Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation

[EN] The hydrolysis of legume proteins improves their nutritional and functional properties. Usually done by mixing flour with an enzyme solution, the process can be simplified using vacuum enzyme impregnation during soaking. This study used vacuum impregnation with papain or bromelain to obtain hyd...

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Authors: Arnal-Salinas, Milagros|||0000-0002-9301-2478; Talens Oliag, Pau|||0000-0001-7318-3336; Gallego Ibáñez, Marta; Mora Soler, Leticia
Format: Article
Publication date:2025
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/214070
Online Access:https://riunet.upv.es/handle/10251/214070
Access Level:Open access
Keyword:Legumes
Hydrolysed flours
Vacuum impregnation soaking
Papain
Bromelain
TECNOLOGIA DE ALIMENTOS
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author Arnal-Salinas, Milagros|||0000-0002-9301-2478
author2 Talens Oliag, Pau|||0000-0001-7318-3336
Gallego Ibáñez, Marta
Mora Soler, Leticia
author2_role author
author
author
author_browse Arnal-Salinas, Milagros|||0000-0002-9301-2478
Gallego Ibáñez, Marta
Mora Soler, Leticia
Talens Oliag, Pau|||0000-0001-7318-3336
author_facet Arnal-Salinas, Milagros|||0000-0002-9301-2478
Talens Oliag, Pau|||0000-0001-7318-3336
Gallego Ibáñez, Marta
Mora Soler, Leticia
author_role author
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
description [EN] The hydrolysis of legume proteins improves their nutritional and functional properties. Usually done by mixing flour with an enzyme solution, the process can be simplified using vacuum enzyme impregnation during soaking. This study used vacuum impregnation with papain or bromelain to obtain hydrolysed broad bean flours. It examined the impact of vacuum impregnation and enzyme incorporation on hydration kinetics and the impact of hydrolysis and dehulling on antinutritional factors and protein digestibility. Vacuum impregnation accelerated hydration and enzyme incorporation did not alter the hydration rate. Maximum hydrolysis degrees were 9.1 % with papain and 8.8 % with bromelain after 4 h of soaking. Hydrolysis increased phytic acid, total phenolics, and tannins content while decreasing trypsin inhibitors. Dehulling increased phytic acid and trypsin inhibitors, reduced tannins, and enhanced protein digestibility. Vacuum enzyme impregnation during soaking was effective for hydration and protein hydrolysis, modifying the nutritional properties of broad bean flours.
eu_rights_str_mv openAccess
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institution Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
language eng
network_acronym_str ES
network_name_str España
oai_identifier_str oai:riunet.upv.es:10251/214070
publishDate 2025
publishDateSort 2025
publisher Elsevier
publisherStr Elsevier
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
spelling Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation Arnal-Salinas, Milagros|||0000-0002-9301-2478 Talens Oliag, Pau|||0000-0001-7318-3336 Gallego Ibáñez, Marta Mora Soler, Leticia Legumes Hydrolysed flours Vacuum impregnation soaking Papain Bromelain TECNOLOGIA DE ALIMENTOS [EN] The hydrolysis of legume proteins improves their nutritional and functional properties. Usually done by mixing flour with an enzyme solution, the process can be simplified using vacuum enzyme impregnation during soaking. This study used vacuum impregnation with papain or bromelain to obtain hydrolysed broad bean flours. It examined the impact of vacuum impregnation and enzyme incorporation on hydration kinetics and the impact of hydrolysis and dehulling on antinutritional factors and protein digestibility. Vacuum impregnation accelerated hydration and enzyme incorporation did not alter the hydration rate. Maximum hydrolysis degrees were 9.1 % with papain and 8.8 % with bromelain after 4 h of soaking. Hydrolysis increased phytic acid, total phenolics, and tannins content while decreasing trypsin inhibitors. Dehulling increased phytic acid and trypsin inhibitors, reduced tannins, and enhanced protein digestibility. Vacuum enzyme impregnation during soaking was effective for hydration and protein hydrolysis, modifying the nutritional properties of broad bean flours. M.A. gratefully acknowledge the Predoctoral contract (FPU19/02401) from the Spanish Ministry of Science, Innovation and Universities. Elsevier https://riunet.upv.es/handle/10251/214070
spellingShingle Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
Arnal-Salinas, Milagros|||0000-0002-9301-2478
Legumes
Hydrolysed flours
Vacuum impregnation soaking
Papain
Bromelain
TECNOLOGIA DE ALIMENTOS
title Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
title_full Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
title_fullStr Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
title_full_unstemmed Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
title_short Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
title_sort Antinutritional factors and protein digestibility of broad bean flours hydrolysed during soaking using vacuum enzyme impregnation
topic Legumes
Hydrolysed flours
Vacuum impregnation soaking
Papain
Bromelain
TECNOLOGIA DE ALIMENTOS
topic_facet Legumes
Hydrolysed flours
Vacuum impregnation soaking
Papain
Bromelain
TECNOLOGIA DE ALIMENTOS
url https://riunet.upv.es/handle/10251/214070