Lipid alterations in lipid rafts from alzheimer's disease human brain cortex
Lipid rafts are membrane microdomains intimately associated with cell signaling. These biochemical microstructures are characterized by their high contents of sphingolipids, cholesterol and saturated fatty acids and a reduced content of polyunsaturated fatty acids (PUFA). Here, we have purified lipi...
| Authors: | ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2010 |
| Country: | España |
| Institution: | Universidad de Barcelona |
| Repository: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/154913 |
| Online Access: | https://hdl.handle.net/2445/154913 |
| Access Level: | Open access |
| Keyword: | Lípids Escorça cerebral Malaltia d'Alzheimer Lipids Cerebral cortex Alzheimer's disease |
| _version_ | 1878740094963154944 |
|---|---|
| author | Martín, Virginia |
| author2 | Fabelo, Noemí Santpere Baró, Gabriel Puig Martorell, Berta Marín, Raquel Ferrer, Isidro (Ferrer Abizanda) Díaz, Mario |
| author2_role | author author author author author author |
| author_browse | Díaz, Mario Fabelo, Noemí Ferrer, Isidro (Ferrer Abizanda) Martín, Virginia Marín, Raquel Puig Martorell, Berta Santpere Baró, Gabriel |
| author_facet | Martín, Virginia Fabelo, Noemí Santpere Baró, Gabriel Puig Martorell, Berta Marín, Raquel Ferrer, Isidro (Ferrer Abizanda) Díaz, Mario |
| author_role | author |
| collection | Dipòsit Digital de la UB |
| description | Lipid rafts are membrane microdomains intimately associated with cell signaling. These biochemical microstructures are characterized by their high contents of sphingolipids, cholesterol and saturated fatty acids and a reduced content of polyunsaturated fatty acids (PUFA). Here, we have purified lipid rafts of human frontal brain cortex from normal and Alzheimer's disease (AD) and characterized their biochemical lipid composition. The results revealed that lipid rafts from AD brains exhibit aberrant lipid profiles compared to healthy brains. In particular, lipid rafts from AD brains displayed abnormally low levels of n-3 long chain polyunsaturated fatty acids (LCPUFA, mainly 22:6n-3, docosahexaenoic acid) and monoenes (mainly 18:1n-9, oleic acid), as well as reduced unsaturation and peroxidability indexes. Also, multiple relationships between phospholipids and fatty acids were altered in AD lipid rafts. Importantly, no changes were observed in the mole percentage of lipid classes and fatty acids in rafts from normal brains throughout the lifespan (24-85 years). These indications point to the existence of homeostatic mechanisms preserving lipid raft status in normal frontal cortex. The disruption of such mechanisms in AD brains leads to a considerable increase in lipid raft order and viscosity, which may explain the alterations in lipid raft signaling observed in AD. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_cd5f9205a1e4e0dc2cb2821aeddfa2c0 |
| institution | Universidad de Barcelona |
| instname_str | Universidad de Barcelona |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:diposit.ub.edu:2445/154913 |
| publishDate | 2010 |
| publishDateSort | 2010 |
| publisher | IOS Press |
| publisherStr | IOS Press |
| reponame_str | Dipòsit Digital de la UB |
| spelling | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex Martín, Virginia Fabelo, Noemí Santpere Baró, Gabriel Puig Martorell, Berta Marín, Raquel Ferrer, Isidro (Ferrer Abizanda) Díaz, Mario Lípids Escorça cerebral Malaltia d'Alzheimer Lipids Cerebral cortex Alzheimer's disease Lipid rafts are membrane microdomains intimately associated with cell signaling. These biochemical microstructures are characterized by their high contents of sphingolipids, cholesterol and saturated fatty acids and a reduced content of polyunsaturated fatty acids (PUFA). Here, we have purified lipid rafts of human frontal brain cortex from normal and Alzheimer's disease (AD) and characterized their biochemical lipid composition. The results revealed that lipid rafts from AD brains exhibit aberrant lipid profiles compared to healthy brains. In particular, lipid rafts from AD brains displayed abnormally low levels of n-3 long chain polyunsaturated fatty acids (LCPUFA, mainly 22:6n-3, docosahexaenoic acid) and monoenes (mainly 18:1n-9, oleic acid), as well as reduced unsaturation and peroxidability indexes. Also, multiple relationships between phospholipids and fatty acids were altered in AD lipid rafts. Importantly, no changes were observed in the mole percentage of lipid classes and fatty acids in rafts from normal brains throughout the lifespan (24-85 years). These indications point to the existence of homeostatic mechanisms preserving lipid raft status in normal frontal cortex. The disruption of such mechanisms in AD brains leads to a considerable increase in lipid raft order and viscosity, which may explain the alterations in lipid raft signaling observed in AD. IOS Press https://hdl.handle.net/2445/154913 |
| spellingShingle | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex Martín, Virginia Lípids Escorça cerebral Malaltia d'Alzheimer Lipids Cerebral cortex Alzheimer's disease |
| status_str | publishedVersion |
| title | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex |
| title_full | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex |
| title_fullStr | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex |
| title_full_unstemmed | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex |
| title_short | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex |
| title_sort | Lipid alterations in lipid rafts from alzheimer's disease human brain cortex |
| topic | Lípids Escorça cerebral Malaltia d'Alzheimer Lipids Cerebral cortex Alzheimer's disease |
| topic_facet | Lípids Escorça cerebral Malaltia d'Alzheimer Lipids Cerebral cortex Alzheimer's disease |
| url | https://hdl.handle.net/2445/154913 |