Genes involved in the adhesion and invasion of Arcobacter butzleri
Arcobacter butzleri is a foodborne pathogen that mainly causes enteritis in humans, but the number of cases of bacteraemia has increased in recent years. However, there is still limited knowledge on the pathogenic mechanisms of this bacterium. To investigate how A. butzleri causes disease, single kn...
| Autores: | ; ; ; ; |
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| Tipo de documento: | Artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad del País Vasco |
| Repositorio: | Addi. Archivo Digital para la Docencia y la Investigación |
| OAI Identifier: | oai:addi.ehu.eus:10810/68632 |
| Acceso en línea: | http://hdl.handle.net/10810/68632 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Arcobacter butzleri adhesion invasion culture cells gene function mutagenesis |
| _version_ | 1878738906115997696 |
|---|---|
| author | Baztarrika Uria, Itsaso |
| author2 | Wösten, Marc Alonso Monsalve, Rodrigo Martínez Ballesteros, Ilargi Martínez Malax-Echevarría, Irati |
| author2_role | author author author author |
| author_browse | Alonso Monsalve, Rodrigo Baztarrika Uria, Itsaso Martínez Ballesteros, Ilargi Martínez Malax-Echevarría, Irati Wösten, Marc |
| author_facet | Baztarrika Uria, Itsaso Wösten, Marc Alonso Monsalve, Rodrigo Martínez Ballesteros, Ilargi Martínez Malax-Echevarría, Irati |
| author_role | author |
| collection | Addi. Archivo Digital para la Docencia y la Investigación |
| description | Arcobacter butzleri is a foodborne pathogen that mainly causes enteritis in humans, but the number of cases of bacteraemia has increased in recent years. However, there is still limited knowledge on the pathogenic mechanisms of this bacterium. To investigate how A. butzleri causes disease, single knockout mutants were constructed in the cadF, ABU_RS00335, ciaB, and flaAB genes, which might be involved in adhesion and invasion properties. These mutants and the isogenic wild-type (WT) were then tested for their ability to adhere and invade human Caco-2 and HT29-MTX cells. The adhesion and invasion of A. butzleri RM4018 strain was also visualized by a Leica CTR 6500 confocal microscope. The adhesion and invasion abilities of mutants lacking the invasion antigen CiaB or a functional flagellum were lower than those of the WTs. However, the extent of the decrease varied depending on the strain and/or cell line. Mutants lacking the fibronectin (FN)-binding protein CadF consistently exhibited reduced abilities, while the inactivation of the other studied FN-binding protein, ABU_RS00335, led to a reduction in only one of the two strains tested. Therefore, the ciaB and flaAB genes appear to be important for A. butzleri adhesion and invasion properties, while cadF appears to be indispensable. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b753339bfe43da8b26128dacd08c9db7 |
| institution | Universidad del País Vasco |
| instname_str | Universidad del País Vasco |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:addi.ehu.eus:10810/68632 |
| publishDate | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| reponame_str | Addi. Archivo Digital para la Docencia y la Investigación |
| spelling | Genes involved in the adhesion and invasion of Arcobacter butzleri Baztarrika Uria, Itsaso Wösten, Marc Alonso Monsalve, Rodrigo Martínez Ballesteros, Ilargi Martínez Malax-Echevarría, Irati Arcobacter butzleri adhesion invasion culture cells gene function mutagenesis Arcobacter butzleri is a foodborne pathogen that mainly causes enteritis in humans, but the number of cases of bacteraemia has increased in recent years. However, there is still limited knowledge on the pathogenic mechanisms of this bacterium. To investigate how A. butzleri causes disease, single knockout mutants were constructed in the cadF, ABU_RS00335, ciaB, and flaAB genes, which might be involved in adhesion and invasion properties. These mutants and the isogenic wild-type (WT) were then tested for their ability to adhere and invade human Caco-2 and HT29-MTX cells. The adhesion and invasion of A. butzleri RM4018 strain was also visualized by a Leica CTR 6500 confocal microscope. The adhesion and invasion abilities of mutants lacking the invasion antigen CiaB or a functional flagellum were lower than those of the WTs. However, the extent of the decrease varied depending on the strain and/or cell line. Mutants lacking the fibronectin (FN)-binding protein CadF consistently exhibited reduced abilities, while the inactivation of the other studied FN-binding protein, ABU_RS00335, led to a reduction in only one of the two strains tested. Therefore, the ciaB and flaAB genes appear to be important for A. butzleri adhesion and invasion properties, while cadF appears to be indispensable. This work supported by the University of the Basque Country UPV/EHU [grant number GIU21/021]. I.B was awarded an Ikertalent Ph.D. fellowship by the Basque Government. Elsevier http://hdl.handle.net/10810/68632 |
| spellingShingle | Genes involved in the adhesion and invasion of Arcobacter butzleri Baztarrika Uria, Itsaso Arcobacter butzleri adhesion invasion culture cells gene function mutagenesis |
| title | Genes involved in the adhesion and invasion of Arcobacter butzleri |
| title_full | Genes involved in the adhesion and invasion of Arcobacter butzleri |
| title_fullStr | Genes involved in the adhesion and invasion of Arcobacter butzleri |
| title_full_unstemmed | Genes involved in the adhesion and invasion of Arcobacter butzleri |
| title_short | Genes involved in the adhesion and invasion of Arcobacter butzleri |
| title_sort | Genes involved in the adhesion and invasion of Arcobacter butzleri |
| topic | Arcobacter butzleri adhesion invasion culture cells gene function mutagenesis |
| topic_facet | Arcobacter butzleri adhesion invasion culture cells gene function mutagenesis |
| url | http://hdl.handle.net/10810/68632 |