Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties
Herein, a versatile bilayersystem, composed by a polypropylene(PP)mesh and a covalently bonded poly(N-isopropylacrylamide) (PNIPAAm) hydrogel, is reported. The cell adhesion mechanism was successfully modulated by controlling the architecture of the hydrogel in terms of duration of PNIPAAm graftingt...
| Authors: | ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Universitat Politècnica de Catalunya (UPC) |
| Repository: | UPCommons. Portal del coneixement obert de la UPC |
| Language: | English |
| OAI Identifier: | oai:upcommons.upc.edu:2117/174604 |
| Online Access: | https://hdl.handle.net/2117/174604 https://dx.doi.org/10.1039/c9tb02537e |
| Access Level: | Open access |
| Keyword: | Biomedical materials Materials biomèdics Àrees temàtiques de la UPC::Enginyeria química |
| _version_ | 1878740335089156096 |
|---|---|
| author | Lanzalaco, Sonia|||0000-0002-8604-5095 |
| author2 | Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Turón Dols, Pau Weis, Christine Estrany Coda, Francesc|||0000-0002-2696-1489 Alemán Llansó, Carlos|||0000-0003-4462-6075 Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696 |
| author2_role | author author author author author author |
| author_browse | Alemán Llansó, Carlos|||0000-0003-4462-6075 Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696 Estrany Coda, Francesc|||0000-0002-2696-1489 Lanzalaco, Sonia|||0000-0002-8604-5095 Turón Dols, Pau Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Weis, Christine |
| author_facet | Lanzalaco, Sonia|||0000-0002-8604-5095 Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Turón Dols, Pau Weis, Christine Estrany Coda, Francesc|||0000-0002-2696-1489 Alemán Llansó, Carlos|||0000-0003-4462-6075 Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696 |
| author_role | author |
| collection | UPCommons. Portal del coneixement obert de la UPC |
| description | Herein, a versatile bilayersystem, composed by a polypropylene(PP)mesh and a covalently bonded poly(N-isopropylacrylamide) (PNIPAAm) hydrogel, is reported. The cell adhesion mechanism was successfully modulated by controlling the architecture of the hydrogel in terms of duration of PNIPAAm graftingtime, crosslinker content, and temperature of material exposure in PBS solutions (belowandabove the LCST of PNIPAAm). The best in vitroresults with fibroblast (COS-1) and epithelial (MCF-7) cells was obtained with a mesh modified with porous iPP-g-PNIPAAm bilayer system, prepared via PNIPAAm grafting for 2 h at the lowest N,N'-methylene bis(acrylamide) (MBA)concentration (1 mM). Under these conditions, the detachment of the fibroblast-like cells was 50% lower than that of the control, after 7 days of cell incubation, which represents a high de-adhesionof cellsin a short period. Moreover, the whole system showed an excellent stability in dry or wet media, proving that the thermosensitive hydrogel was well adhered to the polymer surface, after PP fibreactivation by cold plasma. This study opens new insights on the development of anti-adherent meshes for abdominal hernia repairs. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_d130077e2473d9a59ccd96f30b59d5b3 |
| institution | Universitat Politècnica de Catalunya (UPC) |
| instname_str | Universitat Politècnica de Catalunya (UPC) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:upcommons.upc.edu:2117/174604 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Royal Society of Chemistry (RSC) |
| publisherStr | Royal Society of Chemistry (RSC) |
| reponame_str | UPCommons. Portal del coneixement obert de la UPC |
| spelling | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties Lanzalaco, Sonia|||0000-0002-8604-5095 Valle Mendoza, Luis Javier del|||0000-0001-9916-1741 Turón Dols, Pau Weis, Christine Estrany Coda, Francesc|||0000-0002-2696-1489 Alemán Llansó, Carlos|||0000-0003-4462-6075 Armelín Diggroc, Elaine Aparecida|||0000-0002-0658-7696 Biomedical materials Materials biomèdics Àrees temàtiques de la UPC::Enginyeria química Herein, a versatile bilayersystem, composed by a polypropylene(PP)mesh and a covalently bonded poly(N-isopropylacrylamide) (PNIPAAm) hydrogel, is reported. The cell adhesion mechanism was successfully modulated by controlling the architecture of the hydrogel in terms of duration of PNIPAAm graftingtime, crosslinker content, and temperature of material exposure in PBS solutions (belowandabove the LCST of PNIPAAm). The best in vitroresults with fibroblast (COS-1) and epithelial (MCF-7) cells was obtained with a mesh modified with porous iPP-g-PNIPAAm bilayer system, prepared via PNIPAAm grafting for 2 h at the lowest N,N'-methylene bis(acrylamide) (MBA)concentration (1 mM). Under these conditions, the detachment of the fibroblast-like cells was 50% lower than that of the control, after 7 days of cell incubation, which represents a high de-adhesionof cellsin a short period. Moreover, the whole system showed an excellent stability in dry or wet media, proving that the thermosensitive hydrogel was well adhered to the polymer surface, after PP fibreactivation by cold plasma. This study opens new insights on the development of anti-adherent meshes for abdominal hernia repairs. Peer Reviewed Royal Society of Chemistry (RSC) https://hdl.handle.net/2117/174604 https://dx.doi.org/10.1039/c9tb02537e |
| spellingShingle | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties Lanzalaco, Sonia|||0000-0002-8604-5095 Biomedical materials Materials biomèdics Àrees temàtiques de la UPC::Enginyeria química |
| title | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties |
| title_full | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties |
| title_fullStr | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties |
| title_full_unstemmed | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties |
| title_short | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties |
| title_sort | Polypropylene mesh for hernia repair with controllable cell adhesion/de-adhesion properties |
| topic | Biomedical materials Materials biomèdics Àrees temàtiques de la UPC::Enginyeria química |
| topic_facet | Biomedical materials Materials biomèdics Àrees temàtiques de la UPC::Enginyeria química |
| url | https://hdl.handle.net/2117/174604 https://dx.doi.org/10.1039/c9tb02537e |