Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9
The high-affinity K+ transporter HAK5 is the major contributor to root K+ uptake from dilute solutions in K+-starved Arabidopsis plants. Its functionality is tightly regulated and its activity is enhanced under K+ starvation by the transcriptional induction of the AtHAK5 gene, and by the activation...
| Authors: | ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2020 |
| Country: | España |
| Institution: | Universidad Miguel Hernández de Elche |
| Repository: | REDIUMH. Depósito Digital de la UMH |
| OAI Identifier: | oai:dspace.umh.es:11000/32436 |
| Online Access: | https://hdl.handle.net/11000/32436 |
| Access Level: | Open access |
| Keyword: | Arabidopsis AtCBL AtCIPK AtHAK5 potassium uptake CDU::5 - Ciencias puras y naturales::57 - Biología |
| _version_ | 1878738973051846657 |
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| author | Lara, Alberto |
| author2 | Ródenas, Reyes Andrés, Zaida Martínez, Vicente Quintero, Francisco J. Nieves-Cordones, Manuel Botella, M. Ángeles Rubio, Francisco |
| author2_role | author author author author author author author |
| author_browse | Andrés, Zaida Botella, M. Ángeles Lara, Alberto Martínez, Vicente Nieves-Cordones, Manuel Quintero, Francisco J. Rubio, Francisco Ródenas, Reyes |
| author_facet | Lara, Alberto Ródenas, Reyes Andrés, Zaida Martínez, Vicente Quintero, Francisco J. Nieves-Cordones, Manuel Botella, M. Ángeles Rubio, Francisco |
| author_role | author |
| collection | REDIUMH. Depósito Digital de la UMH |
| description | The high-affinity K+ transporter HAK5 is the major contributor to root K+ uptake from dilute solutions in K+-starved Arabidopsis plants. Its functionality is tightly regulated and its activity is enhanced under K+ starvation by the transcriptional induction of the AtHAK5 gene, and by the activation of the transporter via the AtCBL1–AtCIPK23 complex. In the present study, the 26 members of the Arabidopsis CIPK protein kinase family were screened in yeast for their capacity to activate HAK5-mediated K+ uptake. Among them, AtCIPK1 was the most efficient activator of AtHAK5. In addition, AtCIPK9, previously reported to participate in K+ homeostasis, also activated the transporter. In roots, the genes encoding AtCIPK1 and AtCIPK9 were induced by K+ deprivation and atcipk1 and atcipk9 Arabidopsis KO mutants showed a reduced AtHAK5-mediated Rb+ uptake. Activation of AtHAK5 by AtCIPK1 did not occur under hyperosmotic stress conditions, where AtCIPK1 function has been shown to be required to maintain plant growth. Taken together, our data contribute to the identification of the complex regulatory networks that control the highaffinity K+ transporter AtHAK5 and root K+ uptake. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b8a9746285e2c7556e7e793b60cc49d8 |
| institution | Universidad Miguel Hernández de Elche |
| instname_str | Universidad Miguel Hernández de Elche |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:dspace.umh.es:11000/32436 |
| publishDate | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| reponame_str | REDIUMH. Depósito Digital de la UMH |
| spelling | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 Lara, Alberto Ródenas, Reyes Andrés, Zaida Martínez, Vicente Quintero, Francisco J. Nieves-Cordones, Manuel Botella, M. Ángeles Rubio, Francisco Arabidopsis AtCBL AtCIPK AtHAK5 potassium uptake CDU::5 - Ciencias puras y naturales::57 - Biología The high-affinity K+ transporter HAK5 is the major contributor to root K+ uptake from dilute solutions in K+-starved Arabidopsis plants. Its functionality is tightly regulated and its activity is enhanced under K+ starvation by the transcriptional induction of the AtHAK5 gene, and by the activation of the transporter via the AtCBL1–AtCIPK23 complex. In the present study, the 26 members of the Arabidopsis CIPK protein kinase family were screened in yeast for their capacity to activate HAK5-mediated K+ uptake. Among them, AtCIPK1 was the most efficient activator of AtHAK5. In addition, AtCIPK9, previously reported to participate in K+ homeostasis, also activated the transporter. In roots, the genes encoding AtCIPK1 and AtCIPK9 were induced by K+ deprivation and atcipk1 and atcipk9 Arabidopsis KO mutants showed a reduced AtHAK5-mediated Rb+ uptake. Activation of AtHAK5 by AtCIPK1 did not occur under hyperosmotic stress conditions, where AtCIPK1 function has been shown to be required to maintain plant growth. Taken together, our data contribute to the identification of the complex regulatory networks that control the highaffinity K+ transporter AtHAK5 and root K+ uptake. Oxford University Press https://hdl.handle.net/11000/32436 |
| spellingShingle | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 Lara, Alberto Arabidopsis AtCBL AtCIPK AtHAK5 potassium uptake CDU::5 - Ciencias puras y naturales::57 - Biología |
| title | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 |
| title_full | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 |
| title_fullStr | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 |
| title_full_unstemmed | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 |
| title_short | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 |
| title_sort | Arabidopsis K+ transporter HAK5-mediated high-affinity root K+ uptake is regulated by protein kinases CIPK1 and CIPK9 |
| topic | Arabidopsis AtCBL AtCIPK AtHAK5 potassium uptake CDU::5 - Ciencias puras y naturales::57 - Biología |
| topic_facet | Arabidopsis AtCBL AtCIPK AtHAK5 potassium uptake CDU::5 - Ciencias puras y naturales::57 - Biología |
| url | https://hdl.handle.net/11000/32436 |