Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells
Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of...
| Authors: | ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/195645 |
| Online Access: | https://hdl.handle.net/2445/195645 |
| Access Level: | Open access |
| Keyword: | Materials biomèdics Impressió 3D Àcid hialurònic Diferenciació de productes Regeneració del sistema nerviós Biomedical materials Three-dimensional printing Hyaluronic acid Product differentiation Nervous system regeneration |
| _version_ | 1878740513464516609 |
|---|---|
| author | Pereira, Inês Sousa |
| author2 | López Martínez, María José Villasante, Aranzazu Introna, Clelia Tornero, Daniel Canals i Coll, Josep M. Samitier i Martí, Josep |
| author2_role | author author author author author author |
| author_browse | Canals i Coll, Josep M. Introna, Clelia López Martínez, María José Pereira, Inês Sousa Samitier i Martí, Josep Tornero, Daniel Villasante, Aranzazu |
| author_facet | Pereira, Inês Sousa López Martínez, María José Villasante, Aranzazu Introna, Clelia Tornero, Daniel Canals i Coll, Josep M. Samitier i Martí, Josep |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_d467ee339f4ce301af2dde668cd00a90 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/195645 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells Pereira, Inês Sousa López Martínez, María José Villasante, Aranzazu Introna, Clelia Tornero, Daniel Canals i Coll, Josep M. Samitier i Martí, Josep Materials biomèdics Impressió 3D Àcid hialurònic Diferenciació de productes Regeneració del sistema nerviós Biomedical materials Three-dimensional printing Hyaluronic acid Product differentiation Nervous system regeneration Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge. Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture. Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging. Frontiers Media https://hdl.handle.net/2445/195645 |
| spellingShingle | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells Pereira, Inês Sousa Materials biomèdics Impressió 3D Àcid hialurònic Diferenciació de productes Regeneració del sistema nerviós Biomedical materials Three-dimensional printing Hyaluronic acid Product differentiation Nervous system regeneration |
| status_str | publishedVersion |
| title | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
| title_full | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
| title_fullStr | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
| title_full_unstemmed | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
| title_short | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
| title_sort | Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells |
| topic | Materials biomèdics Impressió 3D Àcid hialurònic Diferenciació de productes Regeneració del sistema nerviós Biomedical materials Three-dimensional printing Hyaluronic acid Product differentiation Nervous system regeneration |
| topic_facet | Materials biomèdics Impressió 3D Àcid hialurònic Diferenciació de productes Regeneració del sistema nerviós Biomedical materials Three-dimensional printing Hyaluronic acid Product differentiation Nervous system regeneration |
| url | https://hdl.handle.net/2445/195645 |