Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis
Inflammatory activity in rheumatoid arthritis may alter the regulation of muscle mass leading to a secondary sarcopenia, commonly termed rheumatoid cachexia (RC). We characterized alterations to muscle structure and various pro-inflammatory, catabolic and regenerative markers in an animal model of R...
| Authors: | ; ; ; ; ; ; ; |
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| Format: | Article |
| Publication date: | 2017 |
| Country: | España |
| Institution: | Universidad Autónoma de Madrid |
| Repository: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Language: | English |
| OAI Identifier: | oai:repositorio.uam.es:10486/680224 |
| Online Access: | http://hdl.handle.net/10486/680224 https://dx.doi.org/10.1038/s41598-017-06581-6 |
| Access Level: | Open access |
| Keyword: | Arthritis Sarcopenia Chronic arthritis Compensatory anabolic Medicina |
| _version_ | 1878738251107270656 |
|---|---|
| author | Little, Robert D. |
| author2 | Prieto-Potin, Iván Pérez-Baos, Sandra Villalvilla, Amanda Gratal, Paula Cicuttini, Flavia Largo, Raquel Herrero-Beaumont Cuenca, Gabriel |
| author2_role | author author author author author author author |
| author_browse | Cicuttini, Flavia Gratal, Paula Herrero-Beaumont Cuenca, Gabriel Largo, Raquel Little, Robert D. Prieto-Potin, Iván Pérez-Baos, Sandra Villalvilla, Amanda |
| author_facet | Little, Robert D. Prieto-Potin, Iván Pérez-Baos, Sandra Villalvilla, Amanda Gratal, Paula Cicuttini, Flavia Largo, Raquel Herrero-Beaumont Cuenca, Gabriel |
| author_role | author |
| collection | Biblos-e Archivo. Repositorio Institucional de la UAM |
| description | Inflammatory activity in rheumatoid arthritis may alter the regulation of muscle mass leading to a secondary sarcopenia, commonly termed rheumatoid cachexia (RC). We characterized alterations to muscle structure and various pro-inflammatory, catabolic and regenerative markers in an animal model of RC. Antigen induced arthritis (AiA) was performed in 20 male adult rabbits. AiA animals exhibited significantly less weight gain, a markedly elevated serum C-reactive protein (CRP), lighter muscles with shorter cross-sectional diameter and increased myonuclei when compared to controls. Atrogin-1 and MuRF-1 were up-regulated alongside an increase in IL-1β, active NF-κB and a higher ratio of phosphorylated to inactive p38 MAPK. CCL-2 and TNF levels were reduced and IL-6 was unchanged between groups. We observed decreased pSTAT3, unchanged pSTAT1 and Myf5, but increased Pax7, MyoD and myogenin. AiA rabbits had a reduction in myostatin from gastrocnemii and synovium with a congruent decrease in serum myostatin compared to controls. Chronic arthritis induced an RC-like secondary sarcopenia with increased muscle protein breakdown. Elevated IL-1β may trigger proteolysis via elevated NF-κB and p38 MAPK signaling with a compensatory anabolic response suggested by myonuclear expansion, increased Pax7, MyoD and myogenin, reduced pSTAT3 as well as reduced serum, synovial and muscular myostatin |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_abf09eaef97c833aeeb4ae5cfaa0e4cb |
| institution | Universidad Autónoma de Madrid |
| instname_str | Universidad Autónoma de Madrid |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repositorio.uam.es:10486/680224 |
| publishDate | 2017 |
| publishDateSort | 2017 |
| publisher | Nature Publishing Group |
| publisherStr | Nature Publishing Group |
| reponame_str | Biblos-e Archivo. Repositorio Institucional de la UAM |
| spelling | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis Little, Robert D. Prieto-Potin, Iván Pérez-Baos, Sandra Villalvilla, Amanda Gratal, Paula Cicuttini, Flavia Largo, Raquel Herrero-Beaumont Cuenca, Gabriel Arthritis Sarcopenia Chronic arthritis Compensatory anabolic Medicina Inflammatory activity in rheumatoid arthritis may alter the regulation of muscle mass leading to a secondary sarcopenia, commonly termed rheumatoid cachexia (RC). We characterized alterations to muscle structure and various pro-inflammatory, catabolic and regenerative markers in an animal model of RC. Antigen induced arthritis (AiA) was performed in 20 male adult rabbits. AiA animals exhibited significantly less weight gain, a markedly elevated serum C-reactive protein (CRP), lighter muscles with shorter cross-sectional diameter and increased myonuclei when compared to controls. Atrogin-1 and MuRF-1 were up-regulated alongside an increase in IL-1β, active NF-κB and a higher ratio of phosphorylated to inactive p38 MAPK. CCL-2 and TNF levels were reduced and IL-6 was unchanged between groups. We observed decreased pSTAT3, unchanged pSTAT1 and Myf5, but increased Pax7, MyoD and myogenin. AiA rabbits had a reduction in myostatin from gastrocnemii and synovium with a congruent decrease in serum myostatin compared to controls. Chronic arthritis induced an RC-like secondary sarcopenia with increased muscle protein breakdown. Elevated IL-1β may trigger proteolysis via elevated NF-κB and p38 MAPK signaling with a compensatory anabolic response suggested by myonuclear expansion, increased Pax7, MyoD and myogenin, reduced pSTAT3 as well as reduced serum, synovial and muscular myostatin This work was partially supported by research grants from the Instituto de Salud Carlos III (PI13/00570, PI15/00340, PI16/00065 and RETICEF RD12/0043/0008), co-funded by Fondo Europeo de Desarrollo Regional (FEDER) and ASPIRE IIR grant from Pfizer. Nature Publishing Group http://hdl.handle.net/10486/680224 https://dx.doi.org/10.1038/s41598-017-06581-6 |
| spellingShingle | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis Little, Robert D. Arthritis Sarcopenia Chronic arthritis Compensatory anabolic Medicina |
| title | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis |
| title_full | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis |
| title_fullStr | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis |
| title_full_unstemmed | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis |
| title_short | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis |
| title_sort | Compensatory anabolic signaling in the sarcopenia of experimental chronic arthritis |
| topic | Arthritis Sarcopenia Chronic arthritis Compensatory anabolic Medicina |
| topic_facet | Arthritis Sarcopenia Chronic arthritis Compensatory anabolic Medicina |
| url | http://hdl.handle.net/10486/680224 https://dx.doi.org/10.1038/s41598-017-06581-6 |