Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients
Brain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and...
| Authors: | ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Universidad de Barcelona |
| Repository: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/199042 |
| Online Access: | https://hdl.handle.net/2445/199042 |
| Access Level: | Open access |
| Keyword: | Envelliment cerebral Hipocamp (Cervell) Malaltia d'Alzheimer Aging brain Hippocampus (Brain) Alzheimer's disease |
| _version_ | 1878738782142857217 |
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| author | Riba Baqués, Marta |
| author2 | Valle i Macià, Jaume del Romera, Clara Alsina Planelles, Raquel Molina Porcel, Laura Pelegrí i Gabaldà, Carme Vilaplana i Hortensi, Jordi |
| author2_role | author author author author author author |
| author_browse | Alsina Planelles, Raquel Molina Porcel, Laura Pelegrí i Gabaldà, Carme Riba Baqués, Marta Romera, Clara Valle i Macià, Jaume del Vilaplana i Hortensi, Jordi |
| author_facet | Riba Baqués, Marta Valle i Macià, Jaume del Romera, Clara Alsina Planelles, Raquel Molina Porcel, Laura Pelegrí i Gabaldà, Carme Vilaplana i Hortensi, Jordi |
| author_role | author |
| collection | Dipòsit Digital de la UB |
| description | Brain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and the presence of tau protein in them has been controversial. In this work, we reanalyzed the presence of this protein in wasteosomes and we pointed out a methodological problem when immunolabeling. It is well known that to detect tau it is necessary to perform an antigen retrieval. However, in the case of wasteosomes, an excessive antigen retrieval with boiling dissolves their polyglucosan structure, releases the entrapped proteins and, thus, prevents their detection. After performing an adequate pre-treatment, with an intermediate time of boiling, we observed that some brain wasteosomes from patients with Alzheimer's disease (AD) contained tau, while we did not detect tau protein in those from non-AD patients. These observations pointed the different composition of wasteosomes depending on the neuropathological condition and reinforce the role of wasteosomes as waste containers. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b509ceede4c046bdccc996f3dcbd2f4a |
| institution | Universidad de Barcelona |
| instname_str | Universidad de Barcelona |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:diposit.ub.edu:2445/199042 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| reponame_str | Dipòsit Digital de la UB |
| spelling | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients Riba Baqués, Marta Valle i Macià, Jaume del Romera, Clara Alsina Planelles, Raquel Molina Porcel, Laura Pelegrí i Gabaldà, Carme Vilaplana i Hortensi, Jordi Envelliment cerebral Hipocamp (Cervell) Malaltia d'Alzheimer Aging brain Hippocampus (Brain) Alzheimer's disease Brain corpora amylacea, recently renamed as wasteosomes, are polyglucosan bodies that appear during aging and some neurodegenerative conditions. They collect waste substances and are part of a brain cleaning mechanism. For decades, studies on their composition have produced inconsistent results and the presence of tau protein in them has been controversial. In this work, we reanalyzed the presence of this protein in wasteosomes and we pointed out a methodological problem when immunolabeling. It is well known that to detect tau it is necessary to perform an antigen retrieval. However, in the case of wasteosomes, an excessive antigen retrieval with boiling dissolves their polyglucosan structure, releases the entrapped proteins and, thus, prevents their detection. After performing an adequate pre-treatment, with an intermediate time of boiling, we observed that some brain wasteosomes from patients with Alzheimer's disease (AD) contained tau, while we did not detect tau protein in those from non-AD patients. These observations pointed the different composition of wasteosomes depending on the neuropathological condition and reinforce the role of wasteosomes as waste containers. Frontiers Media https://hdl.handle.net/2445/199042 |
| spellingShingle | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients Riba Baqués, Marta Envelliment cerebral Hipocamp (Cervell) Malaltia d'Alzheimer Aging brain Hippocampus (Brain) Alzheimer's disease |
| status_str | publishedVersion |
| title | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients |
| title_full | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients |
| title_fullStr | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients |
| title_full_unstemmed | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients |
| title_short | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients |
| title_sort | Uncovering tau in wasteosomes (corpora amylacea) of Alzheimer's disease patients |
| topic | Envelliment cerebral Hipocamp (Cervell) Malaltia d'Alzheimer Aging brain Hippocampus (Brain) Alzheimer's disease |
| topic_facet | Envelliment cerebral Hipocamp (Cervell) Malaltia d'Alzheimer Aging brain Hippocampus (Brain) Alzheimer's disease |
| url | https://hdl.handle.net/2445/199042 |