Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid
A low cost microreactor was developed by stable immobilization of catechol-1,2-dioxygenase for the flow biocatalysis of catechol to produce cis,cis-muconic acid, a direct precursor of industrially relevant adipic acid. The microreactors of polydimethylsiloxane were manufactured with different design...
| Authors: | ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2025 |
| Country: | España |
| Institution: | Universidad Pública de Navarra |
| Repository: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/55618 |
| Online Access: | https://hdl.handle.net/2454/55618 |
| Access Level: | Open access |
| Keyword: | Flow biocatalysis PDMS microreactors cis,cis-muconic acid Enzyme immobilization Catechol-1,2-dioxygenase |
| _version_ | 1878740572981690368 |
|---|---|
| author | Rosso, Samuele |
| author2 | Almazán, Fernando Pellejero, Ismael Gandía Pascual, Luis Dezzani, Sabrina Barbieri, Lisa Gilardi, Gianfranco Valetti, Francesca Di Nardo, Giovanna |
| author2_role | author author author author author author author author |
| author_browse | Almazán, Fernando Barbieri, Lisa Dezzani, Sabrina Di Nardo, Giovanna Gandía Pascual, Luis Gilardi, Gianfranco Pellejero, Ismael Rosso, Samuele Valetti, Francesca |
| author_facet | Rosso, Samuele Almazán, Fernando Pellejero, Ismael Gandía Pascual, Luis Dezzani, Sabrina Barbieri, Lisa Gilardi, Gianfranco Valetti, Francesca Di Nardo, Giovanna |
| author_role | author |
| collection | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| description | A low cost microreactor was developed by stable immobilization of catechol-1,2-dioxygenase for the flow biocatalysis of catechol to produce cis,cis-muconic acid, a direct precursor of industrially relevant adipic acid. The microreactors of polydimethylsiloxane were manufactured with different designs by straightforward, cost- effective soft lithography techniques, and were tested with different immobilization strategies focused on preserving enzyme catalytic activity and stability over time. The best immobilization strategy was covalent immobilization by amino-silanization followed by glutaraldehyde linker grafting. The biocatalytic activity of the immobilized enzyme was assessed by measuring muconic acid production through the flow of 50 µM catechol at room temperature. Catechol conversion rates of up to 95 % were achieved over a 5-hour period, and volumes of up to 180 mL were processed using only 0.32 nmol of immobilized protein, resulting in the production of 1.33 mg⋅L 1⋅h 1. This work demonstrates the feasibility of using microreactor-based flow biocatalysis for sustainable muconic acid production and highlights its promising application at industrial scale. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_d5805f94a4c7be4027aa856416bda8cd |
| institution | Universidad Pública de Navarra |
| instname_str | Universidad Pública de Navarra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:academica-e.unavarra.es:2454/55618 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| spelling | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid Rosso, Samuele Almazán, Fernando Pellejero, Ismael Gandía Pascual, Luis Dezzani, Sabrina Barbieri, Lisa Gilardi, Gianfranco Valetti, Francesca Di Nardo, Giovanna Flow biocatalysis PDMS microreactors cis,cis-muconic acid Enzyme immobilization Catechol-1,2-dioxygenase A low cost microreactor was developed by stable immobilization of catechol-1,2-dioxygenase for the flow biocatalysis of catechol to produce cis,cis-muconic acid, a direct precursor of industrially relevant adipic acid. The microreactors of polydimethylsiloxane were manufactured with different designs by straightforward, cost- effective soft lithography techniques, and were tested with different immobilization strategies focused on preserving enzyme catalytic activity and stability over time. The best immobilization strategy was covalent immobilization by amino-silanization followed by glutaraldehyde linker grafting. The biocatalytic activity of the immobilized enzyme was assessed by measuring muconic acid production through the flow of 50 µM catechol at room temperature. Catechol conversion rates of up to 95 % were achieved over a 5-hour period, and volumes of up to 180 mL were processed using only 0.32 nmol of immobilized protein, resulting in the production of 1.33 mg⋅L 1⋅h 1. This work demonstrates the feasibility of using microreactor-based flow biocatalysis for sustainable muconic acid production and highlights its promising application at industrial scale. Financial support from University of Turin “GRANT for INTERNATIONALIZATION - GFI 2022” and the Spanish Ministerio de Ciencia e Innovación and Spanish Agencia Estatal de Investigación (grants PID2019–106687RJ-I00, PID2021–127265OB-C21) is gratefully acknowledged. Open access funding provided by University of Torino. Elsevier https://hdl.handle.net/2454/55618 |
| spellingShingle | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid Rosso, Samuele Flow biocatalysis PDMS microreactors cis,cis-muconic acid Enzyme immobilization Catechol-1,2-dioxygenase |
| status_str | publishedVersion |
| title | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid |
| title_full | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid |
| title_fullStr | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid |
| title_full_unstemmed | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid |
| title_short | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid |
| title_sort | Development of a flow microreactor with immobilized catechol-1,2-dioxygenase for the biocatalytic production of cis,cis-muconic acid |
| topic | Flow biocatalysis PDMS microreactors cis,cis-muconic acid Enzyme immobilization Catechol-1,2-dioxygenase |
| topic_facet | Flow biocatalysis PDMS microreactors cis,cis-muconic acid Enzyme immobilization Catechol-1,2-dioxygenase |
| url | https://hdl.handle.net/2454/55618 |