Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain
[EN]Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulati...
| Authors: | ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Universidad de Salamanca (USAL) |
| Repository: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/145538 |
| Online Access: | http://hdl.handle.net/10366/145538 |
| Access Level: | Open access |
| Keyword: | Pea Pisum sativum Rhizobium laguerreae Symbiosis Quorum sensing Nodulation effectiveness Nitrogen fixation 2414 Microbiología |
| _version_ | 1878739233847377920 |
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| author | Flores Félix, José David |
| author2 | Carro García, Lorena Cerda Castillo, María Eugenia Squartini, Andrea Rivas González, Raúl Velázquez Pérez, María Encarnación |
| author2_role | author author author author author |
| author_browse | Carro García, Lorena Cerda Castillo, María Eugenia Flores Félix, José David Rivas González, Raúl Squartini, Andrea Velázquez Pérez, María Encarnación |
| author_facet | Flores Félix, José David Carro García, Lorena Cerda Castillo, María Eugenia Squartini, Andrea Rivas González, Raúl Velázquez Pérez, María Encarnación |
| author_role | author |
| collection | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| description | [EN]Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. laguerreae sv. viciae. Representative strains of these clusters were able to produce cellulose and cellulases, which are two key molecules in the legume infection process. They formed biofilms and produced acyl-homoserine lactones (AHLs), which are involved in the quorum sensing regulation process. They also exhibited several plant growth promotion mechanisms, including phosphate solubilization, siderophore, and indole acetic acid production and symbiotic atmospheric nitrogen fixation. All strains showed high symbiotic efficiency on pea plants, indicating that strains of R. laguerreae sv. viciae are promising candidates for the biofertilization of this legume worldwide. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_bd9b14cef46dfd35df7e404c552ce29d |
| institution | Universidad de Salamanca (USAL) |
| instname_str | Universidad de Salamanca (USAL) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:gredos.usal.es:10366/145538 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| spelling | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain Flores Félix, José David Carro García, Lorena Cerda Castillo, María Eugenia Squartini, Andrea Rivas González, Raúl Velázquez Pérez, María Encarnación Pea Pisum sativum Rhizobium laguerreae Symbiosis Quorum sensing Nodulation effectiveness Nitrogen fixation 2414 Microbiología [EN]Pisum sativum L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries. In this work, we analyzed the rhizobial strains nodulating the pea in a region from Northwestern Spain, where this legume is widely cultivated. The isolated strains were genetically diverse, and the phylogenetic analysis of core and symbiotic genes showed that these strains belong to different clusters related to R. laguerreae sv. viciae. Representative strains of these clusters were able to produce cellulose and cellulases, which are two key molecules in the legume infection process. They formed biofilms and produced acyl-homoserine lactones (AHLs), which are involved in the quorum sensing regulation process. They also exhibited several plant growth promotion mechanisms, including phosphate solubilization, siderophore, and indole acetic acid production and symbiotic atmospheric nitrogen fixation. All strains showed high symbiotic efficiency on pea plants, indicating that strains of R. laguerreae sv. viciae are promising candidates for the biofertilization of this legume worldwide. MDPI http://hdl.handle.net/10366/145538 |
| spellingShingle | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain Flores Félix, José David Pea Pisum sativum Rhizobium laguerreae Symbiosis Quorum sensing Nodulation effectiveness Nitrogen fixation 2414 Microbiología |
| status_str | publishedVersion |
| title | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain |
| title_full | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain |
| title_fullStr | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain |
| title_full_unstemmed | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain |
| title_short | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain |
| title_sort | Analysis of the Interaction between Pisum sativum L. and Rhizobium laguerreae Strains Nodulating This Legume in Northwest Spain |
| topic | Pea Pisum sativum Rhizobium laguerreae Symbiosis Quorum sensing Nodulation effectiveness Nitrogen fixation 2414 Microbiología |
| topic_facet | Pea Pisum sativum Rhizobium laguerreae Symbiosis Quorum sensing Nodulation effectiveness Nitrogen fixation 2414 Microbiología |
| url | http://hdl.handle.net/10366/145538 |