Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.)
https://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591
| Autores: | ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de La Laguna (ULL) |
| Repositorio: | RIULL. Repositorio Institucional de la Universidad de La Laguna |
| OAI Identifier: | oai:riull.ull.es:915/41771 |
| Acceso en línea: | http://riull.ull.es/xmlui/handle/915/41771 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | cultivars iPBS LTR-retrotransposon microsatellite Musa SSR |
| _version_ | 1878741524481572864 |
|---|---|
| author | González Carracedo, Mario Andrés |
| author2 | Tejera Pérez, Hugo Hernández Ferrer, Mariano Jiménez Arias, David Pérez Pérez, José Antonio |
| author2_role | author author author author |
| author_browse | González Carracedo, Mario Andrés Hernández Ferrer, Mariano Jiménez Arias, David Pérez Pérez, José Antonio Tejera Pérez, Hugo |
| author_facet | González Carracedo, Mario Andrés Tejera Pérez, Hugo Hernández Ferrer, Mariano Jiménez Arias, David Pérez Pérez, José Antonio |
| author_role | author |
| collection | RIULL. Repositorio Institucional de la Universidad de La Laguna |
| description | https://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e6bab948600c20d933253eec79d65e8a |
| institution | Universidad de La Laguna (ULL) |
| instname_str | Universidad de La Laguna (ULL) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:riull.ull.es:915/41771 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| reponame_str | RIULL. Repositorio Institucional de la Universidad de La Laguna |
| spelling | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) González Carracedo, Mario Andrés Tejera Pérez, Hugo Hernández Ferrer, Mariano Jiménez Arias, David Pérez Pérez, José Antonio cultivars iPBS LTR-retrotransposon microsatellite Musa SSR https://portalciencia.ull.es/documentos/6132c4bf33c0636613bd9591 Banana cultivars of agronomic interest have been genetically characterized using two different molecular markers. On the one hand, a panel of 14 trinucleotide single sequence repeats (SSRs or microsatellites) was optimized for homogeneous PCR conditions. It was tested with 50 individuals from seven cultivars, yielding 76 alleles and 5.4 ± 1.8 alleles per locus, while the presence of cultivar-exclusive alleles allowed the discrimination of all cultivars. On the other hand, a retrotransposon-based marker system named inter-primer binding site (iPBS) was implemented for the first time in the Musa genus. A total of 120 bands were detected in eight different Musa cultivars, from which 65.8% were polymorphic and 23.3% were cultivar exclusive. Both techniques allowed a cut-off identification of all cultivars studied, but overall, iPBS analysis was a more straightforward and economical choice. Despite the fact that we were unable to distinguish local banana varieties belonging to the same cultivar, new cultivar-specific molecular markers have been developed for the Musa genus, which could be used to guide new breeding programmes and maintain high quality of Plátano de Canarias. http://riull.ull.es/xmlui/handle/915/41771 |
| spellingShingle | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) González Carracedo, Mario Andrés cultivars iPBS LTR-retrotransposon microsatellite Musa SSR |
| title | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) |
| title_full | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) |
| title_fullStr | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) |
| title_full_unstemmed | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) |
| title_short | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) |
| title_sort | Comparative assessment of microsatellite and retrotransposon-based markers for genetic characterization of commercial banana cultivars (Musa spp.) |
| topic | cultivars iPBS LTR-retrotransposon microsatellite Musa SSR |
| topic_facet | cultivars iPBS LTR-retrotransposon microsatellite Musa SSR |
| url | http://riull.ull.es/xmlui/handle/915/41771 |