In vitro fabrication of microscale secretory granules
Advanced medical treatments involving drug delivery require fully biocompatible materials with the ability to release functional drugs in a time-prolonged way. Ideally, the delivered molecules should be self-contained as chemically homogenous entities to prevent the use of potentially toxic scaffold...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2021 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:264010 |
| Online Access: | https://ddd.uab.cat/record/264010 https://dx.doi.org/urn:doi:10.1002/adfm.202100914 |
| Access Level: | Open access |
| Keyword: | Protein materials Microparticles Divalent cations Secretory granules Drug release |
| _version_ | 1878741725740007424 |
|---|---|
| author | López-Laguna, Hèctor|||0000-0001-5249-8304 |
| author2 | Parladé Molist, Eloi|||0000-0001-5750-550X Álamo, Patricia|||0000-0003-0510-5701 Sánchez, Julieta M.|||0000-0001-6676-5776 Voltà-Durán, Eric|||0000-0003-0017-8274 Serna, Naroa|||0000-0001-5682-8198 Sánchez-García, Laura|||0000-0002-8420-1701 Cano-Garrido, Olivia|||0000-0002-5504-2131 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 Villaverde, Antonio|||0000-0002-2615-4521 Mangues, Ramon|||0000-0003-2661-9525 Unzueta Elorza, Ugutz|||0000-0001-5119-2266 Vázquez, Esther|||0000-0003-1052-0424 |
| author2_role | author author author author author author author author author author author author |
| author_browse | Cano-Garrido, Olivia|||0000-0002-5504-2131 López-Laguna, Hèctor|||0000-0001-5249-8304 Mangues, Ramon|||0000-0003-2661-9525 Parladé Molist, Eloi|||0000-0001-5750-550X Serna, Naroa|||0000-0001-5682-8198 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 Sánchez, Julieta M.|||0000-0001-6676-5776 Sánchez-García, Laura|||0000-0002-8420-1701 Unzueta Elorza, Ugutz|||0000-0001-5119-2266 Villaverde, Antonio|||0000-0002-2615-4521 Voltà-Durán, Eric|||0000-0003-0017-8274 Vázquez, Esther|||0000-0003-1052-0424 Álamo, Patricia|||0000-0003-0510-5701 |
| author_facet | López-Laguna, Hèctor|||0000-0001-5249-8304 Parladé Molist, Eloi|||0000-0001-5750-550X Álamo, Patricia|||0000-0003-0510-5701 Sánchez, Julieta M.|||0000-0001-6676-5776 Voltà-Durán, Eric|||0000-0003-0017-8274 Serna, Naroa|||0000-0001-5682-8198 Sánchez-García, Laura|||0000-0002-8420-1701 Cano-Garrido, Olivia|||0000-0002-5504-2131 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 Villaverde, Antonio|||0000-0002-2615-4521 Mangues, Ramon|||0000-0003-2661-9525 Unzueta Elorza, Ugutz|||0000-0001-5119-2266 Vázquez, Esther|||0000-0003-1052-0424 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Advanced medical treatments involving drug delivery require fully biocompatible materials with the ability to release functional drugs in a time-prolonged way. Ideally, the delivered molecules should be self-contained as chemically homogenous entities to prevent the use of potentially toxic scaffolds or hold matrices. In nature, peptidic hormones are self-stored in protein-only secretory granules formed by the reversible coordination of Zn and histidine residues. Inspired by this concept, an in vitro transversal procedure is developed, analyzed, and comparatively applied for the fabrication of protein-only secretory granules at the microscale. These materials can be produced from any polyhistidine-tagged protein using physiological concentrations of Zn as a potent and versatile glue-like agent. The screening of granules formed by 12 engineered and nonengineered proteins at different Zn concentrations revealed optimal fabrication conditions and the consequent release profiles. Moreover, the functional and structural properties of the delivered protein are fully validated using a drug-targeting protein platform in a mouse model of human colorectal cancer. In summary, short histidine tags allow the packaging of structurally and functionally dissimilar polypeptides, which supports the proposed fabrication method as a powerful protocol extensible to diverse clinical scenarios in which slow protein drug delivery is required. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_ea4feb9dbb01de1d1336e5e2a8b05f2e |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:264010 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | In vitro fabrication of microscale secretory granules López-Laguna, Hèctor|||0000-0001-5249-8304 Parladé Molist, Eloi|||0000-0001-5750-550X Álamo, Patricia|||0000-0003-0510-5701 Sánchez, Julieta M.|||0000-0001-6676-5776 Voltà-Durán, Eric|||0000-0003-0017-8274 Serna, Naroa|||0000-0001-5682-8198 Sánchez-García, Laura|||0000-0002-8420-1701 Cano-Garrido, Olivia|||0000-0002-5504-2131 Sánchez Chardi, Alejandro|||0000-0002-8789-1883 Villaverde, Antonio|||0000-0002-2615-4521 Mangues, Ramon|||0000-0003-2661-9525 Unzueta Elorza, Ugutz|||0000-0001-5119-2266 Vázquez, Esther|||0000-0003-1052-0424 Protein materials Microparticles Divalent cations Secretory granules Drug release Advanced medical treatments involving drug delivery require fully biocompatible materials with the ability to release functional drugs in a time-prolonged way. Ideally, the delivered molecules should be self-contained as chemically homogenous entities to prevent the use of potentially toxic scaffolds or hold matrices. In nature, peptidic hormones are self-stored in protein-only secretory granules formed by the reversible coordination of Zn and histidine residues. Inspired by this concept, an in vitro transversal procedure is developed, analyzed, and comparatively applied for the fabrication of protein-only secretory granules at the microscale. These materials can be produced from any polyhistidine-tagged protein using physiological concentrations of Zn as a potent and versatile glue-like agent. The screening of granules formed by 12 engineered and nonengineered proteins at different Zn concentrations revealed optimal fabrication conditions and the consequent release profiles. Moreover, the functional and structural properties of the delivered protein are fully validated using a drug-targeting protein platform in a mouse model of human colorectal cancer. In summary, short histidine tags allow the packaging of structurally and functionally dissimilar polypeptides, which supports the proposed fabrication method as a powerful protocol extensible to diverse clinical scenarios in which slow protein drug delivery is required. https://ddd.uab.cat/record/264010 https://dx.doi.org/urn:doi:10.1002/adfm.202100914 |
| spellingShingle | In vitro fabrication of microscale secretory granules López-Laguna, Hèctor|||0000-0001-5249-8304 Protein materials Microparticles Divalent cations Secretory granules Drug release |
| title | In vitro fabrication of microscale secretory granules |
| title_full | In vitro fabrication of microscale secretory granules |
| title_fullStr | In vitro fabrication of microscale secretory granules |
| title_full_unstemmed | In vitro fabrication of microscale secretory granules |
| title_short | In vitro fabrication of microscale secretory granules |
| title_sort | In vitro fabrication of microscale secretory granules |
| topic | Protein materials Microparticles Divalent cations Secretory granules Drug release |
| topic_facet | Protein materials Microparticles Divalent cations Secretory granules Drug release |
| url | https://ddd.uab.cat/record/264010 https://dx.doi.org/urn:doi:10.1002/adfm.202100914 |