Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
Purpose The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. Methods This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a Univers...
| Autores: | ; ; ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/194964 |
| Acceso en línea: | https://hdl.handle.net/2445/194964 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Nodrissons Microbiota Tos ferina Rinofaringe Infants Whooping cough Nasopharynx |
| _version_ | 1878740921869139968 |
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| author | Rocafort, Muntsa |
| author2 | Henares, Desiree Brotons, Pedro Barrabeig i Fabregat, Irene Launes Montaña, Cristian Merdrignac, Lore Valenciano, Marta Domínguez García, Àngela Godoy, Pere Muñoz-Almagro, Carmen |
| author2_role | author author author author author author author author author |
| author_browse | Barrabeig i Fabregat, Irene Brotons, Pedro Domínguez García, Àngela Godoy, Pere Henares, Desiree Launes Montaña, Cristian Merdrignac, Lore Muñoz-Almagro, Carmen Rocafort, Muntsa Valenciano, Marta |
| author_facet | Rocafort, Muntsa Henares, Desiree Brotons, Pedro Barrabeig i Fabregat, Irene Launes Montaña, Cristian Merdrignac, Lore Valenciano, Marta Domínguez García, Àngela Godoy, Pere Muñoz-Almagro, Carmen |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Purpose The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. Methods This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. Results Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy contro |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_dbcb3f84e1dcedb2a6fca4b9e966e9a2 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/194964 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher | Public Library of Science (PLoS) |
| publisherStr | Public Library of Science (PLoS) |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study Rocafort, Muntsa Henares, Desiree Brotons, Pedro Barrabeig i Fabregat, Irene Launes Montaña, Cristian Merdrignac, Lore Valenciano, Marta Domínguez García, Àngela Godoy, Pere Muñoz-Almagro, Carmen Nodrissons Microbiota Tos ferina Rinofaringe Infants Whooping cough Nasopharynx Purpose The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. Methods This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. Results Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy contro Public Library of Science (PLoS) https://hdl.handle.net/2445/194964 |
| spellingShingle | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study Rocafort, Muntsa Nodrissons Microbiota Tos ferina Rinofaringe Infants Whooping cough Nasopharynx |
| status_str | publishedVersion |
| title | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study |
| title_full | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study |
| title_fullStr | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study |
| title_full_unstemmed | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study |
| title_short | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study |
| title_sort | Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study |
| topic | Nodrissons Microbiota Tos ferina Rinofaringe Infants Whooping cough Nasopharynx |
| topic_facet | Nodrissons Microbiota Tos ferina Rinofaringe Infants Whooping cough Nasopharynx |
| url | https://hdl.handle.net/2445/194964 |