Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer
Background/Objectives: Lymph node metastases (LNM) undetected by standard hematoxylin and eosin (H&E) have been associated with unfavorable prognosis in colorectal cancer. The One-Step Nucleic Acid Amplification (OSNA) assay has demonstrated superior sensitivity in detecting LNM compared to H&E. We...
| Autores: | ; ; ; ; ; ; ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:ddd.uab.cat:318774 |
| Acceso en línea: | https://ddd.uab.cat/record/318774 https://dx.doi.org/urn:doi:10.3390/cancers17132141 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Rectal carcinoma Lymph node metastases Staging Diagnosis Prognosis OSNA |
| _version_ | 1878739989050687488 |
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| author | Liu, Qing|||0000-0003-1784-6400 |
| author2 | López-Prades, Sandra|||0000-0001-8208-2959 Saez de Gordoa, Karmele|||0000-0001-8106-6121 Rodrigo Calvo, María Teresa|||0000-0001-6675-3425 Garcia Gerona, Mireia|||0000-0003-3445-9744 Ruiz Martin, Juan Romo-Navarro, Ángel|||0000-0002-7094-8484 Pinilla Pagnon, Ignacio|||0000-0002-6120-8302 Tarragona, Jordi|||0000-0002-1459-4087 Otero Alén, Begoña|||0000-0002-2876-6732 Camps Polo, Jordi|||0000-0003-2929-4228 Archilla, Ivan|||0000-0002-2142-0608 Cuatrecasas, Miriam|||0000-0003-3063-0110 |
| author2_role | author author author author author author author author author author author author |
| author_browse | Archilla, Ivan|||0000-0002-2142-0608 Camps Polo, Jordi|||0000-0003-2929-4228 Cuatrecasas, Miriam|||0000-0003-3063-0110 Garcia Gerona, Mireia|||0000-0003-3445-9744 Liu, Qing|||0000-0003-1784-6400 López-Prades, Sandra|||0000-0001-8208-2959 Otero Alén, Begoña|||0000-0002-2876-6732 Pinilla Pagnon, Ignacio|||0000-0002-6120-8302 Rodrigo Calvo, María Teresa|||0000-0001-6675-3425 Romo-Navarro, Ángel|||0000-0002-7094-8484 Ruiz Martin, Juan Saez de Gordoa, Karmele|||0000-0001-8106-6121 Tarragona, Jordi|||0000-0002-1459-4087 |
| author_facet | Liu, Qing|||0000-0003-1784-6400 López-Prades, Sandra|||0000-0001-8208-2959 Saez de Gordoa, Karmele|||0000-0001-8106-6121 Rodrigo Calvo, María Teresa|||0000-0001-6675-3425 Garcia Gerona, Mireia|||0000-0003-3445-9744 Ruiz Martin, Juan Romo-Navarro, Ángel|||0000-0002-7094-8484 Pinilla Pagnon, Ignacio|||0000-0002-6120-8302 Tarragona, Jordi|||0000-0002-1459-4087 Otero Alén, Begoña|||0000-0002-2876-6732 Camps Polo, Jordi|||0000-0003-2929-4228 Archilla, Ivan|||0000-0002-2142-0608 Cuatrecasas, Miriam|||0000-0003-3063-0110 |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Background/Objectives: Lymph node metastases (LNM) undetected by standard hematoxylin and eosin (H&E) have been associated with unfavorable prognosis in colorectal cancer. The One-Step Nucleic Acid Amplification (OSNA) assay has demonstrated superior sensitivity in detecting LNM compared to H&E. We aimed to assess the performance of OSNA in detecting LNM, as well as its prognostic value in rectal cancer (RC) patients. Methods: Lymph nodes (LNs) of patients from 15 centers were analyzed by both H&E and OSNA. The total tumor load (TTL) was defined as the sum of cytokeratin 19 mRNA copies/µL in all LNs from a surgical specimen, using a threshold of 250 copies/μL for OSNA positivity. Cox proportional hazard regression was used to assess the effect of TTL ≥ 250 or 6000 copies/μL on cancer-specific survival (CSS) and recurrence-free survival (RFS), with Firth's method applied to account for low event rate. Results: A total of 97 RC patients were included. Of these, 84 patients were eligible for survival analysis. The sensitivity and specificity of OSNA, compared to H&E, were 91.7% and 84.7%, respectively. TTL ≥ 6000 versus <6000 copies/μL was related to worse CSS and RFS. When dividing TTL into three groups: ≤250, 250-6000, and. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_cbb1d2e54d3945a3a0c4b686accada1f |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:318774 |
| publishDate | 2025 |
| publishDateSort | 2025 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer A Multicenter Study Liu, Qing|||0000-0003-1784-6400 López-Prades, Sandra|||0000-0001-8208-2959 Saez de Gordoa, Karmele|||0000-0001-8106-6121 Rodrigo Calvo, María Teresa|||0000-0001-6675-3425 Garcia Gerona, Mireia|||0000-0003-3445-9744 Ruiz Martin, Juan Romo-Navarro, Ángel|||0000-0002-7094-8484 Pinilla Pagnon, Ignacio|||0000-0002-6120-8302 Tarragona, Jordi|||0000-0002-1459-4087 Otero Alén, Begoña|||0000-0002-2876-6732 Camps Polo, Jordi|||0000-0003-2929-4228 Archilla, Ivan|||0000-0002-2142-0608 Cuatrecasas, Miriam|||0000-0003-3063-0110 Rectal carcinoma Lymph node metastases Staging Diagnosis Prognosis OSNA Background/Objectives: Lymph node metastases (LNM) undetected by standard hematoxylin and eosin (H&E) have been associated with unfavorable prognosis in colorectal cancer. The One-Step Nucleic Acid Amplification (OSNA) assay has demonstrated superior sensitivity in detecting LNM compared to H&E. We aimed to assess the performance of OSNA in detecting LNM, as well as its prognostic value in rectal cancer (RC) patients. Methods: Lymph nodes (LNs) of patients from 15 centers were analyzed by both H&E and OSNA. The total tumor load (TTL) was defined as the sum of cytokeratin 19 mRNA copies/µL in all LNs from a surgical specimen, using a threshold of 250 copies/μL for OSNA positivity. Cox proportional hazard regression was used to assess the effect of TTL ≥ 250 or 6000 copies/μL on cancer-specific survival (CSS) and recurrence-free survival (RFS), with Firth's method applied to account for low event rate. Results: A total of 97 RC patients were included. Of these, 84 patients were eligible for survival analysis. The sensitivity and specificity of OSNA, compared to H&E, were 91.7% and 84.7%, respectively. TTL ≥ 6000 versus <6000 copies/μL was related to worse CSS and RFS. When dividing TTL into three groups: ≤250, 250-6000, and. https://ddd.uab.cat/record/318774 https://dx.doi.org/urn:doi:10.3390/cancers17132141 |
| spellingShingle | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer Liu, Qing|||0000-0003-1784-6400 Rectal carcinoma Lymph node metastases Staging Diagnosis Prognosis OSNA |
| title | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer |
| title_full | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer |
| title_fullStr | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer |
| title_full_unstemmed | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer |
| title_short | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer |
| title_sort | Performance and Prognostic Relevance of Lymph Node Assessment by One-Step Nucleic Acid Amplification Assay in Rectal Cancer |
| topic | Rectal carcinoma Lymph node metastases Staging Diagnosis Prognosis OSNA |
| topic_facet | Rectal carcinoma Lymph node metastases Staging Diagnosis Prognosis OSNA |
| url | https://ddd.uab.cat/record/318774 https://dx.doi.org/urn:doi:10.3390/cancers17132141 |