Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients
Mycobacterium abscessus is an extensively drug-resistant pathogen that causes pulmonary disease, particularly in cystic fibrosis (CF) patients. Identifying direct patient-to-patient transmission of M. abscessus is critically important in directing an infection control policy for the management of ri...
| Autores: | ; ; ; ; ; ; |
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| Tipo de documento: | Artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:ddd.uab.cat:226508 |
| Acceso en línea: | https://ddd.uab.cat/record/226508 https://dx.doi.org/urn:doi:10.1093/cid/ciz526 |
| Nivel de acceso: | Acceso abierto |
| Palabra clave: | Nontuberculous mycobacteria Whole-genome sequencing Transmission Cystic fibrosis Phylogenomics |
| _version_ | 1878738321392271360 |
|---|---|
| author | Doyle, Ronan M.|||0000-0001-5001-1945 |
| author2 | Rubio, Marc|||0000-0002-4811-0642 Dixon, Garth|||0000-0001-8165-3094 Hartley, John Klein, Nigel Coll Figa, Pedro|||0000-0003-1782-9782 Harris, Kathryn A. |
| author2_role | author author author author author author |
| author_browse | Coll Figa, Pedro|||0000-0003-1782-9782 Dixon, Garth|||0000-0001-8165-3094 Doyle, Ronan M.|||0000-0001-5001-1945 Harris, Kathryn A. Hartley, John Klein, Nigel Rubio, Marc|||0000-0002-4811-0642 |
| author_facet | Doyle, Ronan M.|||0000-0001-5001-1945 Rubio, Marc|||0000-0002-4811-0642 Dixon, Garth|||0000-0001-8165-3094 Hartley, John Klein, Nigel Coll Figa, Pedro|||0000-0003-1782-9782 Harris, Kathryn A. |
| author_role | author |
| collection | Dipòsit Digital de Documents de la UAB |
| description | Mycobacterium abscessus is an extensively drug-resistant pathogen that causes pulmonary disease, particularly in cystic fibrosis (CF) patients. Identifying direct patient-to-patient transmission of M. abscessus is critically important in directing an infection control policy for the management of risk in CF patients. A variety of clinical labs have used molecular epidemiology to investigate transmission. However, there is still conflicting evidence as to how M. abscessus is acquired and whether cross-transmission occurs. Recently, labs have applied whole-genome sequencing (WGS) to investigate this further and, in this study, we investigated whether WGS can reliably identify cross-transmission in M. abscessus. We retrospectively sequenced the whole genomes of 145 M. abscessus isolates from 62 patients, seen at 4 hospitals in 2 countries over 16 years. We have shown that a comparison of a fixed number of core single nucleotide variants alone cannot be used to infer cross-transmission in M. abscessus but does provide enough information to replace multiple existing molecular assays. We detected 1 episode of possible direct patient-to-patient transmission in a sibling pair. We found that patients acquired unique M. abscessus strains even after spending considerable time on the same wards with other M. abscessus- positive patients. This novel analysis has demonstrated that the majority of patients in this study have not acquired M. abscessus through direct patient-to-patient transmission or a common reservoir. Tracking transmission using WGS will only realize its full potential with proper environmental screening, as well as patient sampling. Whole-genome sequencing should replace the current molecular typing used routinely in clinical microbiology laboratories. The patient-to-patient spread of Mycobacterium abscessus is not common. Environmental screening may provide a better understanding of the acquisition of M. abscessus infections. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_ad1de5b4dcc3577eeb5478fca3b2dccd |
| institution | Universitat Autònoma de Barcelona |
| instname_str | Universitat Autònoma de Barcelona |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:ddd.uab.cat:226508 |
| publishDate | 2019 |
| publishDateSort | 2019 |
| reponame_str | Dipòsit Digital de Documents de la UAB |
| spelling | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients Doyle, Ronan M.|||0000-0001-5001-1945 Rubio, Marc|||0000-0002-4811-0642 Dixon, Garth|||0000-0001-8165-3094 Hartley, John Klein, Nigel Coll Figa, Pedro|||0000-0003-1782-9782 Harris, Kathryn A. Nontuberculous mycobacteria Whole-genome sequencing Transmission Cystic fibrosis Phylogenomics Mycobacterium abscessus is an extensively drug-resistant pathogen that causes pulmonary disease, particularly in cystic fibrosis (CF) patients. Identifying direct patient-to-patient transmission of M. abscessus is critically important in directing an infection control policy for the management of risk in CF patients. A variety of clinical labs have used molecular epidemiology to investigate transmission. However, there is still conflicting evidence as to how M. abscessus is acquired and whether cross-transmission occurs. Recently, labs have applied whole-genome sequencing (WGS) to investigate this further and, in this study, we investigated whether WGS can reliably identify cross-transmission in M. abscessus. We retrospectively sequenced the whole genomes of 145 M. abscessus isolates from 62 patients, seen at 4 hospitals in 2 countries over 16 years. We have shown that a comparison of a fixed number of core single nucleotide variants alone cannot be used to infer cross-transmission in M. abscessus but does provide enough information to replace multiple existing molecular assays. We detected 1 episode of possible direct patient-to-patient transmission in a sibling pair. We found that patients acquired unique M. abscessus strains even after spending considerable time on the same wards with other M. abscessus- positive patients. This novel analysis has demonstrated that the majority of patients in this study have not acquired M. abscessus through direct patient-to-patient transmission or a common reservoir. Tracking transmission using WGS will only realize its full potential with proper environmental screening, as well as patient sampling. Whole-genome sequencing should replace the current molecular typing used routinely in clinical microbiology laboratories. The patient-to-patient spread of Mycobacterium abscessus is not common. Environmental screening may provide a better understanding of the acquisition of M. abscessus infections. https://ddd.uab.cat/record/226508 https://dx.doi.org/urn:doi:10.1093/cid/ciz526 |
| spellingShingle | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients Doyle, Ronan M.|||0000-0001-5001-1945 Nontuberculous mycobacteria Whole-genome sequencing Transmission Cystic fibrosis Phylogenomics |
| title | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients |
| title_full | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients |
| title_fullStr | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients |
| title_full_unstemmed | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients |
| title_short | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients |
| title_sort | Cross-transmission Is Not the Source of New Mycobacterium abscessus Infections in a Multicenter Cohort of Cystic Fibrosis Patients |
| topic | Nontuberculous mycobacteria Whole-genome sequencing Transmission Cystic fibrosis Phylogenomics |
| topic_facet | Nontuberculous mycobacteria Whole-genome sequencing Transmission Cystic fibrosis Phylogenomics |
| url | https://ddd.uab.cat/record/226508 https://dx.doi.org/urn:doi:10.1093/cid/ciz526 |