Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods
In this study, we validated various methods of pre-treatment of poly(ethylene terephthalate) (PET) by the engineered yeast Yarrowia lipolytica. This research compares both the effect of the type of plastic used, the processing method and enzymes with different mechanisms of action (PETase and cutina...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2024 |
| Country: | España |
| Institution: | Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) |
| Repository: | Docu-menta. Repositorio Institucional del CIEMAT |
| Language: | English |
| OAI Identifier: | oai:dnet:documenta___::c569f2890dd9437fa8480479e825d64b |
| Online Access: | https://hdl.handle.net/20.500.14855/4801 |
| Access Level: | Open access |
| _version_ | 1878741700373905409 |
|---|---|
| author | Kosiorowska, Katarzyna E. |
| author2 | Moreno, Antonio D. Iglesias, Raquel Biniarz, Piotr Mirończuk, Aleksandra M. |
| author2_role | author author author author |
| author_browse | Biniarz, Piotr Iglesias, Raquel Kosiorowska, Katarzyna E. Mirończuk, Aleksandra M. Moreno, Antonio D. |
| author_facet | Kosiorowska, Katarzyna E. Moreno, Antonio D. Iglesias, Raquel Biniarz, Piotr Mirończuk, Aleksandra M. |
| author_role | author |
| collection | Docu-menta. Repositorio Institucional del CIEMAT |
| description | In this study, we validated various methods of pre-treatment of poly(ethylene terephthalate) (PET) by the engineered yeast Yarrowia lipolytica. This research compares both the effect of the type of plastic used, the processing method and enzymes with different mechanisms of action (PETase and cutinase). The investigation demonstrated that the degradation efficiency varies depending on the type of plastic used, the processing methods and the applied enzyme. Moreover, it indicated that during prolonged yeast culture under the applied conditions, enzyme activity is not impaired. Among all the methods tested, the artificial aging process had the greatest impact on the degradation level by PETase, where the amount of TPA released from commercial PET film was the highest, and yielded over 2 gL-1. The maximum yield of TPA (0.59 gL-1), for the Y. lipolytica strain overexpressing cutinase, was observed during the process with recycled PET bottles shredded into 1 mm fragments. The maximum recorded weight loss of plastic film is over 70% for commercial PET film subjected to artificial ageing process. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e9daf8a549e5d3579d3a9f261cc55696 |
| institution | Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) |
| instname_str | Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:dnet:documenta___::c569f2890dd9437fa8480479e825d64b |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | Docu-menta. Repositorio Institucional del CIEMAT |
| spelling | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods Kosiorowska, Katarzyna E. Moreno, Antonio D. Iglesias, Raquel Biniarz, Piotr Mirończuk, Aleksandra M. In this study, we validated various methods of pre-treatment of poly(ethylene terephthalate) (PET) by the engineered yeast Yarrowia lipolytica. This research compares both the effect of the type of plastic used, the processing method and enzymes with different mechanisms of action (PETase and cutinase). The investigation demonstrated that the degradation efficiency varies depending on the type of plastic used, the processing methods and the applied enzyme. Moreover, it indicated that during prolonged yeast culture under the applied conditions, enzyme activity is not impaired. Among all the methods tested, the artificial aging process had the greatest impact on the degradation level by PETase, where the amount of TPA released from commercial PET film was the highest, and yielded over 2 gL-1. The maximum yield of TPA (0.59 gL-1), for the Y. lipolytica strain overexpressing cutinase, was observed during the process with recycled PET bottles shredded into 1 mm fragments. The maximum recorded weight loss of plastic film is over 70% for commercial PET film subjected to artificial ageing process. Elsevier https://hdl.handle.net/20.500.14855/4801 |
| spellingShingle | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods Kosiorowska, Katarzyna E. |
| title | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods |
| title_full | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods |
| title_fullStr | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods |
| title_full_unstemmed | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods |
| title_short | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods |
| title_sort | Streamlining biological recycling of poly(ethylene terephthalate) via pre-treatment methods |
| url | https://hdl.handle.net/20.500.14855/4801 |