X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk
X-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here, we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells, XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF...
| Authors: | ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2013 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:115507 |
| Online Access: | https://ddd.uab.cat/record/115507 https://dx.doi.org/urn:doi:10.1038/srep02397 |
| Access Level: | Open access |
| Keyword: | Neurociència Factors neurotròfics Neurociència cel·lular Creixement cel·lular Neuroquímica Neurotrophic factors Cellular neuroscience Cell growth Neurochemistry |
| _version_ | 1878741678042382336 |
|---|---|
| author | Fadó, Rut|||0000-0002-3293-2342 |
| author2 | Moubarak, Rana S.|||0000-0003-2096-1258 Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682 Barneda Zahonero, Bruna|||0000-0002-6557-5222 Valero Gomez-Lobo, Jorge Saura Antolín, Carlos|||0000-0003-3692-5657 Morán, Julio Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786 Rodríguez Álvarez, José|||0000-0001-8582-8082 |
| author2_role | author author author author author author author author |
| author_browse | Barneda Zahonero, Bruna|||0000-0002-6557-5222 Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786 Fadó, Rut|||0000-0002-3293-2342 Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682 Morán, Julio Moubarak, Rana S.|||0000-0003-2096-1258 Rodríguez Álvarez, José|||0000-0001-8582-8082 Saura Antolín, Carlos|||0000-0003-3692-5657 Valero Gomez-Lobo, Jorge |
| author_facet | Fadó, Rut|||0000-0002-3293-2342 Moubarak, Rana S.|||0000-0003-2096-1258 Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682 Barneda Zahonero, Bruna|||0000-0002-6557-5222 Valero Gomez-Lobo, Jorge Saura Antolín, Carlos|||0000-0003-3692-5657 Morán, Julio Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786 Rodríguez Álvarez, José|||0000-0001-8582-8082 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | X-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here, we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells, XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF treatment induces a reduction of endogenous XIAP levels concomitant with the induction of neuronal differentiation. Accordingly, downregulation of endogenous XIAP protein levels strongly increases neurite outgrowth in PC12 cells as well as axonal and dendritic length in primary cortical neurons. The effects of XIAP are mediated by the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinases (ERKs) pathway since blocking this pathway completely prevents the neuritogenesis mediated by XIAP downregulation. In addition, we found that XIAP binds to cRaf and Trk receptors. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e96f37f8bfecc605b0f010400a2cb863 |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:115507 |
| publishDate | 2013 |
| publishDateSort | 2013 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk Fadó, Rut|||0000-0002-3293-2342 Moubarak, Rana S.|||0000-0003-2096-1258 Miñano Molina, Alfredo Jesús|||0000-0002-7761-5682 Barneda Zahonero, Bruna|||0000-0002-6557-5222 Valero Gomez-Lobo, Jorge Saura Antolín, Carlos|||0000-0003-3692-5657 Morán, Julio Comella i Carnicé, Joan Xavier|||0000-0002-6218-0786 Rodríguez Álvarez, José|||0000-0001-8582-8082 Neurociència Factors neurotròfics Neurociència cel·lular Creixement cel·lular Neuroquímica Neurotrophic factors Cellular neuroscience Cell growth Neurochemistry X-linked Inhibitor of apoptosis protein (XIAP) has been classically identified as a cell death regulator. Here, we demonstrate a novel function of XIAP as a regulator of neurite outgrowth in neuronal cells. In PC12 cells, XIAP overexpression prevents NGF-induced neuronal differentiation, whereas NGF treatment induces a reduction of endogenous XIAP levels concomitant with the induction of neuronal differentiation. Accordingly, downregulation of endogenous XIAP protein levels strongly increases neurite outgrowth in PC12 cells as well as axonal and dendritic length in primary cortical neurons. The effects of XIAP are mediated by the mitogen-activated protein kinase (MEK)/extracellular signal-regulated kinases (ERKs) pathway since blocking this pathway completely prevents the neuritogenesis mediated by XIAP downregulation. In addition, we found that XIAP binds to cRaf and Trk receptors. Our results demonstrate that XIAP plays a new role as a negative regulator of neurotrophin-induced neurite outgrowth and neuronal differentiation in developing neurons. https://ddd.uab.cat/record/115507 https://dx.doi.org/urn:doi:10.1038/srep02397 |
| spellingShingle | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk Fadó, Rut|||0000-0002-3293-2342 Neurociència Factors neurotròfics Neurociència cel·lular Creixement cel·lular Neuroquímica Neurotrophic factors Cellular neuroscience Cell growth Neurochemistry |
| title | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk |
| title_full | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk |
| title_fullStr | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk |
| title_full_unstemmed | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk |
| title_short | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk |
| title_sort | X-linked Inhibitor of Apoptosis Protein negatively regulates neuronal differentiation through interaction with cRAF and Trk |
| topic | Neurociència Factors neurotròfics Neurociència cel·lular Creixement cel·lular Neuroquímica Neurotrophic factors Cellular neuroscience Cell growth Neurochemistry |
| topic_facet | Neurociència Factors neurotròfics Neurociència cel·lular Creixement cel·lular Neuroquímica Neurotrophic factors Cellular neuroscience Cell growth Neurochemistry |
| url | https://ddd.uab.cat/record/115507 https://dx.doi.org/urn:doi:10.1038/srep02397 |