Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia
Strokes are the second most common cause of death worldwide and a leading cause of disability. Regular consumption of polyphenols has been shown to reduce the risk of suffering a cardiovascular event. For this reason, we have investigated the protective effect of Salicornia ramosissima, a seasonal h...
| Authors: | ; ; ; ; ; ; |
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| Format: | Article |
| Status: | Published version |
| Publication date: | 2022 |
| Country: | España |
| Institution: | Universidad de Sevilla (US) |
| Repository: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/170131 |
| Online Access: | https://hdl.handle.net/11441/170131 https://doi.org/10.3390/nu14235077 |
| Access Level: | Open access |
| Keyword: | Salicornia stroke neuroprotection polyphenol ischemia |
| _version_ | 1878738761104228352 |
|---|---|
| author | García Rodríguez, Paula |
| author2 | Ma, Feifei Río Mercado, Carmen del Romero Bernal, Marina Nájar Moyano, Ana María Cádiz Gurrea, María de la Luz Montaner, Joan |
| author2_role | author author author author author author |
| author_browse | Cádiz Gurrea, María de la Luz García Rodríguez, Paula Ma, Feifei Montaner, Joan Nájar Moyano, Ana María Romero Bernal, Marina Río Mercado, Carmen del |
| author_facet | García Rodríguez, Paula Ma, Feifei Río Mercado, Carmen del Romero Bernal, Marina Nájar Moyano, Ana María Cádiz Gurrea, María de la Luz Montaner, Joan |
| author_role | author |
| collection | idUS. Depósito de Investigación de la Universidad de Sevilla |
| description | Strokes are the second most common cause of death worldwide and a leading cause of disability. Regular consumption of polyphenols has been shown to reduce the risk of suffering a cardiovascular event. For this reason, we have investigated the protective effect of Salicornia ramosissima, a seasonal halophyte that synthetizes high amounts of bioactive compounds, including polyphenols, in response to environmental stress. Aqueous, hydroalcoholic, and ethanolic extracts were prepared to investigate if dietary supplementation prior to ischemic challenge can prevent subsequent damage using two animal models. First, we screened the protective effect against hypoxia–reoxygenation in Drosophila melanogaster and observed that both ethanolic and hydroalcoholic extracts protected flies from the deleterious effects of hypoxia. Second, we confirmed the protective effect of S. ramosissima ethanolic extract against brain ischemia using the transient middle cerebral artery occlusion mice model. Four weeks of oral supplementation with the ethanolic extract before artery occlusion reduced infarct volume and lowered the plasma levels of the DNA peroxidant product 8-hydroxydeoxyguanosine. Phytochemical profiling of S. ramosissima ethanolic extract revealed 50 compounds. Thus, it represents a valuable source of bioactive compounds that show promising disease-modifying activities and could be further developed as an effective food supplement for the prevention or treatment of neurovascular disorders. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b4ab8578a4fd14dbdc3ef6afd8f5bff2 |
| institution | Universidad de Sevilla (US) |
| instname_str | Universidad de Sevilla (US) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:idus.us.es:11441/170131 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | idUS. Depósito de Investigación de la Universidad de Sevilla |
| spelling | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia García Rodríguez, Paula Ma, Feifei Río Mercado, Carmen del Romero Bernal, Marina Nájar Moyano, Ana María Cádiz Gurrea, María de la Luz Montaner, Joan Salicornia stroke neuroprotection polyphenol ischemia Strokes are the second most common cause of death worldwide and a leading cause of disability. Regular consumption of polyphenols has been shown to reduce the risk of suffering a cardiovascular event. For this reason, we have investigated the protective effect of Salicornia ramosissima, a seasonal halophyte that synthetizes high amounts of bioactive compounds, including polyphenols, in response to environmental stress. Aqueous, hydroalcoholic, and ethanolic extracts were prepared to investigate if dietary supplementation prior to ischemic challenge can prevent subsequent damage using two animal models. First, we screened the protective effect against hypoxia–reoxygenation in Drosophila melanogaster and observed that both ethanolic and hydroalcoholic extracts protected flies from the deleterious effects of hypoxia. Second, we confirmed the protective effect of S. ramosissima ethanolic extract against brain ischemia using the transient middle cerebral artery occlusion mice model. Four weeks of oral supplementation with the ethanolic extract before artery occlusion reduced infarct volume and lowered the plasma levels of the DNA peroxidant product 8-hydroxydeoxyguanosine. Phytochemical profiling of S. ramosissima ethanolic extract revealed 50 compounds. Thus, it represents a valuable source of bioactive compounds that show promising disease-modifying activities and could be further developed as an effective food supplement for the prevention or treatment of neurovascular disorders. CSF-Proyectos estratégicos de I+D+i.y Programa Operativo FEDER de Andalucía 2014–2020 PE-0527-2019 Consejería de Transformación Económica, Industria, Conocimiento y Universidades de la Junta de Andalucía (CTEICU) y UE, PO FEDER Andalucía 2014–2020 PY20_01351 MDPI https://hdl.handle.net/11441/170131 https://doi.org/10.3390/nu14235077 |
| spellingShingle | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia García Rodríguez, Paula Salicornia stroke neuroprotection polyphenol ischemia |
| status_str | publishedVersion |
| title | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_full | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_fullStr | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_full_unstemmed | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_short | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| title_sort | Diet Supplementation with Polyphenol-Rich Salicornia ramosissima Extracts Protects against Tissue Damage in Experimental Models of Cerebral Ischemia |
| topic | Salicornia stroke neuroprotection polyphenol ischemia |
| topic_facet | Salicornia stroke neuroprotection polyphenol ischemia |
| url | https://hdl.handle.net/11441/170131 https://doi.org/10.3390/nu14235077 |