PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue
[EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment int...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2022 |
| Country: | España |
| Institution: | Universitat Politècnica de València (UPV) |
| Repository: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Language: | English |
| OAI Identifier: | oai:riunet.upv.es:10251/193324 |
| Online Access: | https://riunet.upv.es/handle/10251/193324 |
| Access Level: | Open access |
| Keyword: | ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| _version_ | 1878740232886550528 |
|---|---|
| author | Pizzio, Gaston A. |
| author2 | Mayordomo, Cristian Lozano-Juste, Jorge Vazquez-Vilar, Marta Kaminski, Kacper P. Ivanov, Nikolai V. Estevez ,Juan C. Rivera-Moreno, Maria Albert, Armando Rodríguez, Pedro L. García-Carpintero-Burgos, Víctor Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente |
| author2_role | author author author author author author author author author author author author |
| author_browse | Albert, Armando Estevez ,Juan C. García-Carpintero-Burgos, Víctor Ivanov, Nikolai V. Kaminski, Kacper P. Lozano-Juste, Jorge Mayordomo, Cristian Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente Pizzio, Gaston A. Rivera-Moreno, Maria Rodríguez, Pedro L. Vazquez-Vilar, Marta |
| author_facet | Pizzio, Gaston A. Mayordomo, Cristian Lozano-Juste, Jorge Vazquez-Vilar, Marta Kaminski, Kacper P. Ivanov, Nikolai V. Estevez ,Juan C. Rivera-Moreno, Maria Albert, Armando Rodríguez, Pedro L. García-Carpintero-Burgos, Víctor Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente |
| author_role | author |
| collection | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| description | [EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment interactions is quite challenging in polyploid plants because of the high number of genes in the PYR/PYL/RCAR ABA receptor family. Nicotiana benthamiana is a biotechnological crop amenable to genome editing, and given the importance of ABA signaling in coping with drought stress, we initiated the analysis of its 23-member family of ABA receptors through multiplex CRISPR/Cas9-mediated editing. We generated several high-order mutants impaired in NbPYL1-like and NbPYL8-like receptors, which showed certain insensitivity to ABA for inhibition of seedling establishment, growth, and development of shoot and lateral roots as well as reduced sensitivity to the PYL1-agonist cyanabactin (CB). However, in these high-order mutants, regulation of transpiration was not affected and was responsive to ABA treatment. This reveals a robust and redundant control of transpiration in this allotetraploid plant that probably reflects its origin from the extreme habitat of central Australia. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_cf742e5ef1d9be6a7b8cebd63daa956e |
| institution | Universitat Politècnica de València (UPV) |
| instname_str | Universitat Politècnica de València (UPV) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:riunet.upv.es:10251/193324 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| spelling | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue Pizzio, Gaston A. Mayordomo, Cristian Lozano-Juste, Jorge Vazquez-Vilar, Marta Kaminski, Kacper P. Ivanov, Nikolai V. Estevez ,Juan C. Rivera-Moreno, Maria Albert, Armando Rodríguez, Pedro L. García-Carpintero-Burgos, Víctor Nebauer, Sergio G.|||0000-0001-7978-6680 Orzáez Calatayud, Diego Vicente ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible [EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of those prevailing abscisic acid (ABA) receptors that mediate plant-environment interactions is quite challenging in polyploid plants because of the high number of genes in the PYR/PYL/RCAR ABA receptor family. Nicotiana benthamiana is a biotechnological crop amenable to genome editing, and given the importance of ABA signaling in coping with drought stress, we initiated the analysis of its 23-member family of ABA receptors through multiplex CRISPR/Cas9-mediated editing. We generated several high-order mutants impaired in NbPYL1-like and NbPYL8-like receptors, which showed certain insensitivity to ABA for inhibition of seedling establishment, growth, and development of shoot and lateral roots as well as reduced sensitivity to the PYL1-agonist cyanabactin (CB). However, in these high-order mutants, regulation of transpiration was not affected and was responsive to ABA treatment. This reveals a robust and redundant control of transpiration in this allotetraploid plant that probably reflects its origin from the extreme habitat of central Australia. This research was supported by grant PID2020-113100RB (P.L.R.) and PID2020-119805RB (A.A.) funded by MCIN/AEI/10.13039/501100011033 and by Newcotiana H2020 760331 (D.O.). MDPI https://riunet.upv.es/handle/10251/193324 |
| spellingShingle | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue Pizzio, Gaston A. ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| title | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_full | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_fullStr | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_full_unstemmed | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_short | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| title_sort | PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue |
| topic | ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| topic_facet | ABA receptors Gene editing CRISPR Cas9 Nicotiana benthamiana Biotechnological crop Drought Multiplex mutations ABA sensitivity,tetraploid,extremophile FISIOLOGIA VEGETAL 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible |
| url | https://riunet.upv.es/handle/10251/193324 |