Pneumocystis jirovecii among patients with cystic fibrosis and their household members
We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10...
| Autores: | ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/141093 |
| Acceso en línea: | https://hdl.handle.net/11441/141093 https://doi.org/10.1093/mmy/myab010 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Pneumocystis jirovecii disease transmission cystic fibrosis polymerase chain reaction |
| _version_ | 1878740279297572864 |
|---|---|
| author | Morilla Romero de la Osa, Rubén |
| author2 | Medrano Ortega, Francisco Javier Calzada, Ana Quintana, Esther Campano, Elena Friaza, Vicente Calderón Sandubete, Enrique José Horra Padilla, Carmen de la |
| author2_role | author author author author author author author |
| author_browse | Calderón Sandubete, Enrique José Calzada, Ana Campano, Elena Friaza, Vicente Horra Padilla, Carmen de la Medrano Ortega, Francisco Javier Morilla Romero de la Osa, Rubén Quintana, Esther |
| author_facet | Morilla Romero de la Osa, Rubén Medrano Ortega, Francisco Javier Calzada, Ana Quintana, Esther Campano, Elena Friaza, Vicente Calderón Sandubete, Enrique José Horra Padilla, Carmen de la |
| author_role | author |
| collection | idUS. Depósito de Investigación de la Universidad de Sevilla |
| description | We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_d03edf593696c9da4dc7b1a68e863e50 |
| institution | Universidad de Sevilla (US) |
| instname_str | Universidad de Sevilla (US) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:idus.us.es:11441/141093 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | idUS. Depósito de Investigación de la Universidad de Sevilla |
| spelling | Pneumocystis jirovecii among patients with cystic fibrosis and their household members Morilla Romero de la Osa, Rubén Medrano Ortega, Francisco Javier Calzada, Ana Quintana, Esther Campano, Elena Friaza, Vicente Calderón Sandubete, Enrique José Horra Padilla, Carmen de la Pneumocystis jirovecii disease transmission cystic fibrosis polymerase chain reaction We conducted a pilot study of patients with cystic fibrosis (CF) to assess intra-family transmission of P. jirovecii and compare it with data on other prevalent pathogens such as P. aeruginosa and S. pneumoniae, in which respiratory transmission has already been documented. Oral swab samples from 10 patients with CF and 15 household members were collected at baseline and 2 weeks later. P. aeruginosa and S. pneumoniae were assessed using standardized culture methods and PCR, and P. jirovecii was assessed using real and nested PCR, genotyping the positive samples by direct sequencing. P. aeruginosa cultures were positive for 7/10 (70%) of patients with CF at baseline and was identified by PCR in 8/10 (80%) of cases at baseline and 2 weeks later. S. pneumoniae cultures were negative for all patients, but the microorganism was identified by PCR in two cases. P. jirovecii was detected by real time and nested PCR in 5/10 (50%) of the patients at the two time points. In the household members, P. aeruginosa and P. jirovecii were identified in 7/15 (46.7%), and S. pneumoniae was identified in 8/15 (53,3%). The concordance of positive or negative pairs of patients with CF and their household members was 33.3% (5/15) for P. aeruginosa, 46.7% (7/15) for S. pneumonia and 93.3% (14/15) for P. jirovecii. The concordance for P. jirovecii genotypes among five pairs with available genotype was 100%. This study suggests for the first time the possible transmission of Pneumocystis in the home of patients with CF, indicating that patients and their household members are reservoirs and possible sources of infection. Oxford University Press https://hdl.handle.net/11441/141093 https://doi.org/10.1093/mmy/myab010 |
| spellingShingle | Pneumocystis jirovecii among patients with cystic fibrosis and their household members Morilla Romero de la Osa, Rubén Pneumocystis jirovecii disease transmission cystic fibrosis polymerase chain reaction |
| status_str | publishedVersion |
| title | Pneumocystis jirovecii among patients with cystic fibrosis and their household members |
| title_full | Pneumocystis jirovecii among patients with cystic fibrosis and their household members |
| title_fullStr | Pneumocystis jirovecii among patients with cystic fibrosis and their household members |
| title_full_unstemmed | Pneumocystis jirovecii among patients with cystic fibrosis and their household members |
| title_short | Pneumocystis jirovecii among patients with cystic fibrosis and their household members |
| title_sort | Pneumocystis jirovecii among patients with cystic fibrosis and their household members |
| topic | Pneumocystis jirovecii disease transmission cystic fibrosis polymerase chain reaction |
| topic_facet | Pneumocystis jirovecii disease transmission cystic fibrosis polymerase chain reaction |
| url | https://hdl.handle.net/11441/141093 https://doi.org/10.1093/mmy/myab010 |