Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model
Purpose: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical size alveolar ridge defects. Materials and methods: In 18 dogs, four dehiscence-type and critical size defects were created surgically in the edentulous a...
| Authors: | ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Accepted version |
| Publication date: | 2016 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/183026 |
| Online Access: | https://hdl.handle.net/2445/183026 |
| Access Level: | Open access |
| Keyword: | Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| _version_ | 1878740478007967744 |
|---|---|
| author | Alvira González, Joaquín |
| author2 | Sánchez Garcés, Ma. Ángeles Barbany, Joan Ramon Reina del Pozo, Manuel Müller Sánchez, Claudia Alejandra Gay Escoda, Cosme |
| author2_role | author author author author author |
| author_browse | Alvira González, Joaquín Barbany, Joan Ramon Gay Escoda, Cosme Müller Sánchez, Claudia Alejandra Reina del Pozo, Manuel Sánchez Garcés, Ma. Ángeles |
| author_facet | Alvira González, Joaquín Sánchez Garcés, Ma. Ángeles Barbany, Joan Ramon Reina del Pozo, Manuel Müller Sánchez, Claudia Alejandra Gay Escoda, Cosme |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Purpose: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical size alveolar ridge defects. Materials and methods: In 18 dogs, four dehiscence-type and critical size defects were created surgically in the edentulous alveolar ridge. Defects were randomly regenerated using biomaterials coated with particulate ß-tricalcium phosphate (ß-TCP), ß-TCP with fibronectin (Fn) (ß-TCP-Fn), and ß-TCP with a combination of Fn and autologous adipose-derived stem cells (ADSCs) (ß-TCP-Fn-ADSCs), leaving one defect as control. The animals were divided into three groups according to the time of euthanasia (1, 2, or 3 months of healing). Results: At the time of sacrifice, statistically significant differences between the four types of defects in the total area of bone regeneration, percentage of neoformed bone matrix, medullary space, or contact between particulate biomaterial and neoformed bone matrix were not found. All defects showed a significant increase in neoformed bone matrix as sacrifice was delayed, but a uniform pattern was not followed. Only defects treated with ß-TCP-Fn-ADSCs showed a significant increase in the bone regeneration area when animals sacrificed at 3 months were compared to those sacrificed at 1 month (P = .006). Conclusion: The use of ADSCs in bone regeneration processes of critical size defects of the alveolar ridge did not entail an advantage regarding greater bone regeneration as compared with other biomaterials. However, the use of ß-TCP coated with a combination of Fn and ADSCs appeared to favor stabilization of the regenerated area, allowing a more efficient maintenance of the space at 3 months of healing. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_d3c19e171ca82da6565aab5ae88baf85 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/183026 |
| publishDate | 2016 |
| publishDateSort | 2016 |
| publisher | Quintessence Publishing Group |
| publisherStr | Quintessence Publishing Group |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model Alvira González, Joaquín Sánchez Garcés, Ma. Ángeles Barbany, Joan Ramon Reina del Pozo, Manuel Müller Sánchez, Claudia Alejandra Gay Escoda, Cosme Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones Purpose: To assess bone regeneration potential of a fibronectin- and adipose-derived stem cell-covered ceramic biomaterial in three-wall critical size alveolar ridge defects. Materials and methods: In 18 dogs, four dehiscence-type and critical size defects were created surgically in the edentulous alveolar ridge. Defects were randomly regenerated using biomaterials coated with particulate ß-tricalcium phosphate (ß-TCP), ß-TCP with fibronectin (Fn) (ß-TCP-Fn), and ß-TCP with a combination of Fn and autologous adipose-derived stem cells (ADSCs) (ß-TCP-Fn-ADSCs), leaving one defect as control. The animals were divided into three groups according to the time of euthanasia (1, 2, or 3 months of healing). Results: At the time of sacrifice, statistically significant differences between the four types of defects in the total area of bone regeneration, percentage of neoformed bone matrix, medullary space, or contact between particulate biomaterial and neoformed bone matrix were not found. All defects showed a significant increase in neoformed bone matrix as sacrifice was delayed, but a uniform pattern was not followed. Only defects treated with ß-TCP-Fn-ADSCs showed a significant increase in the bone regeneration area when animals sacrificed at 3 months were compared to those sacrificed at 1 month (P = .006). Conclusion: The use of ADSCs in bone regeneration processes of critical size defects of the alveolar ridge did not entail an advantage regarding greater bone regeneration as compared with other biomaterials. However, the use of ß-TCP coated with a combination of Fn and ADSCs appeared to favor stabilization of the regenerated area, allowing a more efficient maintenance of the space at 3 months of healing. Quintessence Publishing Group https://hdl.handle.net/2445/183026 |
| spellingShingle | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model Alvira González, Joaquín Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| status_str | acceptedVersion |
| title | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_full | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_fullStr | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_full_unstemmed | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_short | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| title_sort | Assessment of bone-regeneration using adipose-derived stem cells in critical-size alveolar ridge defects: an experimental study in a dog model |
| topic | Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| topic_facet | Cèl·lules mare Glicoproteïnes Regeneració (Biologia) Ossos Stem cells Glycoproteins Regeneration (Biology) Bones |
| url | https://hdl.handle.net/2445/183026 |