Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
Oxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential fo...
| Autores: | ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/110235 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/110235 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | EA.hy926 Free fatty acids Reactive oxygen species Cardiovascular disease Farmacología (Farmacia) 3209 Farmacología |
| _version_ | 1878741297016078336 |
|---|---|
| author | Palomino Ruiz-Poveda, Olga María |
| author2 | Giordiani, Veronica Chowen, Julie Fernández Alfonso, María Soledad Goya, Luis |
| author2_role | author author author author |
| author_browse | Chowen, Julie Fernández Alfonso, María Soledad Giordiani, Veronica Goya, Luis Palomino Ruiz-Poveda, Olga María |
| author_facet | Palomino Ruiz-Poveda, Olga María Giordiani, Veronica Chowen, Julie Fernández Alfonso, María Soledad Goya, Luis |
| author_role | author |
| collection | Docta Complutense |
| description | Oxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential for cell and tissue function and olive oil free fatty acids have proved to exhibit beneficial effects on risk factors for cardiovascular disease. We hypothesize that realistic concentrations within the physiological range of oleic (OA) and palmitic (PA) acids could be beneficial in the prevention of oxidative stress in vascular endothelium. Hence, pre-treatment and co-treatment with realistic physiological doses of palmitic and oleic acids were tested on cultured endothelial cells submitted to a chemically induced oxidative stress to investigate their potential chemo-protective effect. Cell viability and markers of oxidative status: reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA), glutathione peroxidase (GPx) and glutathione reductase (GR) were evaluated. As a conclusion, the increased ROS generation induced by stress was significantly prevented by a pre- and co-treatment with PA or OA. Moreover, pre- and co-treatment of cells with FFAs recovered the stress-induced MDA concentration to control values and significantly recovered depleted GSH and normalized GPx and GR activities. Finally, pre- and co-treatment of cells with physiological concentrations of PA or OA in the low micromolar range conferred a substantial protection of cell viability against an oxidative insult. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e2b560e2eff25e8f0994bb2a9bc3442b |
| institution | Universidad Complutense de Madrid (UCM) |
| instname_str | Universidad Complutense de Madrid (UCM) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:docta.ucm.es:20.500.14352/110235 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Docta Complutense |
| spelling | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress Palomino Ruiz-Poveda, Olga María Giordiani, Veronica Chowen, Julie Fernández Alfonso, María Soledad Goya, Luis EA.hy926 Free fatty acids Reactive oxygen species Cardiovascular disease Farmacología (Farmacia) 3209 Farmacología Oxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential for cell and tissue function and olive oil free fatty acids have proved to exhibit beneficial effects on risk factors for cardiovascular disease. We hypothesize that realistic concentrations within the physiological range of oleic (OA) and palmitic (PA) acids could be beneficial in the prevention of oxidative stress in vascular endothelium. Hence, pre-treatment and co-treatment with realistic physiological doses of palmitic and oleic acids were tested on cultured endothelial cells submitted to a chemically induced oxidative stress to investigate their potential chemo-protective effect. Cell viability and markers of oxidative status: reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA), glutathione peroxidase (GPx) and glutathione reductase (GR) were evaluated. As a conclusion, the increased ROS generation induced by stress was significantly prevented by a pre- and co-treatment with PA or OA. Moreover, pre- and co-treatment of cells with FFAs recovered the stress-induced MDA concentration to control values and significantly recovered depleted GSH and normalized GPx and GR activities. Finally, pre- and co-treatment of cells with physiological concentrations of PA or OA in the low micromolar range conferred a substantial protection of cell viability against an oxidative insult. MDPI https://hdl.handle.net/20.500.14352/110235 |
| spellingShingle | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress Palomino Ruiz-Poveda, Olga María EA.hy926 Free fatty acids Reactive oxygen species Cardiovascular disease Farmacología (Farmacia) 3209 Farmacología |
| title | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress |
| title_full | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress |
| title_fullStr | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress |
| title_full_unstemmed | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress |
| title_short | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress |
| title_sort | Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress |
| topic | EA.hy926 Free fatty acids Reactive oxygen species Cardiovascular disease Farmacología (Farmacia) 3209 Farmacología |
| topic_facet | EA.hy926 Free fatty acids Reactive oxygen species Cardiovascular disease Farmacología (Farmacia) 3209 Farmacología |
| url | https://hdl.handle.net/20.500.14352/110235 |