Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses
Objective The aim of this study was to perform a comprehensive assessment of the placental aging process through senescence and apoptotic markers in late-onset small fetuses classified as SGA or FGR. Study Design A prospective nested case-control study in singleton pregnancies delivering at term inc...
| Authors: | ; ; ; ; ; ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Accepted version |
| Publication date: | 2018 |
| Country: | España |
| Institution: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repository: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/125602 |
| Online Access: | https://hdl.handle.net/2445/125602 |
| Access Level: | Open access |
| Keyword: | Creixement fetal Placenta Telòmer Fetal growth Telomere |
| _version_ | 1878738865539252224 |
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| author | Paules, Cristina |
| author2 | Dantas, Ana Paula Miranda, Jezid Crovetto, Francesca Eixarch Roca, Elisenda Rodríguez Sureda, Víctor Domínguez, Carmen Casu, Giulia Rovira, Carlota Nadal Serra, Alfons Crispi Brillas, Fàtima Gratacós Solsona, Eduard |
| author2_role | author author author author author author author author author author author |
| author_browse | Casu, Giulia Crispi Brillas, Fàtima Crovetto, Francesca Dantas, Ana Paula Domínguez, Carmen Eixarch Roca, Elisenda Gratacós Solsona, Eduard Miranda, Jezid Nadal Serra, Alfons Paules, Cristina Rodríguez Sureda, Víctor Rovira, Carlota |
| author_facet | Paules, Cristina Dantas, Ana Paula Miranda, Jezid Crovetto, Francesca Eixarch Roca, Elisenda Rodríguez Sureda, Víctor Domínguez, Carmen Casu, Giulia Rovira, Carlota Nadal Serra, Alfons Crispi Brillas, Fàtima Gratacós Solsona, Eduard |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | Objective The aim of this study was to perform a comprehensive assessment of the placental aging process through senescence and apoptotic markers in late-onset small fetuses classified as SGA or FGR. Study Design A prospective nested case-control study in singleton pregnancies delivering at term including 21 normally grown fetuses and 36 small fetuses classified into SGA (if birthweight was between the 3rd and 9th centile and normal fetoplacental Doppler; n=18) and FGR (if birthweight <3rd centile and/or abnormal cerebroplacental ratio or uterine artery Doppler; n=18). Telomerase activity, telomere length and RNA expression of senescence (Sirtuin 1,3,6) and apoptotic markers (p53, p21, BAX, Caspase 3 and 9) were analyzed in placental samples collected at birth. Results Compared with normally grown fetuses, both SGA and FGR presented signs of accelerated placental aging including lower telomerase activity (controls mean±SD 12.8% ± 6.6 vs SGA 7.98% ± 4.2 vs FGR 7.79% ± 4.6, p=0.008), shorter telomeres (controls 1.20 T/S ± 0.6 vs SGA 1.08 T/S ± 0.9 vs FGR 0.66 T/S ± 0.5, p=0.017), and reduced Sirtuin1 RNA expression (controls 1.55 2-' ' Ct ± 0.8 vs SGA 0.91 2-' ' Ct ± 0.8vs FGR 0.63 2-' ' Ct ± 0.5, p<0.001) together with increased p53 RNA expression (controls median(IQR) 1.072-' ' Ct (3.2) vs SGA 5.39 2-' ' Ct (15) vs FGR 3.75 2-' ' Ct (7.8), p=0.040), with a significant linear tendency across severity stages. In addition, FGR cases presented signs of apoptosis with increased RNA levels of Caspase 3 (controls 0.94 2-' ' Ct (1.1) vs FGR 3.98 2-' ' Ct (30), p=0.031) and Caspase 9 (controls 1.21 2-' ' Ct (4.0) vs FGR 3.87 2-' ' Ct (8.7), p=0.037) as compared to controls. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b68d7b2d82efc1948a3a91dbb47e6653 |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/125602 |
| publishDate | 2018 |
| publishDateSort | 2018 |
| publisher | John Wiley & Sons |
| publisherStr | John Wiley & Sons |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses Paules, Cristina Dantas, Ana Paula Miranda, Jezid Crovetto, Francesca Eixarch Roca, Elisenda Rodríguez Sureda, Víctor Domínguez, Carmen Casu, Giulia Rovira, Carlota Nadal Serra, Alfons Crispi Brillas, Fàtima Gratacós Solsona, Eduard Creixement fetal Placenta Telòmer Fetal growth Telomere Objective The aim of this study was to perform a comprehensive assessment of the placental aging process through senescence and apoptotic markers in late-onset small fetuses classified as SGA or FGR. Study Design A prospective nested case-control study in singleton pregnancies delivering at term including 21 normally grown fetuses and 36 small fetuses classified into SGA (if birthweight was between the 3rd and 9th centile and normal fetoplacental Doppler; n=18) and FGR (if birthweight <3rd centile and/or abnormal cerebroplacental ratio or uterine artery Doppler; n=18). Telomerase activity, telomere length and RNA expression of senescence (Sirtuin 1,3,6) and apoptotic markers (p53, p21, BAX, Caspase 3 and 9) were analyzed in placental samples collected at birth. Results Compared with normally grown fetuses, both SGA and FGR presented signs of accelerated placental aging including lower telomerase activity (controls mean±SD 12.8% ± 6.6 vs SGA 7.98% ± 4.2 vs FGR 7.79% ± 4.6, p=0.008), shorter telomeres (controls 1.20 T/S ± 0.6 vs SGA 1.08 T/S ± 0.9 vs FGR 0.66 T/S ± 0.5, p=0.017), and reduced Sirtuin1 RNA expression (controls 1.55 2-' ' Ct ± 0.8 vs SGA 0.91 2-' ' Ct ± 0.8vs FGR 0.63 2-' ' Ct ± 0.5, p<0.001) together with increased p53 RNA expression (controls median(IQR) 1.072-' ' Ct (3.2) vs SGA 5.39 2-' ' Ct (15) vs FGR 3.75 2-' ' Ct (7.8), p=0.040), with a significant linear tendency across severity stages. In addition, FGR cases presented signs of apoptosis with increased RNA levels of Caspase 3 (controls 0.94 2-' ' Ct (1.1) vs FGR 3.98 2-' ' Ct (30), p=0.031) and Caspase 9 (controls 1.21 2-' ' Ct (4.0) vs FGR 3.87 2-' ' Ct (8.7), p=0.037) as compared to controls. John Wiley & Sons https://hdl.handle.net/2445/125602 |
| spellingShingle | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses Paules, Cristina Creixement fetal Placenta Telòmer Fetal growth Telomere |
| status_str | acceptedVersion |
| title | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses |
| title_full | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses |
| title_fullStr | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses |
| title_full_unstemmed | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses |
| title_short | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses |
| title_sort | Premature placental aging in term small-for-gestational-age and fetal-growth-restricted fetuses |
| topic | Creixement fetal Placenta Telòmer Fetal growth Telomere |
| topic_facet | Creixement fetal Placenta Telòmer Fetal growth Telomere |
| url | https://hdl.handle.net/2445/125602 |