Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes.
A primary motivation for sequencing the mouse genome was to accelerate the discovery of mammalian genes by using sequence conservation between mouse and human to identify coding exons. Achieving this goal proved challenging because of the large proportion of the mouse and human genomes that is appar...
| Autores: | ; ; ; ; ; ; ; ; ; ; ; |
|---|---|
| Tipo de documento: | Artigo |
| Estado: | Published version |
| Data de publicação: | 2003 |
| País: | España |
| Recursos: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositório: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/192641 |
| Acesso em linha: | https://hdl.handle.net/2445/192641 |
| Nível de acesso: | Acesso aberto |
| Palavra-chave: | Mamífers Gens Rates (Animals de laboratori) Mammals Genes Rats as laboratory animals |
| _version_ | 1878737319150747648 |
|---|---|
| author | Guigó, Roderic |
| author2 | Dermitzakis, E.T. Agarwal, Pankaj Ponting, Chris P Parra, Genís Reymond, Alexandre Abril Ferrando, Josep Francesc, 1970- Keibler, Evan Lyle, Robert Ucla, Catherine Antonarakis, Stylianos E. Brent, Michael R. |
| author2_role | author author author author author author author author author author author |
| author_browse | Abril Ferrando, Josep Francesc, 1970- Agarwal, Pankaj Antonarakis, Stylianos E. Brent, Michael R. Dermitzakis, E.T. Guigó, Roderic Keibler, Evan Lyle, Robert Parra, Genís Ponting, Chris P Reymond, Alexandre Ucla, Catherine |
| author_facet | Guigó, Roderic Dermitzakis, E.T. Agarwal, Pankaj Ponting, Chris P Parra, Genís Reymond, Alexandre Abril Ferrando, Josep Francesc, 1970- Keibler, Evan Lyle, Robert Ucla, Catherine Antonarakis, Stylianos E. Brent, Michael R. |
| author_role | author |
| collection | Recercat. Dipósit de la Recerca de Catalunya |
| description | A primary motivation for sequencing the mouse genome was to accelerate the discovery of mammalian genes by using sequence conservation between mouse and human to identify coding exons. Achieving this goal proved challenging because of the large proportion of the mouse and human genomes that is apparently conserved but apparently does not code for protein. We developed a two-stage procedure that exploits the mouse and human genome sequences to produce a set of genes with a much higher rate of experimental verification than previously reported prediction methods. RT-PCR amplification and direct sequencing applied to an initial sample of mouse predictions that do not overlap previously known genes verified the regions flanking one intron in 139 predictions, with verification rates reaching 76%. On average, the confirmed predictions show more restricted expression patterns than the mouse orthologs of known human genes, and two-thirds lack homologs in fish genomes, demonstrating the sensitivity of this dual-genome approach to hard-to-find genes. We verified 112 previously unknown homologs of known proteins, including two homeobox proteins relevant to developmental biology, an aquaporin, and a homolog of dystrophin. We estimate that transcription and splicing can be verified for >1,000 gene predictions identified by this method that do not overlap known genes. This is likely to constitute a significant fraction of the previously unknown, multiexon mammalian genes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_9e021e5d347ded8302d4ec6f0252050d |
| institution | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:recercat.cat:2445/192641 |
| publishDate | 2003 |
| publishDateSort | 2003 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| reponame_str | Recercat. Dipósit de la Recerca de Catalunya |
| spelling | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. Guigó, Roderic Dermitzakis, E.T. Agarwal, Pankaj Ponting, Chris P Parra, Genís Reymond, Alexandre Abril Ferrando, Josep Francesc, 1970- Keibler, Evan Lyle, Robert Ucla, Catherine Antonarakis, Stylianos E. Brent, Michael R. Mamífers Gens Rates (Animals de laboratori) Mammals Genes Rats as laboratory animals A primary motivation for sequencing the mouse genome was to accelerate the discovery of mammalian genes by using sequence conservation between mouse and human to identify coding exons. Achieving this goal proved challenging because of the large proportion of the mouse and human genomes that is apparently conserved but apparently does not code for protein. We developed a two-stage procedure that exploits the mouse and human genome sequences to produce a set of genes with a much higher rate of experimental verification than previously reported prediction methods. RT-PCR amplification and direct sequencing applied to an initial sample of mouse predictions that do not overlap previously known genes verified the regions flanking one intron in 139 predictions, with verification rates reaching 76%. On average, the confirmed predictions show more restricted expression patterns than the mouse orthologs of known human genes, and two-thirds lack homologs in fish genomes, demonstrating the sensitivity of this dual-genome approach to hard-to-find genes. We verified 112 previously unknown homologs of known proteins, including two homeobox proteins relevant to developmental biology, an aquaporin, and a homolog of dystrophin. We estimate that transcription and splicing can be verified for >1,000 gene predictions identified by this method that do not overlap known genes. This is likely to constitute a significant fraction of the previously unknown, multiexon mammalian genes. National Academy of Sciences https://hdl.handle.net/2445/192641 |
| spellingShingle | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. Guigó, Roderic Mamífers Gens Rates (Animals de laboratori) Mammals Genes Rats as laboratory animals |
| status_str | publishedVersion |
| title | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. |
| title_full | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. |
| title_fullStr | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. |
| title_full_unstemmed | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. |
| title_short | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. |
| title_sort | Comparison of mouse and human genomes followed by experimental verification yields an estimated 1,019 additional genes. |
| topic | Mamífers Gens Rates (Animals de laboratori) Mammals Genes Rats as laboratory animals |
| topic_facet | Mamífers Gens Rates (Animals de laboratori) Mammals Genes Rats as laboratory animals |
| url | https://hdl.handle.net/2445/192641 |