The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats
Although the pharmacological and behavioural interactions between cocaine and alcohol are well established, less is known about how polyconsumption of these drugs affects the neurotransmitter systems involved in their psychoactive effects and in particular, in the process of addiction. Here, rats of...
| Authors: | ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2022 |
| Country: | España |
| Institution: | Universidad de Castilla-La Mancha |
| Repository: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:dnet:ruidera_____::f089a8b3438338aee356c2205ab89623 |
| Online Access: | https://doi.org/10.1016/j.neuropharm.2021.108937 https://www.sciencedirect.com/science/article/pii/S0028390821004949?__cf_chl_tk=oU8R5D4c_eh2weLl4v3FPBWII.GJT0HqtAO2kesPg0s-1777977691-1.0.1.1-YvP80SXtqsnELp0J6wt4BWNjczZR3mdrymrHSBxKwqI https://hdl.handle.net/10578/48324 |
| Access Level: | Open access |
| Keyword: | Co-abuse Cocaine Ethanol GABAergic Glutamatergic and endocannabinoid systems Mesocorticolimbic system Rat |
| _version_ | 1878738995516538880 |
|---|---|
| author | Marcos, Alberto |
| author2 | Ballesteros Yáñez, Inmaculada Castillo Sarmiento, Carlos Alberto Pardo, Felipe Roura Martínez, David Muñoz Rodríguez, José Ramón Higueras Matas, Alejandro Ambrosio, Emilio |
| author2_role | author author author author author author author |
| author_browse | Ambrosio, Emilio Ballesteros Yáñez, Inmaculada Castillo Sarmiento, Carlos Alberto Higueras Matas, Alejandro Marcos, Alberto Muñoz Rodríguez, José Ramón Pardo, Felipe Roura Martínez, David |
| author_facet | Marcos, Alberto Ballesteros Yáñez, Inmaculada Castillo Sarmiento, Carlos Alberto Pardo, Felipe Roura Martínez, David Muñoz Rodríguez, José Ramón Higueras Matas, Alejandro Ambrosio, Emilio |
| author_role | author |
| collection | RUIdeRA. Repositorio Institucional de la UCLM |
| description | Although the pharmacological and behavioural interactions between cocaine and alcohol are well established, less is known about how polyconsumption of these drugs affects the neurotransmitter systems involved in their psychoactive effects and in particular, in the process of addiction. Here, rats of both sexes at two stages of development were studied under a chronic regime of intravenous cocaine and/or alcohol administration. Brain samples from the medial prefrontal cortex, nucleus accumbens, hippocampus and amygdala were extracted to analyse the mRNA expression of genes encoding subunits of the GABA, NMDA and AMPA receptors, as well as the expression of the CB1 receptor, and that of enzymes related to the biosynthesis and degradation of endocannabinoids. Moreover, two synaptic scaffold proteins related to GABA and NMDA receptors, gephyrin and PSD-95, were quantified in Western blots. Significant interactions between cocaine and alcohol were common, affecting the GABAergic and endocannabinoid systems in the medial prefrontal cortex and amygdala of young adults, whereas such interactions were evident in the glutamatergic and endocannabinoid systems in adults, as well as a more pronounced sex effect. Significant interactions between these drugs affecting the scaffold proteins were evident in the medial prefrontal cortex and nucleus accumbens of young adults, and in the nucleus accumbens and amygdala of adults, but not in the hippocampus. These results highlight the importance of considering the interactions between cocaine and alcohol on neurotransmitter systems in the context of polyconsumption, specifically when treating problems of abuse of these two substances. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b918e84d2c2e919b67148564eeb3d84e |
| institution | Universidad de Castilla-La Mancha |
| instname_str | Universidad de Castilla-La Mancha |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:dnet:ruidera_____::f089a8b3438338aee356c2205ab89623 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | RUIdeRA. Repositorio Institucional de la UCLM |
| spelling | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats Marcos, Alberto Ballesteros Yáñez, Inmaculada Castillo Sarmiento, Carlos Alberto Pardo, Felipe Roura Martínez, David Muñoz Rodríguez, José Ramón Higueras Matas, Alejandro Ambrosio, Emilio Co-abuse Cocaine Ethanol GABAergic Glutamatergic and endocannabinoid systems Mesocorticolimbic system Rat Although the pharmacological and behavioural interactions between cocaine and alcohol are well established, less is known about how polyconsumption of these drugs affects the neurotransmitter systems involved in their psychoactive effects and in particular, in the process of addiction. Here, rats of both sexes at two stages of development were studied under a chronic regime of intravenous cocaine and/or alcohol administration. Brain samples from the medial prefrontal cortex, nucleus accumbens, hippocampus and amygdala were extracted to analyse the mRNA expression of genes encoding subunits of the GABA, NMDA and AMPA receptors, as well as the expression of the CB1 receptor, and that of enzymes related to the biosynthesis and degradation of endocannabinoids. Moreover, two synaptic scaffold proteins related to GABA and NMDA receptors, gephyrin and PSD-95, were quantified in Western blots. Significant interactions between cocaine and alcohol were common, affecting the GABAergic and endocannabinoid systems in the medial prefrontal cortex and amygdala of young adults, whereas such interactions were evident in the glutamatergic and endocannabinoid systems in adults, as well as a more pronounced sex effect. Significant interactions between these drugs affecting the scaffold proteins were evident in the medial prefrontal cortex and nucleus accumbens of young adults, and in the nucleus accumbens and amygdala of adults, but not in the hippocampus. These results highlight the importance of considering the interactions between cocaine and alcohol on neurotransmitter systems in the context of polyconsumption, specifically when treating problems of abuse of these two substances. Elsevier https://doi.org/10.1016/j.neuropharm.2021.108937 https://www.sciencedirect.com/science/article/pii/S0028390821004949?__cf_chl_tk=oU8R5D4c_eh2weLl4v3FPBWII.GJT0HqtAO2kesPg0s-1777977691-1.0.1.1-YvP80SXtqsnELp0J6wt4BWNjczZR3mdrymrHSBxKwqI https://hdl.handle.net/10578/48324 |
| spellingShingle | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats Marcos, Alberto Co-abuse Cocaine Ethanol GABAergic Glutamatergic and endocannabinoid systems Mesocorticolimbic system Rat |
| title | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats |
| title_full | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats |
| title_fullStr | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats |
| title_full_unstemmed | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats |
| title_short | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats |
| title_sort | The interactions of alcohol and cocaine regulate the expression of genes involved in the GABAergic, glutamatergic and endocannabinoid systems of male and female rats |
| topic | Co-abuse Cocaine Ethanol GABAergic Glutamatergic and endocannabinoid systems Mesocorticolimbic system Rat |
| topic_facet | Co-abuse Cocaine Ethanol GABAergic Glutamatergic and endocannabinoid systems Mesocorticolimbic system Rat |
| url | https://doi.org/10.1016/j.neuropharm.2021.108937 https://www.sciencedirect.com/science/article/pii/S0028390821004949?__cf_chl_tk=oU8R5D4c_eh2weLl4v3FPBWII.GJT0HqtAO2kesPg0s-1777977691-1.0.1.1-YvP80SXtqsnELp0J6wt4BWNjczZR3mdrymrHSBxKwqI https://hdl.handle.net/10578/48324 |