The incC sequence is required for R27 plasmid stability
IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2016 |
| Country: | España |
| Institution: | Universidad de Barcelona |
| Repository: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/112611 |
| Online Access: | https://hdl.handle.net/2445/112611 |
| Access Level: | Open access |
| Keyword: | Seqüència d'aminoàcids Plasmidis Amino acid sequence Plasmids |
| _version_ | 1878740587237081088 |
|---|---|
| author | Tassinari, Eleonora |
| author2 | Aznar Vicente, Sonia Urcola, Imanol Prieto Durán, Alejandro Hüttener Queiroz, Mário Juárez Giménez, Antonio |
| author2_role | author author author author author |
| author_browse | Aznar Vicente, Sonia Hüttener Queiroz, Mário Juárez Giménez, Antonio Prieto Durán, Alejandro Tassinari, Eleonora Urcola, Imanol |
| author_facet | Tassinari, Eleonora Aznar Vicente, Sonia Urcola, Imanol Prieto Durán, Alejandro Hüttener Queiroz, Mário Juárez Giménez, Antonio |
| author_role | author |
| collection | Dipòsit Digital de la UB |
| description | IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_d5c60ecc7eaeea8e5158ae0d2c63ddbf |
| institution | Universidad de Barcelona |
| instname_str | Universidad de Barcelona |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:diposit.ub.edu:2445/112611 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| reponame_str | Dipòsit Digital de la UB |
| spelling | The incC sequence is required for R27 plasmid stability Tassinari, Eleonora Aznar Vicente, Sonia Urcola, Imanol Prieto Durán, Alejandro Hüttener Queiroz, Mário Juárez Giménez, Antonio Seqüència d'aminoàcids Plasmidis Amino acid sequence Plasmids IncHI plasmids account for multiple antimicrobial resistance in Salmonella and other enterobacterial genera. These plasmids are generally very stable in their bacterial hosts. R27 is the archetype of IncHI1 plasmids. A high percentage of the R27-encoded open reading frames (ORFs) (66.7%) do not show similarity to any known ORFs. We performed a deletion analysis of all non-essential R27 DNA sequences to search for hitherto non-identified plasmid functions that might be required for plasmid stability. We report the identification of a short DNA sequence (incC) that is essential for R27 stability. That region contains several repeats (incC repeats), belongs to one of the three-plasmid replicons (R27 FIA-like) and is targeted by the R27 E protein. Deletion of the incC sequence drastically reduces R27 stability both in Escherichia coli and in Salmonella, the effect being more pronounced in this latter species. Interfering with incC-E protein interaction must lead to a reduced IncHI1 plasmid stability, and may represent a new approach to combat antimicrobial resistance. Frontiers Media https://hdl.handle.net/2445/112611 |
| spellingShingle | The incC sequence is required for R27 plasmid stability Tassinari, Eleonora Seqüència d'aminoàcids Plasmidis Amino acid sequence Plasmids |
| status_str | publishedVersion |
| title | The incC sequence is required for R27 plasmid stability |
| title_full | The incC sequence is required for R27 plasmid stability |
| title_fullStr | The incC sequence is required for R27 plasmid stability |
| title_full_unstemmed | The incC sequence is required for R27 plasmid stability |
| title_short | The incC sequence is required for R27 plasmid stability |
| title_sort | The incC sequence is required for R27 plasmid stability |
| topic | Seqüència d'aminoàcids Plasmidis Amino acid sequence Plasmids |
| topic_facet | Seqüència d'aminoàcids Plasmidis Amino acid sequence Plasmids |
| url | https://hdl.handle.net/2445/112611 |