Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
Incluye un fichero de datos
| Authors: | ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2013 |
| Country: | España |
| Institution: | Universidad Pública de Navarra |
| Repository: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/23536 |
| Online Access: | https://hdl.handle.net/2454/23536 |
| Access Level: | Open access |
| Keyword: | Human papillomavirus (HPV) HIV PCR-based moleculat tests Urine samples |
| _version_ | 1878741082879033344 |
|---|---|
| author | Muñoz Bobo, Marina |
| author2 | Camargo, Milena Soto de León, Sara Sánchez, Ricardo Pineda Peña, Andrea Pérez Prados, Antonio Patarroyo, Manuel Elkin Patarroyo, Manuel Alfonso |
| author2_role | author author author author author author author |
| author_browse | Camargo, Milena Muñoz Bobo, Marina Patarroyo, Manuel Alfonso Patarroyo, Manuel Elkin Pineda Peña, Andrea Pérez Prados, Antonio Soto de León, Sara Sánchez, Ricardo |
| author_facet | Muñoz Bobo, Marina Camargo, Milena Soto de León, Sara Sánchez, Ricardo Pineda Peña, Andrea Pérez Prados, Antonio Patarroyo, Manuel Elkin Patarroyo, Manuel Alfonso |
| author_role | author |
| collection | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| description | Incluye un fichero de datos |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_dec3b9bec4f0ffd081826d2ca3ebf4d4 |
| institution | Universidad Pública de Navarra |
| instname_str | Universidad Pública de Navarra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:academica-e.unavarra.es:2454/23536 |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| reponame_str | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| spelling | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women Muñoz Bobo, Marina Camargo, Milena Soto de León, Sara Sánchez, Ricardo Pineda Peña, Andrea Pérez Prados, Antonio Patarroyo, Manuel Elkin Patarroyo, Manuel Alfonso Human papillomavirus (HPV) HIV PCR-based moleculat tests Urine samples Incluye un fichero de datos Human papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5 /GP6 , MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women. Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples. HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in urine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples. This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples. The authors are grateful to the Basque Cooperation Agency for Development and the Spanish Agency for International Development Cooperation (AECID) for supporting and financing this project. American Society for Microbiology https://hdl.handle.net/2454/23536 |
| spellingShingle | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women Muñoz Bobo, Marina Human papillomavirus (HPV) HIV PCR-based moleculat tests Urine samples |
| status_str | publishedVersion |
| title | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
| title_full | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
| title_fullStr | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
| title_full_unstemmed | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
| title_short | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
| title_sort | Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women |
| topic | Human papillomavirus (HPV) HIV PCR-based moleculat tests Urine samples |
| topic_facet | Human papillomavirus (HPV) HIV PCR-based moleculat tests Urine samples |
| url | https://hdl.handle.net/2454/23536 |