Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinf...
| Authors: | ; ; ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Universidad Complutense de Madrid (UCM) |
| Repository: | Docta Complutense |
| Language: | English |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/104025 |
| Online Access: | https://hdl.handle.net/20.500.14352/104025 |
| Access Level: | Open access |
| Keyword: | 615 Cytotoxicity EA.hy926 Ipconazole fungicide Oxidative stress Proinflammation Farmacología (Farmacia) 3209 Farmacología |
| _version_ | 1878739072814415872 |
|---|---|
| author | Ruiz Yance, Iris |
| author2 | Siguas, Junior Bardales, Brandy Robles Castañeda, Ingrid Cordova, Karen Ypushima, Alina Estela Villar, Esteban Quintana Criollo, Carlos Estacio, Darwin Rodríguez, José Luis |
| author2_role | author author author author author author author author author |
| author_browse | Bardales, Brandy Cordova, Karen Estacio, Darwin Estela Villar, Esteban Quintana Criollo, Carlos Robles Castañeda, Ingrid Rodríguez, José Luis Ruiz Yance, Iris Siguas, Junior Ypushima, Alina |
| author_facet | Ruiz Yance, Iris Siguas, Junior Bardales, Brandy Robles Castañeda, Ingrid Cordova, Karen Ypushima, Alina Estela Villar, Esteban Quintana Criollo, Carlos Estacio, Darwin Rodríguez, José Luis |
| author_role | author |
| collection | Docta Complutense |
| description | Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinflammation of EA.hy926 endothelial cells in response to ipconazole exposure. Using the MTT assay, ipconazole was found to produce a dose-dependent reduction (*** p < 0.001; concentrations of 20, 50 and 100 µM) of cell viability in EA.hy926 with an IC50 of 29 µM. Also, ipconazole induced a significant increase in ROS generation (** p < 0.01), caspase 3/7 (** p < 0.01), cell death (BAX, APAF1, BNIP3, CASP3 and AKT1) and proinflammatory (NLRP3, CASP1, IL1β, NFκB, IL6 and TNFα) biomarkers, as well as a reduction in antioxidant (NRF2 and GPx) biomarkers. These results demonstrated that oxidative stress, proinflammatory activity and cell death could be responsible for the cytotoxic effect produced by the fungicide ipconazole, such that this triazole compound should be considered as a possible risk factor in the development of alterations in cellular homeostasis. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_ba97b67e8b7f8aa4d286e171954e00a8 |
| institution | Universidad Complutense de Madrid (UCM) |
| instname_str | Universidad Complutense de Madrid (UCM) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:docta.ucm.es:20.500.14352/104025 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Docta Complutense |
| spelling | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells Ruiz Yance, Iris Siguas, Junior Bardales, Brandy Robles Castañeda, Ingrid Cordova, Karen Ypushima, Alina Estela Villar, Esteban Quintana Criollo, Carlos Estacio, Darwin Rodríguez, José Luis 615 Cytotoxicity EA.hy926 Ipconazole fungicide Oxidative stress Proinflammation Farmacología (Farmacia) 3209 Farmacología Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinflammation of EA.hy926 endothelial cells in response to ipconazole exposure. Using the MTT assay, ipconazole was found to produce a dose-dependent reduction (*** p < 0.001; concentrations of 20, 50 and 100 µM) of cell viability in EA.hy926 with an IC50 of 29 µM. Also, ipconazole induced a significant increase in ROS generation (** p < 0.01), caspase 3/7 (** p < 0.01), cell death (BAX, APAF1, BNIP3, CASP3 and AKT1) and proinflammatory (NLRP3, CASP1, IL1β, NFκB, IL6 and TNFα) biomarkers, as well as a reduction in antioxidant (NRF2 and GPx) biomarkers. These results demonstrated that oxidative stress, proinflammatory activity and cell death could be responsible for the cytotoxic effect produced by the fungicide ipconazole, such that this triazole compound should be considered as a possible risk factor in the development of alterations in cellular homeostasis. MDPI https://hdl.handle.net/20.500.14352/104025 |
| spellingShingle | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells Ruiz Yance, Iris 615 Cytotoxicity EA.hy926 Ipconazole fungicide Oxidative stress Proinflammation Farmacología (Farmacia) 3209 Farmacología |
| title | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells |
| title_full | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells |
| title_fullStr | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells |
| title_full_unstemmed | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells |
| title_short | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells |
| title_sort | Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells |
| topic | 615 Cytotoxicity EA.hy926 Ipconazole fungicide Oxidative stress Proinflammation Farmacología (Farmacia) 3209 Farmacología |
| topic_facet | 615 Cytotoxicity EA.hy926 Ipconazole fungicide Oxidative stress Proinflammation Farmacología (Farmacia) 3209 Farmacología |
| url | https://hdl.handle.net/20.500.14352/104025 |