Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica)
Defining hidden genetic diversity within species is of great significance when attempting to maintain the evolutionary potential of natural populations and conduct appropriate management. Our hypothesis is that isolated (and eventually small) wild animal populations hide unexpected genetic diversity...
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| Tipo de documento: | Artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:ddd.uab.cat:190814 |
| Acceso en línea: | https://ddd.uab.cat/record/190814 https://dx.doi.org/urn:doi:10.1186/s12863-018-0616-9 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Capra pyrenaica hispanica Capra pyrenaica victoriae Capra aegagrus hircus Major histocompatibility complex (MHC) MHC DRB1 OLADRB1 Linkage disequilibrium DRB-STR method Sierras de Cazorla Segura and las Villas Natural Park Spain |
| _version_ | 1878741176657379328 |
|---|---|
| author | Angelone, Samer|||0000-0003-0868-7276 |
| author2 | Jowers, Michael J. Molinar Min, Anna Rita Fandos, Paulino|||0000-0002-9607-8931 Prieto, Paloma Pasquetti, Mario Cano-Manuel, Francisco Javier Mentaberre García, Gregorio|||0000-0001-9542-7514 López Olvera, Jorge R.|||0000-0002-2999-3451 Ráez-Bravo, Arián|||0000-0002-3190-1659 Espinosa, José|||0000-0002-9036-1402 Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Soriguer, Ramón C.|||0000-0002-9165-7766 Rossi, Luca|||0000-0001-6829-5229 Granados Torres, José Enrique|||0000-0002-9787-9896 |
| author2_role | author author author author author author author author author author author author author author |
| author_browse | Angelone, Samer|||0000-0003-0868-7276 Cano-Manuel, Francisco Javier Espinosa, José|||0000-0002-9036-1402 Fandos, Paulino|||0000-0002-9607-8931 Granados Torres, José Enrique|||0000-0002-9787-9896 Jowers, Michael J. López Olvera, Jorge R.|||0000-0002-2999-3451 Mentaberre García, Gregorio|||0000-0001-9542-7514 Molinar Min, Anna Rita Pasquetti, Mario Prieto, Paloma Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Rossi, Luca|||0000-0001-6829-5229 Ráez-Bravo, Arián|||0000-0002-3190-1659 Soriguer, Ramón C.|||0000-0002-9165-7766 |
| author_facet | Angelone, Samer|||0000-0003-0868-7276 Jowers, Michael J. Molinar Min, Anna Rita Fandos, Paulino|||0000-0002-9607-8931 Prieto, Paloma Pasquetti, Mario Cano-Manuel, Francisco Javier Mentaberre García, Gregorio|||0000-0001-9542-7514 López Olvera, Jorge R.|||0000-0002-2999-3451 Ráez-Bravo, Arián|||0000-0002-3190-1659 Espinosa, José|||0000-0002-9036-1402 Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Soriguer, Ramón C.|||0000-0002-9165-7766 Rossi, Luca|||0000-0001-6829-5229 Granados Torres, José Enrique|||0000-0002-9787-9896 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Defining hidden genetic diversity within species is of great significance when attempting to maintain the evolutionary potential of natural populations and conduct appropriate management. Our hypothesis is that isolated (and eventually small) wild animal populations hide unexpected genetic diversity due to their maintenance of ancient polymorphisms or introgressions. We tested this hypothesis using the Iberian ibex (Capra pyrenaica) as an example. Previous studies based on large sample sizes taken from its principal populations have revealed that the Iberian ibex has a remarkably small MHC DRB1 diversity (only six remnant alleles) as a result of recent population bottlenecks and a marked demographic decline that has led to the extinction of two recognized subspecies. Extending on the geographic range to include non-studied isolated Iberian ibex populations, we sequenced a new MHC DRB1 in what seemed three small isolated populations in Southern Spain (n = 132). The findings indicate a higher genetic diversity than previously reported in this important gene. The newly discovered allele, MHC DRB1*7, is identical to one reported in the domestic goat C. aegagrus hircus. Whether or not this is the result of ancient polymorphisms maintained by balancing selection or, alternatively, introgressions from domestic goats through hybridization needs to be clarified in future studies. However, hybridization between Iberian ibex and domestic goats has been reported in Spain and the fact that the newly discovered allele is only present in one of the small isolated populations and not in the others suggests introgression. The new discovered allele is not expected to increase fitness in C. pyrenaica since it generates the same protein as the existing MHC DRB1*6. Analysis of a microsatellite locus (OLADRB1) near the new MHC DRB1*7 gene reveals a linkage disequilibrium between these two loci. The allele OLADRB1, 187 bp in length, was unambiguously linked to the MHC DRB1*7 allele. This enabled us to perform a DRB-STR matching method for the recently discovered MHC allele. This finding is critical for the conservation of the Iberian ibex since it directly affects the identification of the units of this species that should be managed and conserved separately (Evolutionarily Significant Units). The online version of this article (10.1186/s12863-018-0616-9) contains supplementary material, which is available to authorized users. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_e07fbb50d4e8b087a7d337a5abb9e0fb |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:190814 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) Angelone, Samer|||0000-0003-0868-7276 Jowers, Michael J. Molinar Min, Anna Rita Fandos, Paulino|||0000-0002-9607-8931 Prieto, Paloma Pasquetti, Mario Cano-Manuel, Francisco Javier Mentaberre García, Gregorio|||0000-0001-9542-7514 López Olvera, Jorge R.|||0000-0002-2999-3451 Ráez-Bravo, Arián|||0000-0002-3190-1659 Espinosa, José|||0000-0002-9036-1402 Pérez Jiménez, Jesús María|||0000-0001-9159-0365 Soriguer, Ramón C.|||0000-0002-9165-7766 Rossi, Luca|||0000-0001-6829-5229 Granados Torres, José Enrique|||0000-0002-9787-9896 Capra pyrenaica hispanica Capra pyrenaica victoriae Capra aegagrus hircus Major histocompatibility complex (MHC) MHC DRB1 OLADRB1 Linkage disequilibrium DRB-STR method Sierras de Cazorla Segura and las Villas Natural Park Spain Defining hidden genetic diversity within species is of great significance when attempting to maintain the evolutionary potential of natural populations and conduct appropriate management. Our hypothesis is that isolated (and eventually small) wild animal populations hide unexpected genetic diversity due to their maintenance of ancient polymorphisms or introgressions. We tested this hypothesis using the Iberian ibex (Capra pyrenaica) as an example. Previous studies based on large sample sizes taken from its principal populations have revealed that the Iberian ibex has a remarkably small MHC DRB1 diversity (only six remnant alleles) as a result of recent population bottlenecks and a marked demographic decline that has led to the extinction of two recognized subspecies. Extending on the geographic range to include non-studied isolated Iberian ibex populations, we sequenced a new MHC DRB1 in what seemed three small isolated populations in Southern Spain (n = 132). The findings indicate a higher genetic diversity than previously reported in this important gene. The newly discovered allele, MHC DRB1*7, is identical to one reported in the domestic goat C. aegagrus hircus. Whether or not this is the result of ancient polymorphisms maintained by balancing selection or, alternatively, introgressions from domestic goats through hybridization needs to be clarified in future studies. However, hybridization between Iberian ibex and domestic goats has been reported in Spain and the fact that the newly discovered allele is only present in one of the small isolated populations and not in the others suggests introgression. The new discovered allele is not expected to increase fitness in C. pyrenaica since it generates the same protein as the existing MHC DRB1*6. Analysis of a microsatellite locus (OLADRB1) near the new MHC DRB1*7 gene reveals a linkage disequilibrium between these two loci. The allele OLADRB1, 187 bp in length, was unambiguously linked to the MHC DRB1*7 allele. This enabled us to perform a DRB-STR matching method for the recently discovered MHC allele. This finding is critical for the conservation of the Iberian ibex since it directly affects the identification of the units of this species that should be managed and conserved separately (Evolutionarily Significant Units). The online version of this article (10.1186/s12863-018-0616-9) contains supplementary material, which is available to authorized users. https://ddd.uab.cat/record/190814 https://dx.doi.org/urn:doi:10.1186/s12863-018-0616-9 |
| spellingShingle | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) Angelone, Samer|||0000-0003-0868-7276 Capra pyrenaica hispanica Capra pyrenaica victoriae Capra aegagrus hircus Major histocompatibility complex (MHC) MHC DRB1 OLADRB1 Linkage disequilibrium DRB-STR method Sierras de Cazorla Segura and las Villas Natural Park Spain |
| title | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) |
| title_full | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) |
| title_fullStr | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) |
| title_full_unstemmed | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) |
| title_short | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) |
| title_sort | Hidden MHC genetic diversity in the Iberian ibex (Capra pyrenaica) |
| topic | Capra pyrenaica hispanica Capra pyrenaica victoriae Capra aegagrus hircus Major histocompatibility complex (MHC) MHC DRB1 OLADRB1 Linkage disequilibrium DRB-STR method Sierras de Cazorla Segura and las Villas Natural Park Spain |
| topic_facet | Capra pyrenaica hispanica Capra pyrenaica victoriae Capra aegagrus hircus Major histocompatibility complex (MHC) MHC DRB1 OLADRB1 Linkage disequilibrium DRB-STR method Sierras de Cazorla Segura and las Villas Natural Park Spain |
| url | https://ddd.uab.cat/record/190814 https://dx.doi.org/urn:doi:10.1186/s12863-018-0616-9 |