Electrochemical aptasensor for thrombin based on polyfunctionalized dendritic nanodevice as natural enzyme-free labeling element
A novel dendritic hybrid nanomaterial with biorecognition and catalytic properties was synthesized by covalent attachment of specific aptamers on the surface of poly(amidoamine) G-4.5 dendrimers and further in situ growth of Pt nanoparticles encapsulated into the polymeric cavities. This nanodevice...
| Authors: | ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2025 |
| Country: | España |
| Institution: | Universidad Complutense de Madrid (UCM) |
| Repository: | Docta Complutense |
| Language: | English |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/129439 |
| Online Access: | https://hdl.handle.net/20.500.14352/129439 |
| Access Level: | Open access |
| Keyword: | 54 Electrochemical biosensor Dendrimer Thrombin Aptamer Natural enzyme-free detection Platinum nanoparticles Química 2301 Química Analítica |
| Summary: | A novel dendritic hybrid nanomaterial with biorecognition and catalytic properties was synthesized by covalent attachment of specific aptamers on the surface of poly(amidoamine) G-4.5 dendrimers and further in situ growth of Pt nanoparticles encapsulated into the polymeric cavities. This nanodevice was employed as natural enzyme-free labeling element to design an amperometric aptasensor with sandwich-type architecture for the specific detection of thrombin, by using screen-printed carbon electrodes functionalized with Au nanoparticles and capture aptamers as transduction interface. The aptasensor was employed to detect the cardiovascular biomarker in the broad range from 1.0 pM to 1.5 nM, with detection limit of 200 fM. This electroanalytical device also showed high specificity, reproducibility and stability, retaining 92 % of its initial biosensing capacity after 50 days of storage at 4ºC. The aptasensor was successfully employed to quantify thrombin in spiked human serum samples. |
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