Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings
Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10459.1/69758 |
| Online Access: | https://doi.org/10.3390/f11080792 http://hdl.handle.net/10459.1/69758 |
| Access Level: | Open access |
| Keyword: | Black truffle Truffle colonization Edible fungi cultivation Truffle orchard Truffle trees Hypogeous ectomycorrhizal fungi |
| _version_ | 1878741117454778368 |
|---|---|
| author | Piñuela, Yasmine |
| author2 | Alday, Josu G. Oliach, Daniel Bolaño, Francesc Colinas, C. (Carlos) Bonet Lledos, José Antonio |
| author2_role | author author author author author |
| author_browse | Alday, Josu G. Bolaño, Francesc Bonet Lledos, José Antonio Colinas, C. (Carlos) Oliach, Daniel Piñuela, Yasmine |
| author_facet | Piñuela, Yasmine Alday, Josu G. Oliach, Daniel Bolaño, Francesc Colinas, C. (Carlos) Bonet Lledos, José Antonio |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process and the root development of Portuguese oak (Quercus faginea Lam.) seedlings destined for truffle plantations has not been determined. The main aim of this study was to shed light on the effect of bacterial inoculation on fungal root tip colonization and seedling root traits. Material and methods: We performed a co-inoculation trial using three bacteria naturally present in the T. melanosporum niche (i.e., Pseudomonas fluorescens, Pseudomonas putida, and Bacillus amyloliquefaciens) and two different bacterial inoculation times (one month and nine months after fungal inoculation) under glasshouse conditions. Results: Only P. fluorescens had a significant mycorrhizal promoter effect, increasing the truffle inoculation rates of root tips by more than 10% compared with seedlings that received non-bacterial inoculation treatments. Simultaneously, the co-inoculation of P. fluorescens with T. melanosporum improved seedling root growth parameters compared with those of seedlings that received non-bacterial inoculation treatments. The different bacterial inoculation times and applications of uninoculated bacterial growth media did not affect the root traits analyzed or the root mycorrhization rates. Conclusions: These results suggest that P. fluorescens bacteria have a potential commercial application as a treatment for truffle-inoculated seedlings to improve both seedling quality and mycorrhizal colonization under nursery conditions. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_df6ab4a76b4e59b7b60db5a02c32cbb5 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:10459.1/69758 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings Piñuela, Yasmine Alday, Josu G. Oliach, Daniel Bolaño, Francesc Colinas, C. (Carlos) Bonet Lledos, José Antonio Black truffle Truffle colonization Edible fungi cultivation Truffle orchard Truffle trees Hypogeous ectomycorrhizal fungi Research Highlights: Mycorrhizal helper bacteria (MHB) promote mycorrhization processes and are commonly found in the mycorrhizosphere of fungi, such as the edible hypogeous fungus Tuber melanosporum Vittad. Background and Objectives: The effectiveness of MHB in promoting the mycorrhization process and the root development of Portuguese oak (Quercus faginea Lam.) seedlings destined for truffle plantations has not been determined. The main aim of this study was to shed light on the effect of bacterial inoculation on fungal root tip colonization and seedling root traits. Material and methods: We performed a co-inoculation trial using three bacteria naturally present in the T. melanosporum niche (i.e., Pseudomonas fluorescens, Pseudomonas putida, and Bacillus amyloliquefaciens) and two different bacterial inoculation times (one month and nine months after fungal inoculation) under glasshouse conditions. Results: Only P. fluorescens had a significant mycorrhizal promoter effect, increasing the truffle inoculation rates of root tips by more than 10% compared with seedlings that received non-bacterial inoculation treatments. Simultaneously, the co-inoculation of P. fluorescens with T. melanosporum improved seedling root growth parameters compared with those of seedlings that received non-bacterial inoculation treatments. The different bacterial inoculation times and applications of uninoculated bacterial growth media did not affect the root traits analyzed or the root mycorrhization rates. Conclusions: These results suggest that P. fluorescens bacteria have a potential commercial application as a treatment for truffle-inoculated seedlings to improve both seedling quality and mycorrhizal colonization under nursery conditions. This research was funded by the Project INNOVATRUF (PECT El bosc, el primer recurs de l’economia verda—Fons Europeu de Desenvolupament Regional de la Unió Europea-Programa operatiu FEDER de Catalunya 2014-2020) and by the project UdL-Impuls. Y.P. acknowledges the support of University of Lleida for her contract (UdL-Impuls), J.G.A. was supported by the Ramon y Cajal fellowship (RYC-2016-20528), D.O. received support from the ‘Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya’ through the program of ‘Doctorats Industrials’, F.B.’s salary was partially funded by the Ministry of Science, Innovation and Universities through the National Agency of Research (PTA2017-14041-I) and J.A.B. benefitted from a Serra-Húnter Fellowship provided by the Generalitat of Catalunya. MDPI https://doi.org/10.3390/f11080792 http://hdl.handle.net/10459.1/69758 |
| spellingShingle | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings Piñuela, Yasmine Black truffle Truffle colonization Edible fungi cultivation Truffle orchard Truffle trees Hypogeous ectomycorrhizal fungi |
| status_str | publishedVersion |
| title | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings |
| title_full | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings |
| title_fullStr | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings |
| title_full_unstemmed | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings |
| title_short | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings |
| title_sort | Use of Inoculator Bacteria to Promote Tuber melanosporum Root Colonization and Growth on Quercus faginea Saplings |
| topic | Black truffle Truffle colonization Edible fungi cultivation Truffle orchard Truffle trees Hypogeous ectomycorrhizal fungi |
| topic_facet | Black truffle Truffle colonization Edible fungi cultivation Truffle orchard Truffle trees Hypogeous ectomycorrhizal fungi |
| url | https://doi.org/10.3390/f11080792 http://hdl.handle.net/10459.1/69758 |