Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism.
Ghrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regula...
| Authors: | ; ; ; ; ; ; ; |
|---|---|
| Format: | Article |
| Publication date: | 2013 |
| Country: | España |
| Institution: | Instituto de Salud Carlos III (ISCIII) |
| Repository: | Repisalud |
| Language: | English |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/17048 |
| Online Access: | http://hdl.handle.net/20.500.12105/17048 |
| Access Level: | Open access |
| Keyword: | Animales Ghrelina Masculino Piperidinas Ratas Ratas Wistar Ingestión de Alimentos Receptor cannabinoide CB1 Eating Ghrelin Male Piperidines Pyrazoles Rats Rats, Wistar Receptor, Cannabinoid, CB1 Triazoles Animals |
| _version_ | 1878736934030802944 |
|---|---|
| author | Alen, Francisco |
| author2 | Crespo, Inmaculada Ramírez-López, María Teresa Jagerovic, Nadine Goya, Pilar Rodríguez de Fonseca, Fernando Gómez de Heras, Raquel Orio, Laura |
| author2_role | author author author author author author author |
| author_browse | Alen, Francisco Crespo, Inmaculada Goya, Pilar Gómez de Heras, Raquel Jagerovic, Nadine Orio, Laura Ramírez-López, María Teresa Rodríguez de Fonseca, Fernando |
| author_facet | Alen, Francisco Crespo, Inmaculada Ramírez-López, María Teresa Jagerovic, Nadine Goya, Pilar Rodríguez de Fonseca, Fernando Gómez de Heras, Raquel Orio, Laura |
| author_role | author |
| collection | Repisalud |
| description | Ghrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regulate CB1-mediated control of food intake and a functional relationship between hypothalamic ghrelin and cannabinoid CB1 receptor has been proposed. First of all, we investigated brain ghrelin actions on food intake in rats with different metabolic status (negative or equilibrate energy balance). Secondly, we tested a sub-anxiogenic ultra-low dose of the CB1 antagonist SR141716A (Rimonabant) and the peripheral-acting CB1 antagonist LH-21 on ghrelin orexigenic actions. We found that: 1) central administration of ghrelin promotes food intake in free feeding animals but not in 24 h food-deprived or chronically food-restricted animals; 2) an ultra-low dose of SR141716A (a subthreshold dose 75 folds lower than the EC50 for induction of anxiety) completely counteracts the orexigenic actions of central ghrelin in free feeding animals; 3) the peripheral-restricted CB1 antagonist LH-21 blocks ghrelin-induced hyperphagia in free feeding animals. Our study highlights the importance of the animaĺs metabolic status for the effectiveness of ghrelin in promoting feeding, and suggests that the peripheral endocannabinoid system may interact with ghrelińs signal in the control of food intake under equilibrate energy balance conditions. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | ES_97c23d73c28fa3edc658cdeca556e95a |
| institution | Instituto de Salud Carlos III (ISCIII) |
| instname_str | Instituto de Salud Carlos III (ISCIII) |
| language | eng |
| network_acronym_str | ES |
| network_name_str | España |
| oai_identifier_str | oai:repisalud.isciii.es:20.500.12105/17048 |
| publishDate | 2013 |
| publishDateSort | 2013 |
| publisher | Public Library of Science (PLOS) |
| publisherStr | Public Library of Science (PLOS) |
| reponame_str | Repisalud |
| spelling | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. Alen, Francisco Crespo, Inmaculada Ramírez-López, María Teresa Jagerovic, Nadine Goya, Pilar Rodríguez de Fonseca, Fernando Gómez de Heras, Raquel Orio, Laura Animales Ghrelina Masculino Piperidinas Ratas Ratas Wistar Ingestión de Alimentos Receptor cannabinoide CB1 Eating Ghrelin Male Piperidines Pyrazoles Rats Rats, Wistar Receptor, Cannabinoid, CB1 Triazoles Animals Ghrelin is an endogenous regulator of energy homeostasis synthesized by the stomach to stimulate appetite and positive energy balance. Similarly, the endocannabinoid system is part of our internal machinery controlling food intake and energy expenditure. Both peripheral and central mechanisms regulate CB1-mediated control of food intake and a functional relationship between hypothalamic ghrelin and cannabinoid CB1 receptor has been proposed. First of all, we investigated brain ghrelin actions on food intake in rats with different metabolic status (negative or equilibrate energy balance). Secondly, we tested a sub-anxiogenic ultra-low dose of the CB1 antagonist SR141716A (Rimonabant) and the peripheral-acting CB1 antagonist LH-21 on ghrelin orexigenic actions. We found that: 1) central administration of ghrelin promotes food intake in free feeding animals but not in 24 h food-deprived or chronically food-restricted animals; 2) an ultra-low dose of SR141716A (a subthreshold dose 75 folds lower than the EC50 for induction of anxiety) completely counteracts the orexigenic actions of central ghrelin in free feeding animals; 3) the peripheral-restricted CB1 antagonist LH-21 blocks ghrelin-induced hyperphagia in free feeding animals. Our study highlights the importance of the animaĺs metabolic status for the effectiveness of ghrelin in promoting feeding, and suggests that the peripheral endocannabinoid system may interact with ghrelińs signal in the control of food intake under equilibrate energy balance conditions. Public Library of Science (PLOS) http://hdl.handle.net/20.500.12105/17048 |
| spellingShingle | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. Alen, Francisco Animales Ghrelina Masculino Piperidinas Ratas Ratas Wistar Ingestión de Alimentos Receptor cannabinoide CB1 Eating Ghrelin Male Piperidines Pyrazoles Rats Rats, Wistar Receptor, Cannabinoid, CB1 Triazoles Animals |
| title | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. |
| title_full | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. |
| title_fullStr | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. |
| title_full_unstemmed | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. |
| title_short | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. |
| title_sort | Ghrelin-induced orexigenic effect in rats depends on the metabolic status and is counteracted by peripheral CB1 receptor antagonism. |
| topic | Animales Ghrelina Masculino Piperidinas Ratas Ratas Wistar Ingestión de Alimentos Receptor cannabinoide CB1 Eating Ghrelin Male Piperidines Pyrazoles Rats Rats, Wistar Receptor, Cannabinoid, CB1 Triazoles Animals |
| topic_facet | Animales Ghrelina Masculino Piperidinas Ratas Ratas Wistar Ingestión de Alimentos Receptor cannabinoide CB1 Eating Ghrelin Male Piperidines Pyrazoles Rats Rats, Wistar Receptor, Cannabinoid, CB1 Triazoles Animals |
| url | http://hdl.handle.net/20.500.12105/17048 |