Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction
Discarded red beetroot (DRB) is an organic waste generated in the food industry. This study is focused on the valorization of DRB through the recovery of bioactive compounds. The characterization of the DRB juice confirmed a higher content of antioxidants (DPPH 504 ± 24 µmol TE/L, FRAP 10920 ± 440 µ...
| Authors: | ; ; ; ; |
|---|---|
| Format: | Dataset |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Universidad de Valladolid |
| Repository: | UVaDOC. Repositorio Documental de la Universidad de Valladolid |
| OAI Identifier: | oai:uvadoc.uva.es:10324/70813 |
| Online Access: | https://doi.org/10.71569/bcmk-v984 https://uvadoc.uva.es/handle/10324/70813 |
| Access Level: | Open access |
| Keyword: | Microwave-assisted extraction, Galacturonic acid, Pectooligosaccharides, Polyethylene glycol, Antioxidants, Betalains |
| _version_ | 1878739247043706880 |
|---|---|
| author | Amo Mateos, Esther Del |
| author2 | Fernández Delgado, Marina Lucas Yagüe, Susana García Cubero, María Teresa Coca Sanz, Mónica |
| author2_role | author author author author |
| author_browse | Amo Mateos, Esther Del Coca Sanz, Mónica Fernández Delgado, Marina García Cubero, María Teresa Lucas Yagüe, Susana |
| author_facet | Amo Mateos, Esther Del Fernández Delgado, Marina Lucas Yagüe, Susana García Cubero, María Teresa Coca Sanz, Mónica |
| author_role | author |
| collection | UVaDOC. Repositorio Documental de la Universidad de Valladolid |
| description | Discarded red beetroot (DRB) is an organic waste generated in the food industry. This study is focused on the valorization of DRB through the recovery of bioactive compounds. The characterization of the DRB juice confirmed a higher content of antioxidants (DPPH 504 ± 24 µmol TE/L, FRAP 10920 ± 440 µmol TE/L, ABTS 22012 ± 592 µmol TE/L), phenolic compounds (1789 ± 56 mg GAE/L), flavonoids (471± 17 mg CE/L) and betalains (1426 ± 24 mg/L) than commercial juices. DRB pomace contains pectin that was recovered by microwave extraction aided by a surfactant (polyethylene glycol, PEG4000). Conditions (temperature, time and surfactant concentration) for the extraction of galacturonic acid (GalA) or pectooligosaccharides (POS) were optimized by a central composite experimental design. POS were extracted at high temperatures (160 °C, 5.3 min, 8.4 g PEG4000/L, yield of 271.2 g POS/kg dry pomace). In comparison, galacturonic acid extraction was favored at moderate conditions (137 °C, 5 min, 2.5 g PEG4000/L, yield of 120.1 g GalA/kg dry pomace). The characterization of the freeze-dried hydrolysates revealed that the solid obtained under moderate temperature conditions (137 ºC) showed a higher GalA content (49.5%) and lower neutral sugars (11.4%), as GalA degrades at lower temperatures than pentoses. The recovered pectin can be considered high-methoxyl pectin, as the degree of esterification was higher than 50%. FTIR spectra of the freeze-dried hydrolysates showed functional groups consistent with pectin. MALDI-TOF-MS analysis revealed the presence of oligosaccharides of hexoses and pentoses with different structures and degrees of polymerization. Thus, DRB, a low-value vegetable waste, can be converted into high-value-added bioproducts in a biorefinery framework. |
| eu_rights_str_mv | openAccess |
| format | dataset |
| id | ES_bdd3eefbe10abc018f7bd00a90ee0d6c |
| institution | Universidad de Valladolid |
| instname_str | Universidad de Valladolid |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:uvadoc.uva.es:10324/70813 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| reponame_str | UVaDOC. Repositorio Documental de la Universidad de Valladolid |
| spelling | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction Amo Mateos, Esther Del Fernández Delgado, Marina Lucas Yagüe, Susana García Cubero, María Teresa Coca Sanz, Mónica Microwave-assisted extraction, Galacturonic acid, Pectooligosaccharides, Polyethylene glycol, Antioxidants, Betalains Discarded red beetroot (DRB) is an organic waste generated in the food industry. This study is focused on the valorization of DRB through the recovery of bioactive compounds. The characterization of the DRB juice confirmed a higher content of antioxidants (DPPH 504 ± 24 µmol TE/L, FRAP 10920 ± 440 µmol TE/L, ABTS 22012 ± 592 µmol TE/L), phenolic compounds (1789 ± 56 mg GAE/L), flavonoids (471± 17 mg CE/L) and betalains (1426 ± 24 mg/L) than commercial juices. DRB pomace contains pectin that was recovered by microwave extraction aided by a surfactant (polyethylene glycol, PEG4000). Conditions (temperature, time and surfactant concentration) for the extraction of galacturonic acid (GalA) or pectooligosaccharides (POS) were optimized by a central composite experimental design. POS were extracted at high temperatures (160 °C, 5.3 min, 8.4 g PEG4000/L, yield of 271.2 g POS/kg dry pomace). In comparison, galacturonic acid extraction was favored at moderate conditions (137 °C, 5 min, 2.5 g PEG4000/L, yield of 120.1 g GalA/kg dry pomace). The characterization of the freeze-dried hydrolysates revealed that the solid obtained under moderate temperature conditions (137 ºC) showed a higher GalA content (49.5%) and lower neutral sugars (11.4%), as GalA degrades at lower temperatures than pentoses. The recovered pectin can be considered high-methoxyl pectin, as the degree of esterification was higher than 50%. FTIR spectra of the freeze-dried hydrolysates showed functional groups consistent with pectin. MALDI-TOF-MS analysis revealed the presence of oligosaccharides of hexoses and pentoses with different structures and degrees of polymerization. Thus, DRB, a low-value vegetable waste, can be converted into high-value-added bioproducts in a biorefinery framework. Instituto de Procesos Sostenibles Spanish Ministry of Science, Innovation and Universities (PID2020-115110RB-I00/AEI/10.13039/501100011033) https://doi.org/10.71569/bcmk-v984 https://uvadoc.uva.es/handle/10324/70813 |
| spellingShingle | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction Amo Mateos, Esther Del Microwave-assisted extraction, Galacturonic acid, Pectooligosaccharides, Polyethylene glycol, Antioxidants, Betalains |
| status_str | publishedVersion |
| title | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction |
| title_full | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction |
| title_fullStr | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction |
| title_full_unstemmed | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction |
| title_short | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction |
| title_sort | Valorization of discarded red beetroot through the recovery of bioactive compounds and the production of pectin by surfactant-assisted microwave extraction |
| topic | Microwave-assisted extraction, Galacturonic acid, Pectooligosaccharides, Polyethylene glycol, Antioxidants, Betalains |
| topic_facet | Microwave-assisted extraction, Galacturonic acid, Pectooligosaccharides, Polyethylene glycol, Antioxidants, Betalains |
| url | https://doi.org/10.71569/bcmk-v984 https://uvadoc.uva.es/handle/10324/70813 |