Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identi...
| Authors: | ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2012 |
| Country: | España |
| Institution: | Universidad de Sevilla (US) |
| Repository: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/36412 |
| Online Access: | http://hdl.handle.net/11441/36412 https://doi.org/10.1128/AEM.01552-12 |
| Access Level: | Open access |
| Keyword: | Agar culture Conidiation Fusarium Fusarium oxysporums Fusarium species High sensitivity MRNA level Nitrogen availability Plant tissues Polyketide synthase genes Polyketide synthases Polyketides Sequence comparisons Wild types |
| _version_ | 1878741373071392768 |
|---|---|
| author | Ávalos Cordero, Francisco Javier |
| author2 | Limón Mirón, María del Carmen Díaz Sánchez, Violeta |
| author2_role | author author |
| author_browse | Díaz Sánchez, Violeta Limón Mirón, María del Carmen Ávalos Cordero, Francisco Javier |
| author_facet | Ávalos Cordero, Francisco Javier Limón Mirón, María del Carmen Díaz Sánchez, Violeta |
| author_role | author |
| collection | idUS. Depósito de Investigación de la Universidad de Sevilla |
| description | Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen avail- ability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduc- tion of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cel- lophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum. The fusA mutants, however, are not affected in their capacities to grow on plant tissues. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e400a01c656f8ecb3ecae68fc74424f6 |
| institution | Universidad de Sevilla (US) |
| instname_str | Universidad de Sevilla (US) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:idus.us.es:11441/36412 |
| publishDate | 2012 |
| publishDateSort | 2012 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| reponame_str | idUS. Depósito de Investigación de la Universidad de Sevilla |
| spelling | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi Ávalos Cordero, Francisco Javier Limón Mirón, María del Carmen Díaz Sánchez, Violeta Agar culture Conidiation Fusarium Fusarium oxysporums Fusarium species High sensitivity MRNA level Nitrogen availability Plant tissues Polyketide synthase genes Polyketide synthases Polyketides Sequence comparisons Wild types Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen avail- ability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduc- tion of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cel- lophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum. The fusA mutants, however, are not affected in their capacities to grow on plant tissues. American Society for Microbiology http://hdl.handle.net/11441/36412 https://doi.org/10.1128/AEM.01552-12 |
| spellingShingle | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi Ávalos Cordero, Francisco Javier Agar culture Conidiation Fusarium Fusarium oxysporums Fusarium species High sensitivity MRNA level Nitrogen availability Plant tissues Polyketide synthase genes Polyketide synthases Polyketides Sequence comparisons Wild types |
| status_str | publishedVersion |
| title | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi |
| title_full | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi |
| title_fullStr | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi |
| title_full_unstemmed | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi |
| title_short | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi |
| title_sort | Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi |
| topic | Agar culture Conidiation Fusarium Fusarium oxysporums Fusarium species High sensitivity MRNA level Nitrogen availability Plant tissues Polyketide synthase genes Polyketide synthases Polyketides Sequence comparisons Wild types |
| topic_facet | Agar culture Conidiation Fusarium Fusarium oxysporums Fusarium species High sensitivity MRNA level Nitrogen availability Plant tissues Polyketide synthase genes Polyketide synthases Polyketides Sequence comparisons Wild types |
| url | http://hdl.handle.net/11441/36412 https://doi.org/10.1128/AEM.01552-12 |