Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis
Datos de investigación alojados en: https://doi.org/10.5281/zenodo.16083355
| Authors: | ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2023 |
| Country: | España |
| Institution: | Universidad Pública de Navarra |
| Repository: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/46453 |
| Online Access: | https://hdl.handle.net/2454/46453 |
| Access Level: | Open access |
| Keyword: | Cysteine proteases Amaranthus palmeri Glyphosate Pyrithiobac Amino acid biosynthesis |
| _version_ | 1878738708392312832 |
|---|---|
| author | Barco Antoñanzas, María |
| author2 | Font Farre, María Eceiza, Mikel Vicente Gil Monreal, Miriam Van der Hoorn, Reiner Royuela Hernando, Mercedes Zabalza Aznárez, Ana |
| author2_role | author author author author author author |
| author_browse | Barco Antoñanzas, María Eceiza, Mikel Vicente Font Farre, María Gil Monreal, Miriam Royuela Hernando, Mercedes Van der Hoorn, Reiner Zabalza Aznárez, Ana |
| author_facet | Barco Antoñanzas, María Font Farre, María Eceiza, Mikel Vicente Gil Monreal, Miriam Van der Hoorn, Reiner Royuela Hernando, Mercedes Zabalza Aznárez, Ana |
| author_role | author |
| collection | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| description | Datos de investigación alojados en: https://doi.org/10.5281/zenodo.16083355 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_b3aa2cd2bf697c56347eba1c778ffc79 |
| institution | Universidad Pública de Navarra |
| instname_str | Universidad Pública de Navarra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:academica-e.unavarra.es:2454/46453 |
| publishDate | 2023 |
| publishDateSort | 2023 |
| publisher | Wiley |
| publisherStr | Wiley |
| reponame_str | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| spelling | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis Barco Antoñanzas, María Font Farre, María Eceiza, Mikel Vicente Gil Monreal, Miriam Van der Hoorn, Reiner Royuela Hernando, Mercedes Zabalza Aznárez, Ana Cysteine proteases Amaranthus palmeri Glyphosate Pyrithiobac Amino acid biosynthesis Datos de investigación alojados en: https://doi.org/10.5281/zenodo.16083355 The herbicides glyphosate and pyrithiobac inhibit the enzyme 5-enolpyruvylshikimate3-phosphate synthase (EPSPS) in the aromatic amino acid biosynthetic pathway and acetolactate synthase (ALS) in the branched-chain amino acid biosynthetic pathway, respectively. Here we characterise the protease activity profiles of a sensitive (S), a glyphosate-resistant (GR) and a multiple-resistant (MR) population of Amaranthus palmeri in response to glyphosate and pyrithiobac. Amino acid accumulation and cysteine protease activities were induced with both herbicides in the S population and with pyrithiobac in the GR population, suggesting that the increase in cysteine proteases is responsible for the increased degradation of the available proteins and the observed increase in free amino acids. Herbicides did not induce any changes in the proteolytic activities in the populations with target-site resistance, indicating that this effect was only induced in sensitive plants. This work was funded by the Spanish Ministry of Economy and Competitiveness (AGL2016-77531-R) and Spanish Ministry of Science and Innovation (2020 117723-RB-100). Maria Barco-Antoñanzas received funding from PhD fellowship and mobility grant through Universidad Pública de Navarra. Mikel V. Eceiza is the holder of a predoctoral fellowship of the Basque Government. We thank Gustavo Garijo and Clara Jimenez for technical assistance. Open access funding provided by Universidad Pública de Navarra. Wiley https://hdl.handle.net/2454/46453 |
| spellingShingle | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis Barco Antoñanzas, María Cysteine proteases Amaranthus palmeri Glyphosate Pyrithiobac Amino acid biosynthesis |
| status_str | publishedVersion |
| title | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis |
| title_full | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis |
| title_fullStr | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis |
| title_full_unstemmed | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis |
| title_short | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis |
| title_sort | Cysteine proteases are activated in sensitive Amaranthus palmeri populations upon treatment with herbicides inhibiting amino acid biosynthesis |
| topic | Cysteine proteases Amaranthus palmeri Glyphosate Pyrithiobac Amino acid biosynthesis |
| topic_facet | Cysteine proteases Amaranthus palmeri Glyphosate Pyrithiobac Amino acid biosynthesis |
| url | https://hdl.handle.net/2454/46453 |