Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers
The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass...
| Autores: | ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Estado: | Published version |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Pompeu Fabra |
| Repositorio: | Repositorio Digital de la UPF |
| OAI Identifier: | oai:repositori.upf.edu:10230/48495 |
| Acceso en línea: | http://hdl.handle.net/10230/48495 http://dx.doi.org/10.3390/ijms22084000 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Classic drugs of abuse Gas chromatography-mass spectrometry High-resolution mass spectrometry Liquid chromatography New psychoactive substances (NPS) Novel synthetic opioids (NSO) Urine |
| _version_ | 1878739274308780032 |
|---|---|
| author | Marchei, Emilia |
| author2 | Ferri, Maria Alias Torrens, Marta Farré Albaladejo, Magí Pacifici, Roberta Pichini, Simona Pellegrini, Manuela |
| author2_role | author author author author author author |
| author_browse | Farré Albaladejo, Magí Ferri, Maria Alias Marchei, Emilia Pacifici, Roberta Pellegrini, Manuela Pichini, Simona Torrens, Marta |
| author_facet | Marchei, Emilia Ferri, Maria Alias Torrens, Marta Farré Albaladejo, Magí Pacifici, Roberta Pichini, Simona Pellegrini, Manuela |
| author_role | author |
| collection | Repositorio Digital de la UPF |
| description | The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) were used for a screening analysis of classic drugs and new psychoactive substances and their metabolites in urine of formed heroin addicts under methadone maintenance therapy. Sample preparation involved a liquid-liquid extraction. The UHPLC-HRMS method included Accucore™ phenyl Hexyl (100 × 2.1 mm, 2.6 μm, Thermo, USA) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2 mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2 mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode for substances identification (mass range 100-1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m, 250 µm i.d, film thickness 0.25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40-550 m/z). Urine samples from 296 patients with a history of opioid use disorder were examined. Around 80 different psychoactive substances and/or metabolites were identified, being methadone and metabolites the most prevalent ones. The possibility to screen for a huge number of psychotropic substances can be useful in suspected drug related fatalities or acute intoxication/exposure occurring in emergency departments and drug addiction services. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_be5a8860f7e2c01fe897af51e79314f0 |
| institution | Universitat Pompeu Fabra |
| instname_str | Universitat Pompeu Fabra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositori.upf.edu:10230/48495 |
| publishDate | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Repositorio Digital de la UPF |
| spelling | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers Marchei, Emilia Ferri, Maria Alias Torrens, Marta Farré Albaladejo, Magí Pacifici, Roberta Pichini, Simona Pellegrini, Manuela Classic drugs of abuse Gas chromatography-mass spectrometry High-resolution mass spectrometry Liquid chromatography New psychoactive substances (NPS) Novel synthetic opioids (NSO) Urine The use of the new psychoactive substances is continuously growing and the implementation of accurate and sensible analysis in biological matrices of users is relevant and fundamental for clinical and forensic purposes. Two different analytical technologies, high-sensitivity gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) were used for a screening analysis of classic drugs and new psychoactive substances and their metabolites in urine of formed heroin addicts under methadone maintenance therapy. Sample preparation involved a liquid-liquid extraction. The UHPLC-HRMS method included Accucore™ phenyl Hexyl (100 × 2.1 mm, 2.6 μm, Thermo, USA) column with a gradient mobile phase consisting of mobile phase A (ammonium formate 2 mM in water, 0.1% formic acid) and mobile phase B (ammonium formate 2 mM in methanol/acetonitrile 50:50 (v/v), 0.1% formic acid) and a full-scan data-dependent MS2 (ddMS2) mode for substances identification (mass range 100-1000 m/z). The GC-MS method employed an ultra-Inert Intuvo GC column (HP-5MS UI, 30 m, 250 µm i.d, film thickness 0.25 µm; Agilent Technologies, Santa Clara, CA, USA) and electron-impact (EI) mass spectra were recorded in total ion monitoring mode (scan range 40-550 m/z). Urine samples from 296 patients with a history of opioid use disorder were examined. Around 80 different psychoactive substances and/or metabolites were identified, being methadone and metabolites the most prevalent ones. The possibility to screen for a huge number of psychotropic substances can be useful in suspected drug related fatalities or acute intoxication/exposure occurring in emergency departments and drug addiction services. MDPI http://hdl.handle.net/10230/48495 http://dx.doi.org/10.3390/ijms22084000 |
| spellingShingle | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers Marchei, Emilia Classic drugs of abuse Gas chromatography-mass spectrometry High-resolution mass spectrometry Liquid chromatography New psychoactive substances (NPS) Novel synthetic opioids (NSO) Urine |
| status_str | publishedVersion |
| title | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers |
| title_full | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers |
| title_fullStr | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers |
| title_full_unstemmed | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers |
| title_short | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers |
| title_sort | Ultra-high performance liquid chromatography-high resolution mass spectrometry and high-sensitivity gas chromatography-mass spectrometry screening of classic drugs and new psychoactive substances and metabolites in urine of consumers |
| topic | Classic drugs of abuse Gas chromatography-mass spectrometry High-resolution mass spectrometry Liquid chromatography New psychoactive substances (NPS) Novel synthetic opioids (NSO) Urine |
| topic_facet | Classic drugs of abuse Gas chromatography-mass spectrometry High-resolution mass spectrometry Liquid chromatography New psychoactive substances (NPS) Novel synthetic opioids (NSO) Urine |
| url | http://hdl.handle.net/10230/48495 http://dx.doi.org/10.3390/ijms22084000 |