Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2
Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Universidad Complutense de Madrid (UCM) |
| Repository: | Docta Complutense |
| Language: | English |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/104218 |
| Online Access: | https://hdl.handle.net/20.500.14352/104218 |
| Access Level: | Open access |
| Keyword: | 577.1/2 Adult neurogenesis Neural specification Neural stem cell Neurogenin 2 Adult subventricular zone Olfactory bulb Fate reprogramming Proneural gene Bioquímica (Biología) Biología molecular (Biología) 2403 Bioquímica |
| _version_ | 1878741653303328769 |
|---|---|
| author | Péron, Sophie |
| author2 | Miyakoshi, Leo M. Brill, Monika S. Manzano Franco, Diana Serrano López, Julia Fan, Wenqiang Marichal, Nicolás Ghanem, Alexander Conzelmann, Karl-Klaus Karow, Marisa Ortega De La O, Felipe Gascón Jiménez, Sergio Berninger, Benedikt |
| author2_role | author author author author author author author author author author author author |
| author_browse | Berninger, Benedikt Brill, Monika S. Conzelmann, Karl-Klaus Fan, Wenqiang Gascón Jiménez, Sergio Ghanem, Alexander Karow, Marisa Manzano Franco, Diana Marichal, Nicolás Miyakoshi, Leo M. Ortega De La O, Felipe Péron, Sophie Serrano López, Julia |
| author_facet | Péron, Sophie Miyakoshi, Leo M. Brill, Monika S. Manzano Franco, Diana Serrano López, Julia Fan, Wenqiang Marichal, Nicolás Ghanem, Alexander Conzelmann, Karl-Klaus Karow, Marisa Ortega De La O, Felipe Gascón Jiménez, Sergio Berninger, Benedikt |
| author_role | author |
| collection | Docta Complutense |
| description | Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a glutamatergic fate. Retrovirus-mediated expression of Neurog2 induced the glutamatergic lineage markers TBR2 and TBR1 in cultured SEZ progenitors, which differentiated into functional glutamatergic neurons. Likewise, Neurog2-transduced SEZ progenitors acquired glutamatergic neuron hallmarks in vivo. Intriguingly, they failed to migrate toward the olfactory bulb and instead differentiated within the SEZ or the adjacent striatum, where they received connections from local neurons, as indicated by rabies virus-mediated monosynaptic tracing. In contrast, lentivirus-mediated expression of Neurog2 failed to reprogram early SEZ neurons, which maintained GABAergic identity and migrated to the olfactory bulb. Our data show that NEUROG2 can program SEZ progenitors toward a glutamatergic identity but fails to reprogram their neuronal progeny. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e905f7608b5439ccf207c0d3bb65f90f |
| institution | Universidad Complutense de Madrid (UCM) |
| instname_str | Universidad Complutense de Madrid (UCM) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:docta.ucm.es:20.500.14352/104218 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | Cell Press |
| publisherStr | Cell Press |
| reponame_str | Docta Complutense |
| spelling | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 Péron, Sophie Miyakoshi, Leo M. Brill, Monika S. Manzano Franco, Diana Serrano López, Julia Fan, Wenqiang Marichal, Nicolás Ghanem, Alexander Conzelmann, Karl-Klaus Karow, Marisa Ortega De La O, Felipe Gascón Jiménez, Sergio Berninger, Benedikt 577.1/2 Adult neurogenesis Neural specification Neural stem cell Neurogenin 2 Adult subventricular zone Olfactory bulb Fate reprogramming Proneural gene Bioquímica (Biología) Biología molecular (Biología) 2403 Bioquímica Although adult subependymal zone (SEZ) neural stem cells mostly generate GABAergic interneurons, a small progenitor population expresses the proneural gene Neurog2 and produces glutamatergic neurons. Here, we determined whether Neurog2 could respecify SEZ neural stem cells and their progeny toward a glutamatergic fate. Retrovirus-mediated expression of Neurog2 induced the glutamatergic lineage markers TBR2 and TBR1 in cultured SEZ progenitors, which differentiated into functional glutamatergic neurons. Likewise, Neurog2-transduced SEZ progenitors acquired glutamatergic neuron hallmarks in vivo. Intriguingly, they failed to migrate toward the olfactory bulb and instead differentiated within the SEZ or the adjacent striatum, where they received connections from local neurons, as indicated by rabies virus-mediated monosynaptic tracing. In contrast, lentivirus-mediated expression of Neurog2 failed to reprogram early SEZ neurons, which maintained GABAergic identity and migrated to the olfactory bulb. Our data show that NEUROG2 can program SEZ progenitors toward a glutamatergic identity but fails to reprogram their neuronal progeny. Cell Press https://hdl.handle.net/20.500.14352/104218 |
| spellingShingle | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 Péron, Sophie 577.1/2 Adult neurogenesis Neural specification Neural stem cell Neurogenin 2 Adult subventricular zone Olfactory bulb Fate reprogramming Proneural gene Bioquímica (Biología) Biología molecular (Biología) 2403 Bioquímica |
| title | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 |
| title_full | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 |
| title_fullStr | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 |
| title_full_unstemmed | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 |
| title_short | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 |
| title_sort | Programming of neural progenitors of the adult subependymal zone towards a glutamatergic neuron lineage by neurogenin 2 |
| topic | 577.1/2 Adult neurogenesis Neural specification Neural stem cell Neurogenin 2 Adult subventricular zone Olfactory bulb Fate reprogramming Proneural gene Bioquímica (Biología) Biología molecular (Biología) 2403 Bioquímica |
| topic_facet | 577.1/2 Adult neurogenesis Neural specification Neural stem cell Neurogenin 2 Adult subventricular zone Olfactory bulb Fate reprogramming Proneural gene Bioquímica (Biología) Biología molecular (Biología) 2403 Bioquímica |
| url | https://hdl.handle.net/20.500.14352/104218 |