Tryptophan levels as a marker of auxins and nitric oxide signaling
The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to pe...
| Authors: | ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Status: | Published version |
| Publication date: | 2022 |
| Country: | España |
| Institution: | Universidad Pública de Navarra |
| Repository: | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| OAI Identifier: | oai:academica-e.unavarra.es:2454/43020 |
| Online Access: | https://hdl.handle.net/2454/43020 |
| Access Level: | Open access |
| Keyword: | Tryptophan Indole-3-acetaldoxime IAOx Nitric oxide NO Stress Principal-component analysis |
| _version_ | 1878737900277858304 |
|---|---|
| author | López Gómez, Pedro |
| author2 | Smith, Edward N. Bota, Pedro Cornejo Ibergallartu, Alfonso Urra, Marina Buezo Bravo, Javier Morán Juez, José Fernando |
| author2_role | author author author author author author |
| author_browse | Bota, Pedro Buezo Bravo, Javier Cornejo Ibergallartu, Alfonso López Gómez, Pedro Morán Juez, José Fernando Smith, Edward N. Urra, Marina |
| author_facet | López Gómez, Pedro Smith, Edward N. Bota, Pedro Cornejo Ibergallartu, Alfonso Urra, Marina Buezo Bravo, Javier Morán Juez, José Fernando |
| author_role | author |
| collection | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| description | The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal component analysis (PCA) was performed on N-metabolism-related compounds, as classified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls and treatments are mainly explained by the differences in Trp contents, which are much higher in controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass-identified compounds between GSNO- and IAOx-treated cells are mainly explained by their concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic acid, which is related to glutathione. These results show that the contents in Trp can be a marker for the study of auxin and NO signaling. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_a6a0cc94ce868febfba2f6a5ca42e092 |
| institution | Universidad Pública de Navarra |
| instname_str | Universidad Pública de Navarra |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:academica-e.unavarra.es:2454/43020 |
| publishDate | 2022 |
| publishDateSort | 2022 |
| publisher | MDPI |
| publisherStr | MDPI |
| reponame_str | Academica-e. Repositorio Institucional de la Universidad Pública de Navarra |
| spelling | Tryptophan levels as a marker of auxins and nitric oxide signaling López Gómez, Pedro Smith, Edward N. Bota, Pedro Cornejo Ibergallartu, Alfonso Urra, Marina Buezo Bravo, Javier Morán Juez, José Fernando Tryptophan Indole-3-acetaldoxime IAOx Nitric oxide NO Stress Principal-component analysis The aromatic amino acid tryptophan is the main precursor for indole-3-acetic acid (IAA), which involves various parallel routes in plants, with indole-3-acetaldoxime (IAOx) being one of the most common intermediates. Auxin signaling is well known to interact with free radical nitric oxide (NO) to perform a more complex effect, including the regulation of root organogenesis and nitrogen nutrition. To fathom the link between IAA and NO, we use a metabolomic approach to analyze the contents of low-molecular-mass molecules in cultured cells of Arabidopsis thaliana after the application of S-nitrosoglutathione (GSNO), an NO donor or IAOx. We separated the crude extracts of the plant cells through ion-exchange columns, and subsequent fractions were analyzed by gas chromatography-mass spectrometry (GC-MS), thus identifying 26 compounds. A principal component analysis (PCA) was performed on N-metabolism-related compounds, as classified by the Kyoto Encyclopedia of Genes and Genomes (KEGG). The differences observed between controls and treatments are mainly explained by the differences in Trp contents, which are much higher in controls. Thus, the Trp is a shared response in both auxin- and NO-mediated signaling, evidencing some common signaling mechanism to both GSNO and IAOx. The differences in the low-molecularmass-identified compounds between GSNO- and IAOx-treated cells are mainly explained by their concentrations in benzenepropanoic acid, which is highly associated with IAA levels, and salicylic acid, which is related to glutathione. These results show that the contents in Trp can be a marker for the study of auxin and NO signaling. This work was supported by grants from the Spanish Ministry of Science and Innovation (AGL2017-52396-P, co-financed by the European Regional Development Fund, FEDER) and a grant from the Public University of Navarre (Res 309/2022) to J.F.M. M.U. is a recipient of a pre-doctoral fellowship from the Government of Navarre, Spain. J.B. and P.L. have received pre-doctoral fellow-ships from the Public University of Navarre, Spain. J.B. was supported by the Romanian Ministry of Education and research, grant CNCS-UEFISCDI, project number PN-III-P4-ID-PCE-2020-2696, within PNCDI III. MDPI https://hdl.handle.net/2454/43020 |
| spellingShingle | Tryptophan levels as a marker of auxins and nitric oxide signaling López Gómez, Pedro Tryptophan Indole-3-acetaldoxime IAOx Nitric oxide NO Stress Principal-component analysis |
| status_str | publishedVersion |
| title | Tryptophan levels as a marker of auxins and nitric oxide signaling |
| title_full | Tryptophan levels as a marker of auxins and nitric oxide signaling |
| title_fullStr | Tryptophan levels as a marker of auxins and nitric oxide signaling |
| title_full_unstemmed | Tryptophan levels as a marker of auxins and nitric oxide signaling |
| title_short | Tryptophan levels as a marker of auxins and nitric oxide signaling |
| title_sort | Tryptophan levels as a marker of auxins and nitric oxide signaling |
| topic | Tryptophan Indole-3-acetaldoxime IAOx Nitric oxide NO Stress Principal-component analysis |
| topic_facet | Tryptophan Indole-3-acetaldoxime IAOx Nitric oxide NO Stress Principal-component analysis |
| url | https://hdl.handle.net/2454/43020 |