-oral route, or indirect, through ingestion of contaminated water or food. Genetic characterization of G. duodenalis isolates has revealed the existence of seven groups (assemblages A to G) which differ in their host distribution. Assemblages A and B are found in humans and in many other mammals, but the role of animals in the epidemiology of human infection is still unclear, despite the fact that the zoonotic potential of Giardia was recognised by the WHO some 30 years ago. Here, we performed an extensive genetic characterization of 978 human and 1440 animal isolates, which together comprise 3886 sequences from 4 genetic loci. The data were assembled into a molecular epidemiological database developed by a European network of public and veterinary health Institutions. Genotyping was performed at different levels of resolution (single and multiple loci on the same dataset). The zoonotic potential of both assemblages A and B is evident when studied at the level of assemblages, sub-assemblages, and even at each single locus. However, when genotypes are defined using a multi-locus sequence typing scheme, only 2 multi-locus genotypes (MLG) of assemblage A and none of assemblage B appear to have a zoonotic potential. Surprisingly, mixtures of genotypes in individual isolates were repeatedly observed. Possible explanations are the uptake of genetically different Giardia cysts by a host, or subsequent infection of an already infected host, likely without overt symptoms, with a different Giardia species, which may cause disease. Other explanations for mixed genotypes, particularly for assemblage B, are substantial allelic sequence heterogeneity and/or genetic recombination. Although the zoonotic potential of G. duodenalis is evident, evidence on the contribution and frequency is (still) lacking. This newly developed molecular database has the potential to tackle intricate epidemiological questions concerning protozoan diseases. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_ecae2c81fa082b4e3c2f654c8bab7029 |
| institution | Instituto de Salud Carlos III (ISCIII) |
| instname_str | Instituto de Salud Carlos III (ISCIII) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repisalud.isciii.es:20.500.12105/16292 |
| publishDate | 2009 |
| publishDateSort | 2009 |
| reponame_str | Repisalud |
| spelling | Identification of zoonotic genotypes of Giardia duodenalis Sprong, Hein Cacciò, Simone M van der Giessen, Joke W B ZOOPNET network and partners Rubio Muñoz, Jose Miguel Fuentes Corripio, Isabel Animals Cats Cattle DNA, Protozoan Dogs Genetic Variation Genotype Giardia lamblia Giardiasis Goats Humans Molecular Sequence Data Phylogeny Sheep Swine Zoonoses Giardia duodenalis, originally regarded as a commensal organism, is the etiologic agent of giardiasis, a gastrointestinal disease of humans and animals. Giardiasis causes major public and veterinary health concerns worldwide. Transmission is either direct, through the faecal-oral route, or indirect, through ingestion of contaminated water or food. Genetic characterization of G. duodenalis isolates has revealed the existence of seven groups (assemblages A to G) which differ in their host distribution. Assemblages A and B are found in humans and in many other mammals, but the role of animals in the epidemiology of human infection is still unclear, despite the fact that the zoonotic potential of Giardia was recognised by the WHO some 30 years ago. Here, we performed an extensive genetic characterization of 978 human and 1440 animal isolates, which together comprise 3886 sequences from 4 genetic loci. The data were assembled into a molecular epidemiological database developed by a European network of public and veterinary health Institutions. Genotyping was performed at different levels of resolution (single and multiple loci on the same dataset). The zoonotic potential of both assemblages A and B is evident when studied at the level of assemblages, sub-assemblages, and even at each single locus. However, when genotypes are defined using a multi-locus sequence typing scheme, only 2 multi-locus genotypes (MLG) of assemblage A and none of assemblage B appear to have a zoonotic potential. Surprisingly, mixtures of genotypes in individual isolates were repeatedly observed. Possible explanations are the uptake of genetically different Giardia cysts by a host, or subsequent infection of an already infected host, likely without overt symptoms, with a different Giardia species, which may cause disease. Other explanations for mixed genotypes, particularly for assemblage B, are substantial allelic sequence heterogeneity and/or genetic recombination. Although the zoonotic potential of G. duodenalis is evident, evidence on the contribution and frequency is (still) lacking. This newly developed molecular database has the potential to tackle intricate epidemiological questions concerning protozoan diseases. http://hdl.handle.net/20.500.12105/16292 |
| spellingShingle | Identification of zoonotic genotypes of Giardia duodenalis Sprong, Hein Animals Cats Cattle DNA, Protozoan Dogs Genetic Variation Genotype Giardia lamblia Giardiasis Goats Humans Molecular Sequence Data Phylogeny Sheep Swine Zoonoses |
| title | Identification of zoonotic genotypes of Giardia duodenalis |
| title_full | Identification of zoonotic genotypes of Giardia duodenalis |
| title_fullStr | Identification of zoonotic genotypes of Giardia duodenalis |
| title_full_unstemmed | Identification of zoonotic genotypes of Giardia duodenalis |
| title_short | Identification of zoonotic genotypes of Giardia duodenalis |
| title_sort | Identification of zoonotic genotypes of Giardia duodenalis |
| topic | Animals Cats Cattle DNA, Protozoan Dogs Genetic Variation Genotype Giardia lamblia Giardiasis Goats Humans Molecular Sequence Data Phylogeny Sheep Swine Zoonoses |
| topic_facet | Animals Cats Cattle DNA, Protozoan Dogs Genetic Variation Genotype Giardia lamblia Giardiasis Goats Humans Molecular Sequence Data Phylogeny Sheep Swine Zoonoses |
| url | http://hdl.handle.net/20.500.12105/16292 |